Invitrogen

CD278 (ICOS) Monoclonal Antibody (ISA-3), APC, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (17-9948-42) in Flow

Staining of 3-day unstimulated (left) or 3-day Anti-Human CD3/CD28-stimulated (Product # 16-0039-81 and Product # 16-0289-81) (right) normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD278 (ICOS) APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-9948-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 18 publications 18 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ISA-3

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598209

Product Specific Information

Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (Product # 14-9949-82) , was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use Product # 13-9948-82 or Product # 12-9948-42 rather than Product # 11-9948-42 .

Applications Reported: This ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Characterization of T Follicular Helper Cells and T Follicular Regulatory Cells in HIV-Infected and Sero-Negative Individuals.

Cells

Salvatore B,Resop RS,Gordon BR,Epeldegui M,Martinez-Maza O,Comin-Anduix B,Lam A,Wu TT,Uittenbogaart CH

17-9948-42 was used in Flow cytometry/Cell sorting to identify cytokines in peripheral blood T follicular helper cells and T follicular regulatory cells.

Thu Jan 12 00:00:00 EST 2023

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

17-9948-42 was used in Flow Cytometry to show increased formation of Tfh precursors (pre-Tfh) but no associated increase in germinal centre (GC)-Tfh cells in aged mice, suggesting age-driven promotion of only early Tfh cell differentiation.

Fri Jan 01 00:00:00 EST 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.

Scientific reports

Dart SJ,Cook AM,Millward MJ,McDonnell AM,Chin WL,Hakeem MU,Meniawy TM,Bowyer SE

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Frontiers in immunology

Khanam A,Ayithan N,Tang L,Poonia B,Kottilil S

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Wed Jun 16 00:00:00 EDT 2021

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

17-9948 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

The adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes.

The Journal of experimental medicine

Hill DL,Pierson W,Bolland DJ,Mkindi C,Carr EJ,Wang J,Houard S,Wingett SW,Audran R,Wallin EF,Jongo SA,Kamaka K,Zand M,Spertini F,Daubenberger C,Corcoran AE,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Mon Aug 05 00:00:00 EDT 2019

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9948-42) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

Azacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects.

Oncoimmunology

Ehx G,Fransolet G,de Leval L,D'Hondt S,Lucas S,Hannon M,Delens L,Dubois S,Drion P,Beguin Y,Humblet-Baron S,Baron F

17-9948 was used in Flow cytometry/Cell sorting to investigate the impact of 5-azacytidine on xenogeneic graft-vs.-host disease and graft-vs.-leukaemia effects in a humanised murine model of transplantation.

Tue Jan 12 00:00:00 EST 2021

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

17-9948 was used in Flow cytometry/Cell sorting to show that Tregs from the inflamed lung of sarcoidosis patients were characterized by a unique ICOS(high) phenotype.

Mon Feb 01 00:00:00 EST 2016

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

17-9948 was used in Flow cytometry/Cell sorting to investigate the expression characteristics and clinical significance of CD28, PD-1, CTLA4 and BTLA on T-cells in patients with chronic HBV and the correlations among these receptors.

Mon Aug 01 00:00:00 EDT 2016

Mycobacterium tuberculosis-Specific IL-21+IFN-γ+CD4+ T Cells Are Regulated by IL-12.

PloS one

Li L,Jiang Y,Lao S,Yang B,Yu S,Zhang Y,Wu C

17-9948 was used in Flow cytometry/Cell sorting to demonstrate that Mycobacterium tuberculosis-specific IL-21+ IFN-γ+- CD4+ T-cells from local sites of tuberculosis infection could be enhanced by IL-12.

Thu Aug 11 00:00:00 EDT 2016

Differential kinetics of human cytomegalovirus load and antibody responses in primary infection of the immunocompetent and immunocompromised host.

The Journal of general virology

Gerna G,Lilleri D,Fornara C,Bruno F,Gabanti E,Cane I,Furione M,Revello MG

17-9948 was used in Flow cytometry/Cell sorting to investigate long-term kinetics of human cytomegalovirus (HCMV) load and HCMV-specific antibody responses in the immunocompetent and immunocompromised solid-organ transplanted host during primary HCMV infection.

Sun Feb 01 00:00:00 EST 2015

Higher Frequency of CD4+CXCR5+ICOS+PD1+ T Follicular Helper Cells in Patients With Infectious Mononucleosis.

Medicine

Liu J,Zhou Y,Yu Q,Zhao Z,Wang H,Luo X,Chen Y,Zhu Z,Chen G,Wu M,Qiu L

17-9948 was used in Flow cytometry/Cell sorting to demonstrate that a CD4CXCR5ICOSPD1 follicular helper T cell subset might play an important role in the pathogenesis of infectious mononucleosis.

Sun Nov 01 00:00:00 EDT 2015

PD-L1hi B cells are critical regulators of humoral immunity.

Nature communications

Khan AR,Hams E,Floudas A,Sparwasser T,Weaver CT,Fallon PG

17-9948 was used in Flow cytometry/Cell sorting to demonstrate that elevated PD-L1 expression on B cells is an important regulator of T follicular helper-cell activity, downstream antibody responses and autoimmune disease.

Thu Jan 22 00:00:00 EST 2015

Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Quiroga MF,Pasquinelli V,Martínez GJ,Jurado JO,Zorrilla LC,Musella RM,Abbate E,Sieling PA,García VE

Mon May 15 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CD278; inducible costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CD278; CVID1; ICOS

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UniProt ID: (Human) Q9Y6W8

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Entrez Gene ID: (Human) 29851

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Process(es)

T cell tolerance induction immune response single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (ISA-3), APC, eBioscience™

CD278 (ICOS) Antibody (17-9948-42) in Flow

Product Details

17-9948-42

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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