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CD23 Monoclonal Antibody (B3B4), APC

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Published Figures (4)

FIGURE: 1 / 4

CD23 Antibody (MCD2305) in Flow

The Journal of experimental medicine 2015 - Figure 2. B cell-specific deletion of ERK5 reduces B2 cell numbers. (A) Purified splenic FM B cells from mb1-cre + erk5 fl/fl mice and mb1-cre + erk5 wt/wt control mice were analyzed for ERK5 expression by immunoblotting. (B-F) Flow cytometric analysis of B cell populations in mb1-cre + erk5 wt/wt and mb1-cre + erk5 fl/fl mice from the indicated organs, as shown in Fig. S2 . (B) Absolute numbe... View More

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Product Details

MCD2305

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

B3B4

Immunogen

Mouse CD23.

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with sucrose, 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10375870

Target Information

CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Chitinase-3-like 1 regulates T_H2 cells, T_FH cells and IgE responses to helminth infection.

Frontiers in immunology

Curtiss ML,Rosenberg AF,Scharer CD,Mousseau B,Benavides NAB,Bradley JE,León B,Steele C,Randall TD,Lund FE

MCD2305 was used in Flow cytometry/Cell sorting to suggest that Chi3l1 modulates IgE responses, which are known to be highly dependent on IL-4-producing TFH cells.

Sat Aug 19 00:00:00 EDT 2023

The Transcription Factor NFATc1 Supports the Rejection of Heterotopic Heart Allografts.

Frontiers in immunology

Baur J,Otto C,Steger U,Klein-Hessling S,Muhammad K,Pusch T,Murti K,Wismer R,Germer CT,Klein I,Müller N,Serfling E,Avots A

MCD2305 was used in Flow cytometry/Cell sorting to show that the activity of nuclear factors of activated T cells-c1 supports the acute rejection of heterotopic heart allografts.

Wed Nov 20 00:00:00 EST 2019

TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK.

The Journal of experimental medicine

Schweighoffer E,Nys J,Vanes L,Smithers N,Tybulewicz VLJ

MCD2305 was used in flow cytometry to show that TLR4 signals through the BCR leading to activation of SYK, ERK, and AKT as well as through MYD88 leading to activation of NFkappaB

Mon May 01 00:00:00 EDT 2017

BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival.

The Journal of experimental medicine

Jacque E,Schweighoffer E,Tybulewicz VL,Ley SC

MCD2305 was used in flow cytometry to study the effect of ERK1/2 and ERK5 activation on BAFF-induced B cell survival

Mon Jun 01 00:00:00 EDT 2015

IKK-induced NF-κB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen.

The Journal of experimental medicine

Jacque E,Schweighoffer E,Visekruna A,Papoutsopoulou S,Janzen J,Zillwood R,Tarlinton DM,Tybulewicz VL,Ley SC

MCD2305 was used in flow cytometry to study IκB kinase-induced proteolysis of NF-κB1 p105 in B cells using Nfkb1(SSAA/SSAA) mice.

Mon Sep 22 00:00:00 EDT 2014

IL-4 regulates Bim expression and promotes B cell maturation in synergy with BAFF conferring resistance to cell death at negative selection checkpoints.

Journal of immunology (Baltimore, Md. : 1950)

Granato A,Hayashi EA,Baptista BJ,Bellio M,Nobrega A

MCD2305 was used in Flow cytometry/Cell sorting to analyze the effect of IL-4 on B cell maturation, and its influence on BCR-mediated negative selection.

Sun Jun 15 00:00:00 EDT 2014

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

MCD2305 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

TLR4 promotes B cell maturation: independence and cooperation with B lymphocyte-activating factor.

Journal of immunology (Baltimore, Md. : 1950)

Hayashi EA,Granato A,Paiva LS,Bertho AL,Bellio M,Nobrega A

MCD2305 was used in flow cytometry to report the cell surface markers and functional properties of B cells matured in vitro with LPS

Sat May 01 00:00:00 EDT 2010

IkappaBalpha is required for marginal zone B cell lineage development.

European journal of immunology

Ellinghaus U,Rupec RA,Pabst O,Ignatius R,Förster R,Dörken B,Jundt F

Published figure using CD23 monoclonal antibody (Product # MCD2305) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD23; FC epsilon RII; Fc-epsilon-RII; FceRII; Low affinity immunoglobulin epsilon Fc receptor; low-affinity IgE receptor; Lymphocyte IgE receptor

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Gene Aliases: CD23; Fce2; Fcer2; Fcer2a; Ly-42

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UniProt ID: (Mouse) P20693

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Entrez Gene ID: (Mouse) 14128

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Function(s)

IgE binding carbohydrate binding metal ion binding membrane traffic protein

Process(es)

positive regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of nitric-oxide synthase activity positive regulation of killing of cells of other organism positive regulation of nitric-oxide synthase biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD23 Monoclonal Antibody (B3B4), APC

CD23 Antibody (MCD2305) in Flow

Product Details

MCD2305

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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