Invitrogen

CD25 Monoclonal Antibody (CD25-4E3), APC-eFluor™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

8 References

View all (229) CD25 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 11

CD25 Antibody (47-0257-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human/Non-Human Primate CD25 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD25 Antibody — Staining of stimulated human peripheral blood cells. As expected based on known expression patterns, CD25 clone CD25-4E3 staining is significantly increased in CD4+ T cells after stimulation. Details: Normal human peripheral blood cells were unstimulated (blue histogram) or stimulated for 3 days with Concanavalin A (Con A) Solution (500X) and Lipopolysaccharide (LPS) Solution (500X) (purple histogram). Cells were stained with Fixable Viability Dye eFluor 780, CD4 (clone SK3) and CD25 (clone CD25-4E3). Viable cells in the CD4+ gate were used for analysis. {TM}

Product Details

47-0257-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD25-4E3

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573952

Product Specific Information

Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes.

This monoclonal antibody is cross-reactive to rhesus monkey.

Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

47-0257-42 was used in Flow Cytometry to demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α.

Mon Dec 06 00:00:00 EST 2021

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

47-0257-42 was used in Flow Cytometry to demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α.

Mon Dec 06 00:00:00 EST 2021

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Remote Limb Ischemic Postconditioning Protects Against Ischemic Stroke by Promoting Regulatory T Cells Thriving.

Journal of the American Heart Association

Yu HH,Ma XT,Ma X,Chen M,Chu YH,Wu LJ,Wang W,Qin C,Tian DS

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Tue Nov 16 00:00:00 EST 2021

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

47-0257 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

Tumor specific regulatory T cells in the bone marrow of breast cancer patients selectively upregulate the emigration receptor S1P1.

Cancer immunology, immunotherapy : CII

Rathinasamy A,Domschke C,Ge Y,Böhm HH,Dettling S,Jansen D,Lasitschka F,Umansky L,Gräler MH,Hartmann J,Herold-Mende C,Schuetz F,Beckhove P

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.

Oncoimmunology

Schmidt HH,Ge Y,Hartmann FJ,Conrad H,Klug F,Nittel S,Bernhard H,Domschke C,Schuetz F,Sohn C,Beckhove P

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; sIL 2R; soluble IL 2 receptor; TAC antigen

View more View less

Gene Aliases: CD25; IDDM10; IL2R; IL2RA; IMD41; p55; TCGFR

View more View less

UniProt ID: (Human) P01589

View more View less

Entrez Gene ID: (Human) 3559

View more View less

Function(s)

interleukin-2 receptor activity Ras guanyl-nucleotide exchange factor activity drug binding interleukin-2 binding transmembrane signal receptor

Process(es)

MAPK cascade inflammatory response to antigenic stimulus regulation of T cell tolerance induction apoptotic process activation-induced cell death of T cells inflammatory response immune response cell surface receptor signaling pathway Notch signaling pathway cell proliferation interleukin-2-mediated signaling pathway positive regulation of activated T cell proliferation negative regulation of T cell proliferation positive regulation of GTPase activity positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD25 Monoclonal Antibody (CD25-4E3), APC-eFluor™ 780, eBioscience™

CD25 Antibody (47-0257-42) in Flow

Product Details

47-0257-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!