Invitrogen

CD268 (BAFF Receptor) Monoclonal Antibody (eBio7H22-E16), FITC, eBioscience™

3 Published Figures

10 References

View all (31) BAFF Receptor (CD268) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

CD268 (BAFF Receptor) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD268 (BAFF Receptor) Antibody (11-5943-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 PE (Product # 12-0193-82) and 0.125 µg of FITC Rat IgG1 kappa Isotype Control (Product # 11-4301-82) (left) or 0.125 µg of Anti-Mouse CD268 (BAFF Receptor) FITC (right). Cells in the lymphocyte gate were used for analysis.

CD268 (BAFF Receptor) Antibody in T-Cell Activation (TCA)

Product Image Gallery

Product Details

11-5943-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 9 publications 9 publications

T-Cell Activation (TCA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio7H22-E16

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837079

Product Specific Information

Description: The monoclonal antibody eBio7H22.E16 reacts with mouse BAFF-Receptor, a type III transmembrane TNF receptor family member. BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice. The receptor is found on the surface of all B220+ B cells found in spleen. BAFF-R is expressed on activated/memory subsets of T-cells and is important for splenic B cell maturation and survival and is a major mediator of BAFF- dependent costimulatory responses in peripheral B and T cells. BAFF-R can cause a high level of autoimmune disease and a defect in BAFF-R will cause a decrease in generating mature B-Cells The ligand for the BAFF-R is BAFF also known as BLyS.

Applications Reported: This eBio7H22-E16 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio7H22-E16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13. 1-q13. 31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD11b^high B Cells Increase after Stroke and Regulate Microglia.

Journal of immunology (Baltimore, Md. : 1950)

Korf JM,Honarpisheh P,Mohan EC,Banerjee A,Blasco-Conesa MP,Honarpisheh P,Guzman GU,Khan R,Ganesh BP,Hazen AL,Lee J,Kumar A,McCullough LD,Chauhan A

11-5943-82 was used in Flow cytometry/Cell sorting to investigate the role of CD11bhigh B cells in immune responses after stroke in young and aged mice.

Fri Jul 15 00:00:00 EDT 2022

Essential requirement for polypyrimidine tract binding proteins 1 and 3 in the maturation and maintenance of mature B cells in mice.

European journal of immunology

Monzón-Casanova E,Bates KJ,Smith CWJ,Turner M

11-5943-82 was used in Flow Cytometry to show that the polypyrimidine tract binding proteins (PTBP) PTBP1 and PTBP3 bind to a large and overlapping set of transcripts in B cells.

Wed Sep 01 00:00:00 EDT 2021

Essential requirement for polypyrimidine tract binding proteins 1 and 3 in the maturation and maintenance of mature B cells in mice.

European journal of immunology

Monzón-Casanova E,Bates KJ,Smith CWJ,Turner M

11-5943-82 was used in Flow Cytometry to show that the polypyrimidine tract binding proteins (PTBP) PTBP1 and PTBP3 bind to a large and overlapping set of transcripts in B cells.

Wed Sep 01 00:00:00 EDT 2021

S100A9 Derived From Myeloma Associated Myeloid Cells Promotes TNFSF13B/TNFRSF13B-Dependent Proliferation and Survival of Myeloma Cells.

Frontiers in oncology

Meng L,Tang Q,Zhao J,Wang Z,Wei L,Wei Q,Yin L,Luo S,Song J

11-5943-82 was used in Flow Cytometry to examine the effects of myeloid cells on bone marrow cells of multiple myeloma patients by scRNA-seq transcriptome analysis and reveal a high-resolution gene profile of myeloma cells and myeloma-associated myeloid cells.

Tue Jun 22 00:00:00 EDT 2021

MRL Strains Have a BAFFR Mutation without Functional Consequence.

PloS one

Allman WR,Liu L,Coleman AS,Akkoyunlu M

11-5943 was used in Flow cytometry/Cell sorting to conclude that P44S B cell activating factor receptor mutation does not hinder its function or enhance B cell activity in autoimmune mice.

Mon Jul 10 00:00:00 EDT 2017

BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival.

The Journal of experimental medicine

Jacque E,Schweighoffer E,Tybulewicz VL,Ley SC

11-5943 was used in Flow cytometry/Cell sorting to reveal a potentially critical role for the MEK5-ERK5 MAP kinase signalling pathway in BAFF-induced mature B cell survival and homeostatic maintenance of B2 cell numbers.

Mon Jun 01 00:00:00 EDT 2015

Aberrant germinal center formation, follicular T-helper cells, and germinal center B-cells were involved in chronic graft-versus-host disease.

Annals of hematology

Shao L,Lie AK,Zhang Y,Wong CH,Kwong YL

11594382 was used in flow cytometry to explore the roles of T and B cells in chronic graft-versus-host disease

Tue Sep 01 00:00:00 EDT 2015

B cell activating factor inhibition impairs bacterial immunity by reducing T cell-independent IgM secretion.

Infection and immunity

Jones DD,Jones M,DeIulio GA,Racine R,MacNamara KC,Winslow GM

11-5943 was used in Flow cytometry/Cell sorting to determine that BAFF signalling can directly regulate secretory processes.

Sun Dec 01 00:00:00 EST 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

11-5943 was used in Flow cytometry/Cell sorting to demonstrate the essential role of Bcl10 in ensuring efficacy of BAFF, responsible for regulating NF-κB activation and subsequent survival of anergic B cells.

Sat Dec 01 00:00:00 EST 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Sat Dec 01 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

11-5943 was used in Flow cytometry/Cell sorting to demonstrate that nuclear export of IκBα is essential in maintaining constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.

Fri Feb 25 00:00:00 EST 2011

Bioinformatics

Protein Aliases: b cell-activating factor receptor; B-cell maturation defect; B-cell maturation defect 1; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD268; Tumor necrosis factor receptor superfamily member 13C

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Gene Aliases: 2010006P15Rik; BAFF-R; Baffr; Bcmd; Bcmd-1; Bcmd1; Br3; Lvis22; Tnfrsf13c

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UniProt ID: (Mouse) Q9D8D0

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Entrez Gene ID: (Mouse) 72049

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Function(s)

transmembrane signal receptor

Process(es)

B cell homeostasis immune system process positive regulation of germinal center formation positive regulation of B cell proliferation T cell costimulation B cell costimulation positive regulation of T cell proliferation positive regulation of interferon-gamma biosynthetic process regulation of immune response

Performance Guarantee

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CD268 (BAFF Receptor) Monoclonal Antibody (eBio7H22-E16), FITC, eBioscience™

CD268 (BAFF Receptor) Antibody (11-5943-82) in Flow

Product Details

11-5943-82

Flow Cytometry (Flow)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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