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Invitrogen
CD215 (IL-15Ra) Monoclonal Antibody (eBioJM7A4), FITC, eBioscience™
FIGURE: 1 / 1
CD215 (IL-15Ra) Antibody (11-7159-42) in Flow
Normal human peripheral blood cells were stimulated overnight with LPS and Human IFN gamma Recombinant Protein (Product # 14-8319-80). The cells were stained with Anti-Human CD11b PE (Product # 12-0113-42) and Mouse IgG2b K Isotype Control FITC (Product # 11-4732-42) (left) or Anti-Human CD215 (IL-15 Receptor alpha) FITC (right). Cells in the monocyte gate were used for analysis.
Product Details
11-7159-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2b
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioJM7A4
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572516
Product Specific Information
Description: The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL-2Rbeta and gamma chains, which are essential for signal transduction. The IL-15Ralpha receptor is structurally related to IL-2Ralpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2Ralpha and IL-2. In the human, 3 differentially spliced human IL-15Ralpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL-15Ralpha, like that of IL-2Ralpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL-2Rbeta and IL-2Rgamma in the absence of the alpha subunit.
Applications Reported: This eBioJM7A4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioJM7A4 antibody has been pre-titrated and tested by flow cytometric analysis of LPS and IFN gamma-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD215; IL 15 receptor; IL 15 receptor subunit α; IL 15R; IL 15R alpha; IL 15R α; IL-15 receptor subunit alpha; IL15 receptor; IL15 receptor subunit alpha; IL15 receptor subunit α; IL15R; IL15R alpha; IL15R α; Interleukin 15 receptor; interleukin 15 receptor alpha isoform EM2; interleukin 15 receptor alpha isoform IC2; interleukin 15 receptor alpha isoform IC3; interleukin 15 receptor alpha isoform IC4; interleukin 15 receptor alpha isoform IC5; interleukin 15 receptor alpha isoform IC6; interleukin 15 receptor alpha isoform IC7; interleukin 15 receptor alpha isoform IC8; Interleukin 15 receptor subunit α; interleukin 15 receptor, alpha; Interleukin-15 receptor subunit alpha; sIL 15R; sIL-15R-alpha; sIL-15RA; sIL15R; soluble IL 15R; soluble IL15R
Gene Aliases: CD215; IL15RA
UniProt ID: (Human) Q6B0J2
Entrez Gene ID: (Human) 3601
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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