Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor™ 488, eBioscience™

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 40

CD274 (PD-L1, B7-H1) Antibody (53-5983-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated for 72 hours with PHA-L (Product # 00-4977-03) (right). Cells were then stained with Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (blue histogram) or CD274 Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Viable cells in the lymphocyte gate were used for analysis. Viable cells were determined by 7-... View More

CD274 (PD-L1, B7-H1) Antibody in Immunocytochemistry (ICC/IF)

CD274 (PD-L1, B7-H1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD274 (PD-L1, B7-H1) Antibody in Immunohistochemistry (IHC)

CD274 (PD-L1, B7-H1) Antibody in Western Blot (WB)

CD274 (PD-L1, B7-H1) Antibody in Neutralization (Neu)

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Product Details

53-5983-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 24 publications 24 publications

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802335

Product Specific Information

Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.

Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH1 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Breast Cancer Are Regulated by N-Linked Glycosylation-Dependent Activation of STAT3 and STAT1.

Cells

Erlichman N,Meshel T,Baram T,Abu Raiya A,Horvitz T,Ben-Yaakov H,Ben-Baruch A

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Sat Sep 23 00:00:00 EDT 2023

NKp44-Derived Peptide Used in Combination Stimulates Antineoplastic Efficacy of Targeted Therapeutic Drugs.

International journal of molecular sciences

Iraqi M,Bolel P,Sarkar R,Bhattacharya B,Abu Ahmad M,Edri A,Roisman LC,Elkabets M,Shalata W,Peled N,Porgador A

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2022

FOXM1 Inhibition Enhances the Therapeutic Outcome of Lung Cancer Immunotherapy by Modulating PD-L1 Expression and Cell Proliferation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Madhi H,Lee JS,Choi YE,Li Y,Kim MH,Choi Y,Goh SH

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Immunocytochemistry

Sat Oct 01 00:00:00 EDT 2022

GM-CSF, Flt3-L and IL-4 affect viability and function of conventional dendritic cell types 1 and 2.

Frontiers in immunology

Lellahi SM,Azeem W,Hua Y,Gabriel B,Paulsen Rye K,Reikvam H,Kalland KH

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

PD-L1 CAR effector cells induce self-amplifying cytotoxic effects against target cells.

Journal for immunotherapy of cancer

Bajor M,Graczyk-Jarzynka A,Marhelava K,Burdzinska A,Muchowicz A,Goral A,Zhylko A,Soroczynska K,Retecki K,Krawczyk M,Klopotowska M,Pilch Z,Paczek L,Malmberg KJ,Wälchli S,Winiarska M,Zagozdzon R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

PD-L1 CAR effector cells induce self-amplifying cytotoxic effects against target cells.

Journal for immunotherapy of cancer

Bajor M,Graczyk-Jarzynka A,Marhelava K,Burdzinska A,Muchowicz A,Goral A,Zhylko A,Soroczynska K,Retecki K,Krawczyk M,Klopotowska M,Pilch Z,Paczek L,Malmberg KJ,Wälchli S,Winiarska M,Zagozdzon R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Ruxolitinib reverses checkpoint inhibition by reducing programmed cell death ligand-1 (PD-L1) expression and increases anti-tumour effects of T cells in multiple myeloma.

British journal of haematology

Chen H,Li M,Ng N,Yu E,Bujarski S,Yin Z,Wen M,Hekmati T,Field D,Wang J,Nassir I,Yu J,Huang J,Daniely D,Wang CS,Xu N,Spektor TM,Berenson JR

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

Protecting Stem Cell Derived Pancreatic Beta-Like Cells From Diabetogenic T Cell Recognition.

Frontiers in endocrinology

Castro-Gutierrez R,Alkanani A,Mathews CE,Michels A,Russ HA

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Thu Feb 10 00:00:00 EST 2022

IL-27 Mediates PD-L1 Expression and Release by Human Mesothelioma Cells.

Cancers

Carbotti G,Dozin B,Martini S,Giordano C,Scordamaglia F,Croce M,Filaci G,Ferrini S,Fabbi M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Mon Aug 09 00:00:00 EDT 2021

Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer.

Biomedicines

Bamodu OA,Wang YH,Yeh CT,Ho CH,Chiang YT,Kao WT,Liu CH,Wu CC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.

Nature communications

Zhang X,Huang X,Xu J,Li E,Lao M,Tang T,Zhang G,Guo C,Zhang X,Chen W,Yadav DK,Bai X,Liang T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Immunocytochemistry

Tue Jul 27 00:00:00 EDT 2021

Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.

