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CD275 (B7-H2) Antibody (PA5-47161) in ICC/IF
Immunocytochemistry analysis of CD275 (B7-H2) in immersion fixed mouse splenocytes. Samples were incubated in CD275 (B7-H2) polyclonal antibody (Product # PA5-47161) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47161
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2 Glu47-Lys279
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2577162
Product Specific Information
In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) B7-H2, recombinant mouse (rm) B7-1, rhB7-1, and rmB7-2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; LICOS
Gene Aliases: AU044799; B7-H2; B7h; B7h2; B7RP-1; B7rp1; BG071784; GI50; GL50; GL50-B; ICOS-L; Icosl; Icoslg; LICOS; Ly115l; mKIAA0653
UniProt ID: (Mouse) Q9JHJ8
Entrez Gene ID: (Mouse) 50723
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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