Invitrogen

CD23 Monoclonal Antibody (SP23)

CD23 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

CD23 Antibody (MA5-14572) in IHC (P)

Formalin-fixed, paraffin-embedded human tonsil stained with CD23 using AEC chromogen. Note membrane staining of B-cells and dendritic cell

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Product Details

MA5-14572

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:100

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

1:2

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP23

Immunogen

Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.076 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986348

Product Specific Information

The MA5-14572 immunogen is recombinant protein encoding human CD23 48-248aa.

Target Information

CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tertiary lymphoid structures predict the prognosis and immunotherapy response of cholangiocarcinoma.

Frontiers in immunology

Shang T,Jiang T,Lu T,Wang H,Cui X,Pan Y,Xu M,Pei M,Ding Z,Feng X,Lin Y,Li X,Tan Y,Feng F,Dong H,Wang H,Dong L

MA5-14572 was used in Immunohistochemistry to investigate the prognostic value and clinical relevance of TLS in CCA using a surgery cohort containing 471 CCA patients (cohort 1) and an immunotherapy cohort containing 100 CCA patients (cohort 2).

Mon May 29 00:00:00 EDT 2023

TGF-β-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures.

Immunity

Chaurio RA,Anadon CM,Lee Costich T,Payne KK,Biswas S,Harro CM,Moran C,Ortiz AC,Cortina C,Rigolizzo KE,Sprenger KB,Mine JA,Innamarato P,Mandal G,Powers JJ,Martin A,Wang Z,Mehta S,Perez BA,Li R,Robinson J,Kroeger JL,Curiel TJ,Yu X,Rodriguez PC,Conejo-Garcia JR

MA5-14572 was used in Immunohistochemistry-immunofluorescence to examine the impact of regulation of PD-1 expression by the genomic organizer SATB1 on Tfh cell differentiation.

Tue Jan 11 00:00:00 EST 2022

Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1.

Annals of diagnostic pathology

Agaimy A,Michal M,Hadravsky L,Michal M

MA514572 was used in immunohistochemistry - paraffin section to characterize cases of follicular dendritic cell sarcoma

Mon Aug 01 00:00:00 EDT 2016

A reference image-based method for optimization of clinical immunohistochemistry.

Histopathology

Kap M,Lam KH,Ewing-Graham P,Riegman P

MA5-14572 was used in immunohistochemistry to describe a new method for immunohistochemistry optimization

Sat Aug 01 00:00:00 EDT 2015

Inadequate T follicular cell help impairs B cell immunity during HIV infection.

Nature medicine

Cubas RA,Mudd JC,Savoye AL,Perreau M,van Grevenynghe J,Metcalf T,Connick E,Meditz A,Freeman GJ,Abesada-Terk G,Jacobson JM,Brooks AD,Crotty S,Estes JD,Pantaleo G,Lederman MM,Haddad EK

MA5-14572 was used in immunohistochemistry to study the expansion of follicular helper T-cells that fail to provide adequate help to B-cells in individuals with HIV infection

Mon Apr 01 00:00:00 EDT 2013

The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses.

Journal of autoimmunity

Piconese S,Costanza M,Tripodo C,Sangaletti S,Musio S,Pittoni P,Poliani PL,Burocchi A,Passafaro AL,Gorzanelli A,Vitali C,Chiodoni C,Barnaba V,Pedotti R,Colombo MP

MA5-14572 was used in immunohistochemistry to investigate the role of SPARC in lymphoid tissue during immune response

Thu Dec 01 00:00:00 EST 2011

Simultaneous activity assay of two transglutaminase isozymes, blood coagulation factor XIII and transglutaminase 2, by use of fibrinogen arrays.

Analytical chemistry

Kwon MH,Jung SH,Kim YM,Ha KS

MA5-14572 was used in immunohistochemistry to investigate the expression pattern of SPARC in reactive lymph nodes during immune response

Tue Nov 15 00:00:00 EST 2011

Composite biclonal marginal zone lymphoma of lung and chronic lymphocytic leukemia: pathologic, phenotypic, cytogenetic, and molecular study.

International journal of surgical pathology

Bhagavathi S,Micale MA,Douglas-Nikitin V,Ballouz S,Neumann K,Blenc AM

MA5-14572 was used in immunohistochemistry to investigate the coexistence of mucosa-associated lymphoid tissue lymphoma and chronic lymphocytic leukemia in a patient

Wed Jun 01 00:00:00 EDT 2011

Histological assessment of PAXgene tissue fixation and stabilization reagents.

PloS one

Kap M,Smedts F,Oosterhuis W,Winther R,Christensen N,Reischauer B,Viertler C,Groelz D,Becker KF,Zatloukal K,Langer R,Slotta-Huspenina J,Bodo K,de Jong B,Oelmuller U,Riegman P

MA5-14572 was used in immunohistochemistry - paraffin section to examine the suitability of PAXgene tissue fixation for (immuno)histological methods

Mon Mar 26 00:00:00 EDT 2012

Cyclin D1 expression in B-cell lymphomas.

Experimental hematology

Gladkikh A,Potashnikova D,Korneva E,Khudoleeva O,Vorobjev I

MA5-14572 was used in immunohistochemistry to study the expression of cyclin D1 in B-cell lymphomas

Mon Nov 01 00:00:00 EDT 2010

Pediatric lymphomas in Brazil.

Clinics (Sao Paulo, Brazil)

Gualco G,Klumb CE,Barber GN,Weiss LM,Bacchi CE

MA5-14572 was used in immunohistochemistry to study the characteristics of pediatric lymphomas in the Brazilian population

Wed Aug 10 00:00:00 EDT 2011

Burkitt lymphoma in Brazil is characterized by geographically distinct clinicopathologic features.

American journal of clinical pathology

Queiroga EM,Gualco G,Weiss LM,Dittmer DP,Araujo I,Klumb CE,Harrington WJ,Bacchi CE

MA5-14572 was used in immunohistochemistry to examine the unique geographic and immunohistochemical features of Burkitt lymphoma in Brazil

Mon Dec 01 00:00:00 EST 2008

Sarcoma of follicular dendritic cells with features of sinus lining cells--a new subtype of reticulum cell sarcoma?

Virchows Archiv : an international journal of pathology

Krokowski M,Merz H,Thorns C,Bernd HW,Schade U,Le Tourneau A,Diebold J,Feller AC

MA5-14572 was used in immunohistochemistry to characterize a potentially new subtype of reticulum cell sarcoma

Thu May 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: BLAST-2; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD23; CD23 antigen; Fc epsilon receptor II; FC epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc-epsilon-RII; FceRII; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; Low affinity immunoglobulin epsilon Fc receptor; low-affinity IgE receptor; Lymphocyte IgE receptor

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Gene Aliases: BLAST-2; CD23; CD23A; CLEC4J; FCE2; FCER2; Fcer2a; IGEBF; Ly-42

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UniProt ID: (Human) P06734, (Mouse) P20693

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Entrez Gene ID: (Human) 2208, (Mouse) 14128

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Function(s)

integrin binding protein binding IgE binding carbohydrate binding metal ion binding membrane traffic protein

Process(es)

positive regulation of humoral immune response mediated by circulating immunoglobulin Notch signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of killing of cells of other organism positive regulation of nitric-oxide synthase biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD23 Monoclonal Antibody (SP23)

CD23 Antibody (MA5-14572) in IHC (P)

Product Details

MA5-14572

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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