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CD244 Antibody (PA5-47054) in IHC (P)
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2020 -
Figure 4. Fluorescence immunohistochemical staining demonstrates expression of CD44 on CD150-positive HSCs (A, yellow arrows). Chemoattractant OPN and its receptor CD44 are expressed in a peri-arteriolar HSC niche in human bone marrow (B) and in a peri-arteriolar GSC niche in glioblastoma (C). Chemoattractant OPN is expressed intracellularly in HSCs and extracellularly and its receptor CD44 is abundantly ex... View More
Product Details
PA5-47054
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse 2B4/CD244/SLAMF4 His17-Asn221
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608062
Product Specific Information
In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) 2B4 is observed and less than 1% cross-reactivity with rhSLAM is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity. CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells. Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease. Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome. Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD244; CD244 long isoform; CD244 short isoform; Natural killer cell receptor 2B4; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR Nmrk; NKR2B4; non MHC restricted killing associated; Non-MHC restricted killing associated; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4
Gene Aliases: 2B4; C9.1; Cd244; F730046O15Rik; Ly90; NAIL; NKR2B4; Nmrk; SLAMF4
UniProt ID: (Mouse) Q07763
Entrez Gene ID: (Mouse) 18106
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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