Invitrogen

CD24 Monoclonal Antibody (M1/69), PE-Cyanine7, eBioscience™

FIGURE: 1 / 55

CD24 Antibody (25-0242-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3 eFluor® 450 (Product # 48-0032-82) and 0.06 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) (left) or 0.06 µg of Anti-Mouse CD24 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

25-0242-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 40 publications 40 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M1/69

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853806

Product Specific Information

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This M1/69 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.

Nature communications

Chiang HY,Lu HH,Sudhakar JN,Chen YW,Shih NS,Weng YT,Shui JW

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue Feb 15 00:00:00 EST 2022

Population-wide gene disruption in the murine lung epithelium via AAV-mediated delivery of CRISPR-Cas9 components.

Molecular therapy. Methods & clinical development

Chen H,Durinck S,Patel H,Foreman O,Mesh K,Eastham J,Caothien R,Newman RJ,Roose-Girma M,Darmanis S,Warming S,Lattanzi A,Liang Y,Haley B

25-0242-82 was used in flow cytometry to demonstrate a uniquely effective approach for somatic engineering of the murine lung, which will greatly facilitate the modeling of disease and therapeutic intervention.

Thu Dec 08 00:00:00 EST 2022

FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver.

STAR protocols

Braun TW,Kuoch MK,Khandros E,Li H

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Fri Mar 18 00:00:00 EDT 2022

Dormant Nfatc1 reporter-marked basal stem/progenitor cells contribute to mammary lobuloalveoli formation.

iScience

Liu R,Hu H,McNeil M,Xu J,Bi X,Lou P,Guerrero-Juarez CF,Dai X,Plikus MV,Shuai J,Yu Z,Lv C

25-0242-82 was used in Flow cytometry/Cell sorting to show that Nfatc1 expression marks a rare population of mammary epithelial cells with the majority being basal epithelial cells.

Fri Mar 18 00:00:00 EDT 2022

IRE1α drives lung epithelial progenitor dysfunction to establish a niche for pulmonary fibrosis.

American journal of physiology. Lung cellular and molecular physiology

Auyeung VC,Downey MS,Thamsen M,Wenger TA,Backes BJ,Sheppard D,Papa FR

25-0242-82 was used in Flow cytometry/Cell sorting to show that the bifunctional kinase/RNase-IRE1α-a central mediator of the unfolded protein response (UPR) to endoplasmic reticulum (ER) stress is a critical promoter of DATP abundance and function.

Fri Apr 01 00:00:00 EDT 2022

Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells.

Nature communications

Gerdes P,Lim SM,Ewing AD,Larcombe MR,Chan D,Sanchez-Luque FJ,Walker L,Carleton AL,James C,Knaupp AS,Carreira PE,Nefzger CM,Lister R,Richardson SR,Polo JM,Faulkner GJ

25-0242-82 was used in flow cytometry to reveal the complete spectrum and potential significance of mutations acquired by miPSCs.

Sat Dec 03 00:00:00 EST 2022

Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.

Frontiers in cell and developmental biology

Shao C,Lou P,Liu R,Bi X,Li G,Yang X,Sheng X,Xu J,Lv C,Yu Z

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.

Frontiers in cell and developmental biology

Shao C,Lou P,Liu R,Bi X,Li G,Yang X,Sheng X,Xu J,Lv C,Yu Z

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Testosterone metabolites differentially regulate obesogenesis and fat distribution.

Molecular metabolism

Sebo ZL,Rodeheffer MS

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium.

Development (Cambridge, England)

Gagniac L,Rusidzé M,Boudou F,Cagnet S,Adlanmerini M,Jeannot P,Gaide N,Giton F,Besson A,Weyl A,Gourdy P,Raymond-Letron I,Arnal JF,Brisken C,Lenfant F

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Transcriptomic and proteomic signatures of stemness and differentiation in the colon crypt.