STAR protocols

Spangenberg SH,Zavareh RB,Lairson LL

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Microbe-Mediated Activation of Toll-like Receptor 2 Drives PDL1 Expression in HNSCC.

Cancers

Mann JE,Ludwig ML,Kulkarni A,Scheftz EB,Murray IR,Zhai J,Gensterblum-Miller E,Jiang H,Brenner JC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Western Blot

Fri Sep 24 00:00:00 EDT 2021

X-linked recessive TLR7 deficiency in ~1% of men under 60 years old with life-threatening COVID-19.

Science immunology

Asano T,Boisson B,Onodi F,Matuozzo D,Moncada-Velez M,Maglorius Renkilaraj MRL,Zhang P,Meertens L,Bolze A,Materna M,Korniotis S,Gervais A,Talouarn E,Bigio B,Seeleuthner Y,Bilguvar K,Zhang Y,Neehus AL,Ogishi M,Pelham SJ,Le Voyer T,Rosain J,Philippot Q,Soler-Palacín P,Colobran R,Martin-Nalda A,Rivière JG,Tandjaoui-Lambiotte Y,Chaïbi K,Shahrooei M,Darazam IA,Olyaei NA,Mansouri D,Hatipoğlu N,Palabiyik F,Ozcelik T,Novelli G,Novelli A,Casari G,Aiuti A,Carrera P,Bondesan S,Barzaghi F,Rovere-Querini P,Tresoldi C,Franco JL,Rojas J,Reyes LF,Bustos IG,Arias AA,Morelle G,Christèle K,Troya J,Planas-Serra L,Schlüter A,Gut M,Pujol A,Allende LM,Rodriguez-Gallego C,Flores C,Cabrera-Marante O,Pleguezuelo DE,de Diego RP,Keles S,Aytekin G,Akcan OM,Bryceson YT,Bergman P,Brodin P,Smole D,Smith CIE,Norlin AC,Campbell TM,Covill LE,Hammarström L,Pan-Hammarström Q,Abolhassani H,Mane S,Marr N,Ata M,Al Ali F,Khan T,Spaan AN,Dalgard CL,Bonfanti P,Biondi A,Tubiana S,Burdet C,Nussbaum R,Kahn-Kirby A,Snow AL,Bustamante J,Puel A,Boisson-Dupuis S,Zhang SY,Béziat V,Lifton RP,Bastard P,Notarangelo LD,Abel L,Su HC,Jouanguy E,Amara A,Soumelis V,Cobat A,Zhang Q,Casanova JL

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Thu Aug 19 00:00:00 EDT 2021

EGCG Inhibits Tumor Growth in Melanoma by Targeting JAK-STAT Signaling and Its Downstream PD-L1/PD-L2-PD1 Axis in Tumors and Enhancing Cytotoxic T-Cell Responses.

Pharmaceuticals (Basel, Switzerland)

Ravindran Menon D,Li Y,Yamauchi T,Osborne DG,Vaddi PK,Wempe MF,Zhai Z,Fujita M

53-5983-42 was used in Flow cytometry/Cell sorting to demonstrate that EGCG enhances anti-tumor immune responses by inhibiting JAK-STAT signaling in melanoma.

Tue Oct 26 00:00:00 EDT 2021

TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9.

Nature

Heinz LX,Lee J,Kapoor U,Kartnig F,Sedlyarov V,Papakostas K,César-Razquin A,Essletzbichler P,Goldmann U,Stefanovic A,Bigenzahn JW,Scorzoni S,Pizzagalli MD,Bensimon A,Müller AC,King FJ,Li J,Girardi E,Mbow ML,Whitehurst CE,Rebsamen M,Superti-Furga G

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

β-Catenin induces transcriptional expression of PD-L1 to promote glioblastoma immune evasion.

The Journal of experimental medicine

Du L,Lee JH,Jiang H,Wang C,Wang S,Zheng Z,Shao F,Xu D,Xia Y,Li J,Zheng Y,Qian X,Li X,Kim HR,Xing D,Liu P,Lu Z,Lyu J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2020

Protein kinase D3 regulates the expression of the immunosuppressive protein, PD‑L1, through STAT1/STAT3 signaling.

International journal of oncology

Cui B,Chen J,Luo M,Wang L,Chen H,Kang Y,Wang J,Zhou X,Feng Y,Zhang P

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis.

