Invitrogen

CD24 Monoclonal Antibody (M1/69), Alexa Fluor™ 532, eBioscience™

FIGURE: 1 / 15

CD24 Antibody (58-0242-82) in Flow

C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0032-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 532 (Product # 58-4031-80) (left) or 0.125 µg of CD24 Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

58-0242-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 532, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M1/69

Conjugate

Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532

Excitation/Emission Max

534/553 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815268

Product Specific Information

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This M1/69 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.