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Invitrogen
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Brilliant Violet™ 786, eBioscience™
FIGURE: 1 / 1
CD274 (PD-L1, B7-H1) Antibody (417-5983-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated overnight with PHA-L (Product # 00-4977-03) (right). Cells were then stained with Mouse IgG1 kappa Isotype Control, Brilliant Violet 786 (Product # 417-4714-81) (blue histogram) or CD274 (PD-L1, B7-H1) Monoclonal Antibody, Brilliant Violet 786 (purple histogram). Viable cells in the lymphocyte gate were used for analysis, as determin... View More
Product Details
417-5983-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIH1
Conjugate
Excitation/Emission Max
406/788 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Brilliant Violet™ 786 (BV786) is a tandem dye that emits at 786 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Our internal testing suggests that Brilliant Violet™ 786 (BV786) is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Aliases: B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1
UniProt ID: (Human) Q9NZQ7
Entrez Gene ID: (Human) 29126
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell migration
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
response to cytokine
positive regulation of T cell proliferation
regulation of activated T cell proliferation
negative regulation of activated T cell proliferation
regulation of T cell apoptotic process
toxin transport
negative regulation of tumor necrosis factor superfamily cytokine production
positive regulation of interleukin-10 secretion
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