Nature communications

Oulès B,Philippeos C,Segal J,Tihy M,Vietri Rudan M,Cujba AM,Grange PA,Quist S,Natsuga K,Deschamps L,Dupin N,Donati G,Watt FM

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Phospho‑STAT1 expression as a potential biomarker for anti‑PD‑1/anti‑PD‑L1 immunotherapy for breast cancer.

International journal of oncology

Nakayama Y,Mimura K,Tamaki T,Shiraishi K,Kua LF,Koh V,Ohmori M,Kimura A,Inoue S,Okayama H,Suzuki Y,Nakazawa T,Ichikawa D,Kono K

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy.

Cell reports

Celis-Gutierrez J,Blattmann P,Zhai Y,Jarmuzynski N,Ruminski K,Grégoire C,Ounoughene Y,Fiore F,Aebersold R,Roncagalli R,Gstaiger M,Malissen B

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

High PD-L1 Expression Predicts for Worse Outcome of Leukemia Patients with Concomitant NPM1 and FLT3 Mutations.

International journal of molecular sciences

Brodská B,Otevřelová P,Šálek C,Fuchs O,Gašová Z,Kuželová K

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Mon Jun 10 00:00:00 EDT 2019

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids.

Cell

Dijkstra KK,Cattaneo CM,Weeber F,Chalabi M,van de Haar J,Fanchi LF,Slagter M,van der Velden DL,Kaing S,Kelderman S,van Rooij N,van Leerdam ME,Depla A,Smit EF,Hartemink KJ,de Groot R,Wolkers MC,Sachs N,Snaebjornsson P,Monkhorst K,Haanen J,Clevers H,Schumacher TN,Voest EE

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Specific expression of PD-L1 in RELA-fusion supratentorial ependymoma: Implications for PD-1-targeted therapy.

Pediatric blood & cancer

Witt DA,Donson AM,Amani V,Moreira DC,Sanford B,Hoffman LM,Handler MH,Levy JMM,Jones KL,Nellan A,Foreman NK,Griesinger AM

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

A versatile T cell-based assay to assess therapeutic antigen-specific PD-1-targeted approaches.

Oncotarget

Versteven M,Van den Bergh JMJ,Broos K,Fujiki F,Campillo-Davo D,De Reu H,Morimoto S,Lecocq Q,Keyaerts M,Berneman Z,Sugiyama H,Van Tendeloo VFI,Breckpot K,Lion E

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Neutralization

Tue Jun 12 00:00:00 EDT 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Apigenin suppresses PD-L1 expression in melanoma and host dendritic cells to elicit synergistic therapeutic effects.

Journal of experimental & clinical cancer research : CR

Xu L,Zhang Y,Tian K,Chen X,Zhang R,Mu X,Wu Y,Wang D,Wang S,Liu F,Wang T,Zhang J,Liu S,Zhang Y,Tu C,Liu H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Mon Oct 29 00:00:00 EDT 2018

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Immunocytochemistry

Fri Dec 15 00:00:00 EST 2017

Upregulation of Programmed Death-1 and Its Ligand in Cardiac Injury Models: Interaction with GADD153.

PloS one

Baban B,Liu JY,Qin X,Weintraub NL,Mozaffari MS

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Neutralization

Tue Apr 12 00:00:00 EDT 2016

Alterations of immune response of Non-Small Cell Lung Cancer with Azacytidine.

Oncotarget

Wrangle J,Wang W,Koch A,Easwaran H,Mohammad HP,Vendetti F,Vancriekinge W,Demeyer T,Du Z,Parsana P,Rodgers K,Yen RW,Zahnow CA,Taube JM,Brahmer JR,Tykodi SS,Easton K,Carvajal RD,Jones PA,Laird PW,Weisenberger DJ,Tsai S,Juergens RA,Topalian SL,Rudin CM,Brock MV,Pardoll D,Baylin SB

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 53-5983-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

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Gene Aliases: B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1

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UniProt ID: (Human) Q9NZQ7

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Entrez Gene ID: (Human) 29126

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production response to cytokine positive regulation of T cell proliferation regulation of activated T cell proliferation negative regulation of activated T cell proliferation regulation of T cell apoptotic process toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor™ 488, eBioscience™

CD274 (PD-L1, B7-H1) Antibody (53-5983-42) in Flow

Product Details

53-5983-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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