Invitrogen

CD26 Monoclonal Antibody (H194-112 (H194-112.12.4.4.5)), PerCP-Cyanine5.5, eBioscience™

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Antibody Testing Data (1)
Published Figures (2)

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CD26 Antibody (45-0261-82) in Flow

Staining of C57BL/6 thymocytes with 0.5 µg of Rat IgG2a kappa Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (blue histogram) or 0.5 µg of Anti-Mouse CD26 PerCP-Cyanine5-5 (purple histogram). Total viable cells were used for analysis.

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Product Details

45-0261-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H194-112 (H194-112.12.4.4.5)

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548738

Product Specific Information

Description: The monoclonal antibody H194-112 recognizes mouse CD26, a thymocyte-activating molecule (THAM). CD26 is a surface expressed and soluble ectoenzyme (dipeptidyl peptidase IV) that cleaves dipeptides from the amino terminus of proteins. In particular CD26 has been shown to cleave CXCL12, CCL22, neuropeptide Y and glucagon. CD26 is involved in adhesion, apoptosis, disease regulation, and engraftment. Expression is regulated during thymic development at the messenger RNA level. Additionally, CD26 protein is found on thymocytes, a percentage of resting B cells, stimulated B cells, NK cells and a percentage of resting and activated T cells and other epithelial cell types. On human cells more CD26 protein is found on Th2 cells. Functional CD26 is expressed on Sca-1+c-Kit+lin- cells. This expression has been shown to correlate with mobilization of progenitor cells. Inhibition of CD26 activity through a variety of compounds enhances allogenic stem cell homing and engraftment.

This antibody is reported to have activating function.

Applications Reported: This H194-112 (H194-112.12.4.4.5) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H194-112 (H194-112.12.4.4.5) antibody has been tested by flow cytometric analysis of mouse splenocytes or thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

45-0261 was used in Flow cytometry/Cell sorting to test the hypothesis that the development of resistance to Fas-induced apoptosis by lung fibroblasts and myofibroblasts contibributes to their accumulation in the distal lung tissues of IPF patients.

Tue Dec 08 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Tue Dec 08 00:00:00 EST 2020

Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis.

Cell reports

Leavitt T,Hu MS,Borrelli MR,Januszyk M,Garcia JT,Ransom RC,Mascharak S,desJardins-Park HE,Litzenburger UM,Walmsley GG,Marshall CD,Moore AL,Duoto B,Adem S,Foster DS,Salhotra A,Shen AH,Griffin M,Shen EZ,Barnes LA,Zielins ER,Maan ZN,Wei Y,Chan CKF,Wan DC,Lorenz HP,Chang HY,Gurtner GC,Longaker MT

45-0261-82 was used in Flow Cytometry to identify and characterise a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.

Tue Nov 10 00:00:00 EST 2020

TGFβ-blockade uncovers stromal plasticity in tumors by revealing the existence of a subset of interferon-licensed fibroblasts.

Nature communications

Grauel AL,Nguyen B,Ruddy D,Laszewski T,Schwartz S,Chang J,Chen J,Piquet M,Pelletier M,Yan Z,Kirkpatrick ND,Wu J,deWeck A,Riester M,Hims M,Geyer FC,Wagner J,MacIsaac K,Deeds J,Diwanji R,Jayaraman P,Yu Y,Simmons Q,Weng S,Raza A,Minie B,Dostalek M,Chikkegowda P,Ruda V,Iartchouk O,Chen N,Thierry R,Zhou J,Pruteanu-Malinici I,Fabre C,Engelman JA,Dranoff G,Cremasco V

45-0261 was used in Flow cytometry/Cell sorting to perform an unbiased interrogation of tumor mesenchymal cells, delineating the co-existence of distinct subsets of cancer-associated fibroblasts (CAFs) in the microenvironment of murine carcinomas, each endowed with unique phenotypic features and functions.

Wed Dec 09 00:00:00 EST 2020

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Sun Apr 01 00:00:00 EDT 2018

Fibroblast state switching orchestrates dermal maturation and wound healing.

Molecular systems biology

Rognoni E,Pisco AO,Hiratsuka T,Sipilä KH,Belmonte JM,Mobasseri SA,Philippeos C,Dilão R,Watt FM

45-0261 was used in Flow cytometry/Cell sorting to examine the role of extracellular matrix deposition and fibroblast proliferation in determining dermal architecture.

Wed Aug 29 00:00:00 EDT 2018

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

45-0261 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone.

Thu Jun 01 00:00:00 EDT 2017

MyD88 signaling in dendritic cells and the intestinal epithelium controls immunity against intestinal infection with C. rodentium.

PLoS pathogens

Friedrich C,Mamareli P,Thiemann S,Kruse F,Wang Z,Holzmann B,Strowig T,Sparwasser T,Lochner M

45-0261 was used in Flow cytometry/Cell sorting to suggest that MyD88 signaling in DC and IEC is both essential and sufficient to induce a full spectrum of host responses upon intestinal infection with C. rodentium.

Mon May 01 00:00:00 EDT 2017

Distinct fibroblast lineages determine dermal architecture in skin development and repair.

Nature

Driskell RR,Lichtenberger BM,Hoste E,Kretzschmar K,Simons BD,Charalambous M,Ferron SR,Herault Y,Pavlovic G,Ferguson-Smith AC,Watt FM

45-0261 was used in Flow cytometry/Cell sorting to explain why wounding is linked to formation of ECM-rich scar tissue that lacks hair follicles.

Thu Dec 12 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CD26; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV; DPP IV; DPP-IV; T-cell activation antigen CD26; THAM; Thymocyte-activating molecule

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Gene Aliases: Cd26; Dpp-4; Dpp4; THAM

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UniProt ID: (Mouse) P28843

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Entrez Gene ID: (Mouse) 13482

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Function(s)

virus receptor activity protease binding aminopeptidase activity serine-type endopeptidase activity receptor binding collagen binding peptidase activity serine-type peptidase activity dipeptidyl-peptidase activity hydrolase activity peptide binding identical protein binding protein homodimerization activity serine protease

Process(es)

behavioral fear response response to hypoxia regulation of T cell mediated immunity proteolysis cell adhesion positive regulation of cell proliferation negative regulation of extracellular matrix disassembly T cell costimulation regulation of cell-cell adhesion mediated by integrin locomotory exploration behavior psychomotor behavior T cell activation endothelial cell migration viral entry into host cell establishment of localization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD26 Monoclonal Antibody (H194-112 (H194-112.12.4.4.5)), PerCP-Cyanine5.5, eBioscience™

CD26 Antibody (45-0261-82) in Flow

Product Details

45-0261-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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