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Invitrogen
CD276 (B7-H3) Monoclonal Antibody (MIH35), Functional Grade, eBioscience™
FIGURE: 1 / 2
CD276 (B7-H3) Antibody (16-5937-81) in Flow
Staining of B7-H3-transfected cells with 0.25 µg of Rat IgG2a Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.5 µg of Anti-Human/Mouse CD276 (B7-H3) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG PE (Product # 12-4822).Total viable cells were used for analysis.
Product Details
16-5937-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIH35
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1548799
Product Specific Information
Description: This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2).
Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis.
Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276; CD276 antigen; Costimulatory molecule; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276
Gene Aliases: 4Ig-B7-H3; 6030411F23Rik; AU016588; B7-H3; B7H3; B7RP-2; CD276; PSEC0249; UNQ309/PRO352
UniProt ID: (Human) Q5ZPR3, (Mouse) Q8VE98
Entrez Gene ID: (Human) 80381, (Mouse) 102657
immune response
cell proliferation
positive regulation of bone mineralization
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
negative regulation of interferon-gamma biosynthetic process
positive regulation of interferon-gamma biosynthetic process
negative regulation of interleukin-2 biosynthetic process
positive regulation of osteoblast differentiation
negative regulation of inflammatory response
regulation of immune response
positive regulation of interleukin-2 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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