Invitrogen

CD275 (B7-H2) Monoclonal Antibody (MIH12), PE, eBioscience™

CD275 (B7-H2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD275 (B7-H2) Antibody (12-5889-42) in Flow

Staining of normal human peripheral blood monocyte-derived dendritic cells with Mouse IgG1 K Isotype Control PE (open histogram) (Product # 12-4714-81) or Anti-Human CD275 (B7-H2) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-5889-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 12 publications 12 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853668

Product Specific Information

Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.

Applications Reported: The MIH12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

12-5889-42 was used in Flow cytometry/Cell sorting to report that human blood CD1c+ dendritic cells activated by GM-CSF (also known as CSF2) drive the differentiation of naive CD4+ T cells into Tfh1 cells.

Tue Nov 01 00:00:00 EDT 2022

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Tue Nov 01 00:00:00 EDT 2022

Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion.

eLife

Angulo G,Zeleznjak J,Martínez-Vicente P,Puñet-Ortiz J,Hengel H,Messerle M,Oxenius A,Jonjic S,Krmpotić A,Engel P,Angulo A

Published figure using CD275 (B7-H2) monoclonal antibody (Product # 12-5889-42) in Flow Cytometry

Mon Jan 18 00:00:00 EST 2021

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

12-5889 was used in Western Blotting to study how besides SOX2 expression, no other pluripotency features were observed in early and late passages of CV-MSC.

Mon Feb 05 00:00:00 EST 2018

Targeting the Human T-Cell Inducible COStimulator Molecule with a Monoclonal Antibody Prevents Graft-vs-Host Disease and Preserves Graft vs Leukemia in a Xenograft Murine Model.

Frontiers in immunology

Burlion A,Brunel S,Petit NY,Olive D,Marodon G

12-5889 was used in Flow cytometry/Cell sorting to determine the role of monoclonal antibody to human ICOS in costimulatory signalling to human T cells.

Wed Nov 20 00:00:00 EST 2019

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using CD275 (B7-H2) monoclonal antibody (Product # 12-5889-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.

Oncoimmunology

Pedroza-Gonzalez A,Zhou G,Vargas-Mendez E,Boor PP,Mancham S,Verhoef C,Polak WG,Grünhagen D,Pan Q,Janssen H,Garcia-Romo GS,Biermann K,Tjwa ET,IJzermans JN,Kwekkeboom J,Sprengers D

Published figure using CD275 (B7-H2) monoclonal antibody (Product # 12-5889-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

A novel subset of B7-H3⁺CD14⁺HLA-DR^-/low myeloid-derived suppressor cells are associated with progression of human NSCLC.

Oncoimmunology

Zhang G,Huang H,Zhu Y,Yu G,Gao X,Xu Y,Liu C,Hou J,Zhang X

12-5889 was used in Flow cytometry/Cell sorting to assess the contribution of myeloid-derived suppressor cells to tumour progression and metastasis.

Sun Feb 01 00:00:00 EST 2015

Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity.

Nature communications

Willmann KL,Klaver S,Doğu F,Santos-Valente E,Garncarz W,Bilic I,Mace E,Salzer E,Conde CD,Sic H,Májek P,Banerjee PP,Vladimer GI,Haskoloğlu S,Bolkent MG,Küpesiz A,Condino-Neto A,Colinge J,Superti-Furga G,Pickl WF,van Zelm MC,Eibel H,Orange JS,Ikincioğulları A,Boztuğ K

12-5889 was used in Flow cytometry/Cell sorting to study the effect of a biallelic mutation in the gene encoding NF-κB-inducing kinase on immune cell populations.

Wed Nov 19 00:00:00 EST 2014

CagA-dependent downregulation of B7-H2 expression on gastric mucosa and inhibition of Th17 responses during Helicobacter pylori infection.

Journal of immunology (Baltimore, Md. : 1950)

Lina TT,Pinchuk IV,House J,Yamaoka Y,Graham DY,Beswick EJ,Reyes VE

12-5889 was used in Flow cytometry/Cell sorting to investigate the impact of H.pylori CagA on the modulation of the expression of the T cell costimulator B7-H2 by gastric epithelial cells.

Tue Oct 01 00:00:00 EDT 2013

Novel role of plasmacytoid dendritic cells in humans: induction of interleukin-10-producing Treg cells by plasmacytoid dendritic cells in patients with rheumatoid arthritis responding to therapy.

Arthritis and rheumatism

Kavousanaki M,Makrigiannakis A,Boumpas D,Verginis P

12-5889 was used in Flow cytometry/Cell sorting to explore the contribution of dendritic cells to the development of regulatory T cells in a human autoimmune disease setting.

Fri Jan 01 00:00:00 EST 2010

Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.

Scandinavian journal of immunology

Youngnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M

Wed Mar 01 00:00:00 EST 2006

Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.

Scandinavian journal of immunology

Youngnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M

Wed Mar 01 00:00:00 EST 2006

Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.

Scandinavian journal of immunology

Youngnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M

Wed Mar 01 00:00:00 EST 2006

Expression and function of inducible costimulator on peripheral blood T cells in patients with systemic lupus erythematosus.

Rheumatology (Oxford, England)

Yang JH,Zhang J,Cai Q,Zhao DB,Wang J,Guo PE,Liu L,Han XH,Shen Q

12-5889 was used in Flow cytometry/Cell sorting to show that the abnormality of ICOS costimulation in SLE patients may play an immunopathological role in pathogenesis.

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: B7 homolog 2; B7 homologue 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; inducible T-cell co-stimulator ligand; transmembrane protein B7-H2 ICOS ligand

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Gene Aliases: B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; ICOSLG; KIAA0653; LICOS

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UniProt ID: (Human) O75144

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Entrez Gene ID: (Human) 23308

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Function(s)

receptor binding immunoglobulin receptor superfamily

Process(es)

adaptive immune response defense response hyperosmotic response signal transduction T cell costimulation positive regulation of activated T cell proliferation T cell activation B cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD275 (B7-H2) Monoclonal Antibody (MIH12), PE, eBioscience™

CD275 (B7-H2) Antibody (12-5889-42) in Flow

Product Details

12-5889-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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