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Invitrogen
CD213a1 (IL-13Ra1) Monoclonal Antibody (13MOKA), PE, eBioscience™
-Flow-20170216130641.jpg?time=20240507 "CD213a1 (IL-13Ra1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 2
CD213a1 (IL-13Ra1) Antibody (12-2130-80) in Flow
Splenocytes were cultured and stimulated with Anti-Mouse CD3 and Anti-Mouse CD28 for 3 days or harvested fresh on day 3 and then surface-stained with 0.25 µg of Anti-Mouse CD213a1 (IL-13Ra1) PE. The plot is an overlay of the stimulated cells (purple) and freshly isolated splenocytes (blue). Isotype control staining of the stimulated cells is indicated by the dotted line. Cells in the CD4+ lymphocyte gate we... View More
-Flow-20170216130641.jpg?time=20240507)
-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD213a1 (IL-13Ra1) Antibody (12-2130-80) in Flow")
Product Details
12-2130-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
13MOKA
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10667742
Product Specific Information
Description: The 13MOKA monoclonal antibody reacts with mouse CD213 alpha 1 (interleukin-13 receptor alpha 1, IL-13Ra1). CD213a1 is the low-affinity IL-13 receptor which mediates normal IL-13 inflammatory signaling. It has been shown that CD213a1 binds to IL-13 which then recruits CD124 (IL-4Ralpha) to stabilize the low-affinity interaction and form the active signaling complex. The CD213a1/CD124 complex can also serve as a receptor for IL-4, although the role of CD213a1 in this complex is poorly understood. CD213a1 is expressed on activated B and T cells and other cells.
Applications Reported: This 13MOKA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13MOKA antibody has been tested on mouse splenocytes stimulated for 3 days with Anti-CD3 and Anti-CD28 Functional Grade antibodies. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD antigen CD213a1; CD213a1; IL-13 receptor subunit alpha-1; IL-13RA1; Interleukin-13 receptor subunit alpha-1; Interleukin-13-binding protein; Novel cytokine receptor 4; NR4
Gene Aliases: AI882074; CD213a1; IL-13R-alpha-1; IL-13r[a]; Il13r; Il13ra; Il13ra1; NR4
UniProt ID: (Mouse) O09030
Entrez Gene ID: (Mouse) 16164
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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