Invitrogen

CD25 Monoclonal Antibody (CD25-4E3), APC-eFluor™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

8 References

View all (229) CD25 antibodies

Datasheet

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FIGURE: 1 / 11

CD25 Antibody (47-0257-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human/Non-Human Primate CD25 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD25 Antibody — Staining of stimulated human peripheral blood cells. As expected based on known expression patterns, CD25 clone CD25-4E3 staining is significantly increased in CD4+ T cells after stimulation. Details: Normal human peripheral blood cells were unstimulated (blue histogram) or stimulated for 3 days with Concanavalin A (Con A) Solution (500X) and Lipopolysaccharide (LPS) Solution (500X) (purple histogram). Cells were stained with Fixable Viability Dye eFluor 780, CD4 (clone SK3) and CD25 (clone CD25-4E3). Viable cells in the CD4+ gate were used for analysis. {TM}

Product Details

47-0257-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD25-4E3

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573952

Product Specific Information

Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes.

This monoclonal antibody is cross-reactive to rhesus monkey.

Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

47-0257-42 was used in Flow Cytometry to demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α.

Mon Dec 06 00:00:00 EST 2021

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

47-0257-42 was used in Flow Cytometry to demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α.

Mon Dec 06 00:00:00 EST 2021

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Remote Limb Ischemic Postconditioning Protects Against Ischemic Stroke by Promoting Regulatory T Cells Thriving.

Journal of the American Heart Association

Yu HH,Ma XT,Ma X,Chen M,Chu YH,Wu LJ,Wang W,Qin C,Tian DS

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Tue Nov 16 00:00:00 EST 2021

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

47-0257 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

Tumor specific regulatory T cells in the bone marrow of breast cancer patients selectively upregulate the emigration receptor S1P1.

Cancer immunology, immunotherapy : CII

Rathinasamy A,Domschke C,Ge Y,Böhm HH,Dettling S,Jansen D,Lasitschka F,Umansky L,Gräler MH,Hartmann J,Herold-Mende C,Schuetz F,Beckhove P

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.

Oncoimmunology

Schmidt HH,Ge Y,Hartmann FJ,Conrad H,Klug F,Nittel S,Bernhard H,Domschke C,Schuetz F,Sohn C,Beckhove P

Published figure using CD25 monoclonal antibody (Product # 47-0257-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; sIL 2R; soluble IL 2 receptor; TAC antigen

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Gene Aliases: CD25; IDDM10; IL2R; IL2RA; IMD41; p55; TCGFR

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UniProt ID: (Human) P01589

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Entrez Gene ID: (Human) 3559

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Function(s)

interleukin-2 receptor activity Ras guanyl-nucleotide exchange factor activity drug binding interleukin-2 binding transmembrane signal receptor

Process(es)

MAPK cascade inflammatory response to antigenic stimulus regulation of T cell tolerance induction apoptotic process activation-induced cell death of T cells inflammatory response immune response cell surface receptor signaling pathway Notch signaling pathway cell proliferation interleukin-2-mediated signaling pathway positive regulation of activated T cell proliferation negative regulation of T cell proliferation positive regulation of GTPase activity positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD25 Monoclonal Antibody (CD25-4E3), APC-eFluor™ 780, eBioscience™

CD25 Antibody (47-0257-42) in Flow

Product Details

47-0257-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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