Communications biology

Habowski AN,Flesher JL,Bates JM,Tsai CF,Martin K,Zhao R,Ganesan AK,Edwards RA,Shi T,Wiley HS,Shi Y,Hertel KJ,Waterman ML

25-0242 was used in Flow cytometry/Cell sorting to show that alternative mRNA splicing and polyadenylation dominate changes in the transcriptome as stem cells differentiate into progenitors.

Wed Aug 19 00:00:00 EDT 2020

Transcriptomic and proteomic signatures of stemness and differentiation in the colon crypt.

Communications biology

Habowski AN,Flesher JL,Bates JM,Tsai CF,Martin K,Zhao R,Ganesan AK,Edwards RA,Shi T,Wiley HS,Shi Y,Hertel KJ,Waterman ML

25-0242 was used in Flow cytometry/Cell sorting to show that alternative mRNA splicing and polyadenylation dominate changes in the transcriptome as stem cells differentiate into progenitors.

Wed Aug 19 00:00:00 EDT 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Survival of Single Positive Thymocytes Depends upon Developmental Control of RIPK1 Kinase Signaling by the IKK Complex Independent of NF-κB.

Immunity

Webb LV,Barbarulo A,Huysentruyt J,Vanden Berghe T,Takahashi N,Ley S,Vandenabeele P,Seddon B

25-0242-82 was used in Flow Cytometry to show that IKK was required to protect Rel-deficient thymocytes from RIPK1-dependent cell death, underscoring the NF-κB-independent function of IKK during thymic development.

Tue Feb 19 00:00:00 EST 2019

An Nfil3-Zeb2-Id2 pathway imposes Irf8 enhancer switching during cDC1 development.

Nature immunology

Bagadia P,Huang X,Liu TT,Durai V,Grajales-Reyes GE,Nitschké M,Modrusan Z,Granja JM,Satpathy AT,Briseño CG,Gargaro M,Iwata A,Kim S,Chang HY,Shaw AS,Murphy TL,Murphy KM

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Lifelong n-3 Polyunsaturated Fatty Acid Exposure Modulates Size of Mammary Epithelial Cell Populations and Expression of Caveolae Resident Proteins in Fat-1 Mice.

Nutrients

Hillyer LM,Kang JX,Ma DWL

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2019

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

25-0242-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation.

Nature communications

Zwick RK,Rudolph MC,Shook BA,Holtrup B,Roth E,Lei V,Van Keymeulen A,Seewaldt V,Kwei S,Wysolmerski J,Rodeheffer MS,Horsley V

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue Sep 04 00:00:00 EDT 2018

Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells.

Genes

Hrckulak D,Janeckova L,Lanikova L,Kriz V,Horazna M,Babosova O,Vojtechova M,Galuskova K,Sloncova E,Korinek V

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

A Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic Leukemia.

Cancer cell

Robles-Valero J,Lorenzo-Martín LF,Menacho-Márquez M,Fernández-Pisonero I,Abad A,Camós M,Toribio ML,Espinosa L,Bigas A,Bustelo XR

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Mon Nov 13 00:00:00 EST 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

25-0242 was used in Flow cytometry/Cell sorting to demonstrate that the effects of cytokines regulating haematopoietic stem cell functions are dependent on the producing cell sources.

Wed Mar 01 00:00:00 EST 2017

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Metabolic shifts in residual breast cancer drive tumor recurrence.

The Journal of clinical investigation

Havas KM,Milchevskaya V,Radic K,Alladin A,Kafkia E,Garcia M,Stolte J,Klaus B,Rotmensz N,Gibson TJ,Burwinkel B,Schneeweiss A,Pruneri G,Patil KR,Sotillo R,Jechlinger M

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Skin Adipocyte Stem Cell Self-Renewal Is Regulated by a PDGFA/AKT-Signaling Axis.

Cell stem cell

Rivera-Gonzalez GC,Shook BA,Andrae J,Holtrup B,Bollag K,Betsholtz C,Rodeheffer MS,Horsley V

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation.

Proceedings of the National Academy of Sciences of the United States of America

Mukhopadhyay C,Triplett A,Bargar T,Heckman C,Wagner KU,Naramura M

25-0242 was used in Flow cytometry/Cell sorting to investigate the protection of MECs from proteotoxic stress.

