Invitrogen

CD24 Monoclonal Antibody (M1/69), Brilliant Ultra Violet™ 737, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

367-0242-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet™ 737, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M1/69

Conjugate

Brilliant Ultra Violet™ 737 Brilliant Ultra Violet™ 737 Brilliant Ultra Violet™ 737

Excitation/Emission Max

350/740 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2895951

Product Specific Information

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This M1/69 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.

Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen

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Gene Aliases: Cd24; Cd24a; HSA; Ly-52; nectadrin

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UniProt ID: (Mouse) P24807

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Entrez Gene ID: (Mouse) 12484

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Function(s)
protein binding protein kinase binding carbohydrate binding protein tyrosine kinase activator activity
Process(es)
response to hypoxia cell activation regulation of cytokine-mediated signaling pathway response to molecule of bacterial origin pre-B cell differentiation leukocyte migration involved in inflammatory response positive regulation of T cell mediated immune response to tumor cell positive regulation of inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuromuscular synaptic transmission negative regulation of neuroblast proliferation apoptotic mitochondrial changes positive regulation of neuron projection development cell migration regulation of cell-cell adhesion positive regulation of cell-cell adhesion apoptotic nuclear changes negative regulation of B cell proliferation neuron projection development T cell costimulation chemokine receptor transport out of membrane raft negative regulation of transforming growth factor beta3 production positive regulation of integrin activation positive regulation of cell adhesion mediated by integrin regulation of cell-cell adhesion mediated by integrin positive regulation of cell-cell adhesion mediated by integrin negative regulation of erythrocyte clearance homotypic cell-cell adhesion negative regulation of erythrocyte aggregation positive regulation of T cell homeostatic proliferation positive regulation of activated T cell proliferation regulation of phosphorylation cholesterol homeostasis positive regulation of MAP kinase activity regulation of MAPK cascade response to estrogen regulation of B cell differentiation negative regulation of neuron differentiation respiratory burst negative regulation of T cell homeostatic proliferation positive regulation of alpha-beta T cell proliferation synaptic vesicle endocytosis positive regulation of peptidyl-tyrosine phosphorylation negative regulation of neurogenesis positive regulation of calcium-mediated signaling positive regulation of protein tyrosine kinase activity apoptotic signaling pathway
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