Invitrogen

CD278 (ICOS) Monoclonal Antibody (ISA-3), Biotin, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (13-9948-82) in Flow

Staining of 2-day unstimulated (left) and 2-day Anti-Human CD3/CD28 (Product # 16-0039-81) and (Product # 16-0289-81) activated (right) PBMC with Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (open histogram) or Anti-Human CD278 (ICOS) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Viable cells were analyzed.

CD278 (ICOS) Antibody in Neutralization (Neu)

CD278 (ICOS) Antibody in T-Cell Activation (TCA)

Product Image Gallery

Product Details

13-9948-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 17 publications 17 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ISA-3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467004

Product Specific Information

Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (Product # 14-9949-82) , was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use Product # 13-9948-82 or Product # 12-9948-42 rather than Product # 11-9948-42 .

Applications Reported: The ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The ISA-3 antibody has been tested by flow cytometric analysis of unstimulated and CD3/CD28-stimulated (3 days) human blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inducible Costimulator-C-X-C Motif Chemokine Receptor 3 Signaling is Involved in Chronic Obstructive Pulmonary Disease Pathogenesis.

International journal of chronic obstructive pulmonary disease

Li DY,Chen L,Miao SY,Zhou M,Wu JH,Sun SW,Liu LL,Qi C,Xiong XZ

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Neutralization

Tue Aug 23 00:00:00 EDT 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Reduced blood-stage malaria growth and immune correlates in humans following RH5 vaccination.

Med (New York, N.Y.)

Minassian AM,Silk SE,Barrett JR,Nielsen CM,Miura K,Diouf A,Loos C,Fallon JK,Michell AR,White MT,Edwards NJ,Poulton ID,Mitton CH,Payne RO,Marks M,Maxwell-Scott H,Querol-Rubiera A,Bisnauthsing K,Batra R,Ogrina T,Brendish NJ,Themistocleous Y,Rawlinson TA,Ellis KJ,Quinkert D,Baker M,Lopez Ramon R,Ramos Lopez F,Barfod L,Folegatti PM,Silman D,Datoo M,Taylor IJ,Jin J,Pulido D,Douglas AD,de Jongh WA,Smith R,Berrie E,Noe AR,Diggs CL,Soisson LA,Ashfield R,Faust SN,Goodman AL,Lawrie AM,Nugent FL,Alter G,Long CA,Draper SJ

13-9948-82 was used in Flow Cytometry to assess the safety, immunogenicity, and efficacy against blood-stage CHMI of the RH5.1 recombinant protein vaccine.

Fri Jun 11 00:00:00 EDT 2021

Protein/AS01_B vaccination elicits stronger, more Th2-skewed antigen-specific human T follicular helper cell responses than heterologous viral vectors.

Cell reports. Medicine

Nielsen CM,Ogbe A,Pedroza-Pacheco I,Doeleman SE,Chen Y,Silk SE,Barrett JR,Elias SC,Miura K,Diouf A,Bardelli M,Dabbs RA,Barfod L,Long CA,Haynes BF,Payne RO,Minassian AM,Bradley T,Draper SJ,Borrow P

13-9948-82 was used in Flow Cytometry to highlight the impact of vaccine platform on the cTfh cell response driving humoral immunity, associating a high-magnitude, Th2-biased cTfh response with potent antibody production.

Tue Mar 16 00:00:00 EDT 2021

Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.

Scientific reports

Dart SJ,Cook AM,Millward MJ,McDonnell AM,Chin WL,Hakeem MU,Meniawy TM,Bowyer SE

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Frontiers in immunology

Khanam A,Ayithan N,Tang L,Poonia B,Kottilil S

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Wed Jun 16 00:00:00 EDT 2021

The adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes.

The Journal of experimental medicine

Hill DL,Pierson W,Bolland DJ,Mkindi C,Carr EJ,Wang J,Houard S,Wingett SW,Audran R,Wallin EF,Jongo SA,Kamaka K,Zand M,Spertini F,Daubenberger C,Corcoran AE,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Mon Aug 05 00:00:00 EDT 2019

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Wed Sep 19 00:00:00 EDT 2018

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Polyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS.

Oncoimmunology

Franzese O,Palermo B,Di Donna C,Sperduti I,Ferraresi V,Stabile H,Gismondi A,Santoni A,Nisticò P

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in T-Cell Activation

Sun May 01 00:00:00 EDT 2016

Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.

Oncoimmunology

Pedroza-Gonzalez A,Zhou G,Vargas-Mendez E,Boor PP,Mancham S,Verhoef C,Polak WG,Grünhagen D,Pan Q,Janssen H,Garcia-Romo GS,Biermann K,Tjwa ET,IJzermans JN,Kwekkeboom J,Sprengers D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9948-82) in T-Cell Activation

Mon Jun 01 00:00:00 EDT 2015

Phase I study of the CD40 agonist antibody CP-870,893 combined with carboplatin and paclitaxel in patients with advanced solid tumors.

Oncoimmunology

Vonderheide RH,Burg JM,Mick R,Trosko JA,Li D,Shaik MN,Tolcher AW,Hamid O

13-9948 was used in Flow cytometry/Cell sorting to assess the safety of a human CD40-targeting agonist mAb administered intravenously in combination with two chemotherapy drugs in patients with advanced solid tumours.

Tue Jan 01 00:00:00 EST 2013

Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.

Proceedings of the National Academy of Sciences of the United States of America

Bentebibel SE,Schmitt N,Banchereau J,Ueno H

13-9948 was used in Flow cytometry/Cell sorting to identify and investigate the role of a previously undefined BCL6-expressing CD4(+) T-cell subset in human tonsils.

Tue Aug 16 00:00:00 EDT 2011

Preoperative CTLA-4 blockade: tolerability and immune monitoring in the setting of a presurgical clinical trial.

Clinical cancer research : an official journal of the American Association for Cancer Research

Carthon BC,Wolchok JD,Yuan J,Kamat A,Ng Tang DS,Sun J,Ku G,Troncoso P,Logothetis CJ,Allison JP,Sharma P

13-9948 was used in Flow cytometry/Cell sorting to demonstrate that anti-CTLA-4 therapy has a tolerable safety profile in the pre-surgical setting.

Sat May 15 00:00:00 EDT 2010

Human naive and memory CD4+ T cell repertoires specific for naturally processed antigens analyzed using libraries of amplified T cells.

The Journal of experimental medicine

Geiger R,Duhen T,Lanzavecchia A,Sallusto F

13-9948 was used in Flow cytometry/Cell sorting to determine the frequency and antigen dose-response of human naive CD4(+) T cells specific for a variety of antigens and to isolate antigen-specific T cell clones.

Mon Jul 06 00:00:00 EDT 2009

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

13-9948 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Quiroga MF,Pasquinelli V,Martínez GJ,Jurado JO,Zorrilla LC,Musella RM,Abbate E,Sieling PA,García VE

Mon May 15 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CD278; inducible costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CD278; CVID1; ICOS

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UniProt ID: (Human) Q9Y6W8

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Entrez Gene ID: (Human) 29851

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Process(es)

T cell tolerance induction immune response single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (ISA-3), Biotin, eBioscience™

CD278 (ICOS) Antibody (13-9948-82) in Flow

Product Details

13-9948-82

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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