Tue Dec 20 00:00:00 EST 2016

Batf3 maintains autoactivation of Irf8 for commitment of a CD8α(+) conventional DC clonogenic progenitor.

Nature immunology

Grajales-Reyes GE,Iwata A,Albring J,Wu X,Tussiwand R,Kc W,Kretzer NM,Briseño CG,Durai V,Bagadia P,Haldar M,Schönheit J,Rosenbauer F,Murphy TL,Murphy KM

25-0242 was used in Flow cytometry/Cell sorting to investigate the role of the transcription factors Batf3 and IRF8 in the development of CD8α(+) conventional dendritic cells..

Wed Jul 01 00:00:00 EDT 2015

Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.

Nature cell biology

Jeffery E,Church CD,Holtrup B,Colman L,Rodeheffer MS

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement.

Immunology

Ayre DC,Elstner M,Smith NC,Moores ES,Hogan AM,Christian SL

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement.

Immunology

Ayre DC,Elstner M,Smith NC,Moores ES,Hogan AM,Christian SL

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.

The Journal of biological chemistry

Shahi P,Slorach EM,Wang CY,Chou J,Lu A,Ruderisch A,Werb Z

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Fri Feb 06 00:00:00 EST 2015

FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.

Development (Cambridge, England)

Zhang X,Martinez D,Koledova Z,Qiao G,Streuli CH,Lu P

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain.

eLife

Mich JK,Signer RA,Nakada D,Pineda A,Burgess RJ,Vue TY,Johnson JE,Morrison SJ

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Wed May 07 00:00:00 EDT 2014

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

Characterization of the adipocyte cellular lineage in vivo.

Nature cell biology

Berry R,Rodeheffer MS

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Mon Dec 08 00:00:00 EST 2014

FLT3-ITDs instruct a myeloid differentiation and transformation bias in lymphomyeloid multipotent progenitors.

Cell reports

Mead AJ,Kharazi S,Atkinson D,Macaulay I,Pecquet C,Loughran S,Lutteropp M,Woll P,Chowdhury O,Luc S,Buza-Vidas N,Ferry H,Clark SA,Goardon N,Vyas P,Constantinescu SN,Sitnicka E,Nerlov C,Jacobsen SE

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Thu Jun 27 00:00:00 EDT 2013

Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.

Cancer cell

Xu K,Usary J,Kousis PC,Prat A,Wang DY,Adams JR,Wang W,Loch AJ,Deng T,Zhao W,Cardiff RD,Yoon K,Gaiano N,Ling V,Beyene J,Zacksenhaus E,Gridley T,Leong WL,Guidos CJ,Perou CM,Egan SE

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue May 15 00:00:00 EDT 2012

Restricted expression of new guanine nucleotide exchange factor Zizimin2 in aged acquired immune system.

Nagoya journal of medical science

Jia Y,Sakabe I,Matsuda T,Hayakawa T,Maruyama M

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland.

Developmental dynamics : an official publication of the American Association of Anatomists

Anderson LH,Boulanger CA,Smith GH,Carmeliet P,Watson CJ

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2011

A multifunctional 3D co-culture system for studies of mammary tissue morphogenesis and stem cell biology.

PloS one

Campbell JJ,Davidenko N,Caffarel MM,Cameron RE,Watson CJ

Published figure using CD24 monoclonal antibody (Product # 25-0242-82) in Flow Cytometry

Mon Mar 05 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen

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Gene Aliases: Cd24; Cd24a; HSA; Ly-52; nectadrin

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UniProt ID: (Mouse) P24807

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Entrez Gene ID: (Mouse) 12484

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Function(s)

protein binding protein kinase binding carbohydrate binding protein tyrosine kinase activator activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD24 Monoclonal Antibody (M1/69), PE-Cyanine7, eBioscience™

CD24 Antibody (25-0242-82) in Flow

Product Details

25-0242-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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