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Invitrogen
CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-1), Super Bright™ 436, eBioscience™
FIGURE: 1 / 1
CD266 (TWEAK Receptor) Antibody (62-9019-42) in Flow
Staining of human CD266-transfected L5178Y cells with Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (blue histogram) or 0.5 µg of CD266 Monoclonal Antibody, Super Bright 436 (purple histogram). Total viable cells were analyzed.
Product Details
62-9019-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ITEM-1
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762761
Product Specific Information
Description: The ITEM-1 mAb reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration.
Applications Reported: This ITEM-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This ITEM-1 antibody has been pre-diluted and tested by flow cytometric analysis. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis in demonstrate that this relatively small molecule is a fully transformed cell lines. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library. TweakR is a small protein belonging to TNF receptor super family. TweakR is capable of intiating a proliferative signal by binding to TRAF1, 2, and 3. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver. It may also play a role in vascular cell migration and angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; Tweak-receptor; TweakR; type I transmembrane protein Fn14
Gene Aliases: CD266; FN14; TNFRSF12A; TWEAKR
UniProt ID: (Human) Q9NP84
Entrez Gene ID: (Human) 51330
angiogenesis
substrate-dependent cell migration, cell attachment to substrate
tumor necrosis factor-mediated signaling pathway
positive regulation of apoptotic process
regulation of angiogenesis
positive regulation of axon extension
regulation of wound healing
extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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