Invitrogen

CD278 (ICOS) Monoclonal Antibody (ISA-3), PE-Cyanine7, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (25-9948-42) in Flow

Staining of normal human peripheral blood cells either unstimulated (blue histogram) or stimulated for 3 days with anti-CD3/CD28 (purple histogram) with Anti-Human CD278 (ICOS) PE-Cyanine7. Total viable cells were used for analysis.

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Product Details

25-9948-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 20 publications 20 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ISA-3

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518754

Product Specific Information

Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (Product # 14-9949-82) , was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use Product # 13-9948-82 or Product # 12-9948-42 rather than Product # 11-9948-42 .

Applications Reported: This ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Single-cell characterization of anti-LAG-3 and anti-PD-1 combination treatment in patients with melanoma.

The Journal of clinical investigation

Huuhtanen J,Kasanen H,Peltola K,Lönnberg T,Glumoff V,Brück O,Dufva O,Peltonen K,Vikkula J,Jokinen E,Ilander M,Lee MH,Mäkelä S,Nyakas M,Li B,Hernberg M,Bono P,Lähdesmäki H,Kreutzman A,Mustjoki S

25-9948-42 was used in Flow cytometry/Cell sorting to show that anti-LAG3+anti-PD1 therapy has profound effects on NK cells and Tregs in addition to CD8+ T cells.

Wed Mar 15 00:00:00 EDT 2023

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

25-9948-42 was used in Flow Cytometry to elucidate the molecular mechanisms regulating T follicular helper cells.

Thu Apr 01 00:00:00 EDT 2021

Immunological status of peripheral blood is associated with prognosis in patients with bone and soft-tissue sarcoma.

Oncology letters

Kim Y,Kobayashi E,Suehara Y,Ito A,Kubota D,Tanzawa Y,Endo M,Nakatani F,Nakatsura T,Kawai A,Kaneko K,Kitano S

25-9948 was used in Flow cytometry/Cell sorting to suggest that the immune status of peripheral blood was associated with the prognosis in patients with sarcoma, as previously reported in patients with other cancer types.

Mon Mar 01 00:00:00 EST 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.

Scientific reports

Dart SJ,Cook AM,Millward MJ,McDonnell AM,Chin WL,Hakeem MU,Meniawy TM,Bowyer SE

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Frontiers in immunology

Khanam A,Ayithan N,Tang L,Poonia B,Kottilil S

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Wed Jun 16 00:00:00 EDT 2021

The adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes.

The Journal of experimental medicine

Hill DL,Pierson W,Bolland DJ,Mkindi C,Carr EJ,Wang J,Houard S,Wingett SW,Audran R,Wallin EF,Jongo SA,Kamaka K,Zand M,Spertini F,Daubenberger C,Corcoran AE,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Mon Aug 05 00:00:00 EDT 2019

Ipilimumab plus Lenalidomide after Allogeneic and Autologous Stem Cell Transplantation for Patients with Lymphoid Malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Khouri IF,Fernandez Curbelo I,Turturro F,Jabbour EJ,Milton DR,Bassett RL,Vence LM,Allison JP,Gulbis AM,Sharma P

25-9948 was used in Flow cytometry/Cell sorting to hypothesize that antitumor-cell responses could be enhanced by the addition of lenalidomide to the cytotoxic T-lymphocyte-associated protein 4 inhibitor ipilimumab.

Thu Mar 01 00:00:00 EST 2018

Primary T Cells from Cutaneous T-cell Lymphoma Skin Explants Display an Exhausted Immune Checkpoint Profile.

Cancer immunology research

Querfeld C,Leung S,Myskowski PL,Curran SA,Goldman DA,Heller G,Wu X,Kil SH,Sharma S,Finn KJ,Horwitz S,Moskowitz A,Mehrara B,Rosen ST,Halpern AC,Young JW

25-9948 was used in Flow cytometry/Cell sorting to identify the antigens driving T-cell exhaustion and the evaluation of immune checkpoint inhibition to reverse T-cell exhaustion earlier in treatment of cutaneous T-cell lymphoma.

Wed Aug 01 00:00:00 EDT 2018

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8⁺ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients.

Cancer cell

Naing A,Infante JR,Papadopoulos KP,Chan IH,Shen C,Ratti NP,Rojo B,Autio KA,Wong DJ,Patel MR,Ott PA,Falchook GS,Pant S,Hung A,Pekarek KL,Wu V,Adamow M,McCauley S,Mumm JB,Wong P,Van Vlasselaer P,Leveque J,Tannir NM,Oft M

25-9948-42 was used in Flow cytometry/Cell sorting to report that pegilodecakin induces hallmarks of CD8+ T cell immunity in cancer patients, including elevation of interferon-γ and GranzymeB, expansion and activation of intratumoral CD8+ T cells, and proliferation and expansion of LAG-3+ PD-1+ CD8+ T cells.

Mon Nov 12 00:00:00 EST 2018

Multiparametric characterization of rare HIV-infected cells using an RNA-flow FISH technique.

Nature protocols

Baxter AE,Niessl J,Fromentin R,Richard J,Porichis F,Massanella M,Brassard N,Alsahafi N,Routy JP,Finzi A,Chomont N,Kaufmann DE

25-9948 was used in Flow cytometry/Cell sorting to develop a protocol for flow cytometric identification of viral reservoirs.

Sun Oct 01 00:00:00 EDT 2017

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

Impact of immunosuppressive drugs on the therapeutic efficacy of ex vivo expanded human regulatory T cells.

Haematologica

Scottà C,Fanelli G,Hoong SJ,Romano M,Lamperti EN,Sukthankar M,Guggino G,Fazekasova H,Ratnasothy K,Becker PD,Afzali B,Lechler RI,Lombardi G

25-9948 was used in Flow cytometry/Cell sorting to study the effect of immunosuppressive drugs on Treg cells.

Fri Jan 01 00:00:00 EST 2016

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9948-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation.

European journal of immunology

Cousin C,Aubatin A,Le Gouvello S,Apetoh L,Castellano F,Molinier-Frenkel V

25-9948 was used in Flow cytometry/Cell sorting to study the effect of IL4I1 on naive CD4+ T cell differentiation.

Mon Jun 01 00:00:00 EDT 2015

Distinct and complementary roles for Aspergillus fumigatus-specific Tr1 and Foxp3+ regulatory T cells in humans and mice.

Immunology and cell biology

Bedke T,Iannitti RG,De Luca A,Giovannini G,Fallarino F,Berges C,Latgé JP,Einsele H,Romani L,Topp MS

25-9948 was used in Flow cytometry/Cell sorting to suggest that Tr1 cells most likely play a distinct and complementary role to Foxp3+ regulatory T-cells in antifungal immune homeostasis,.

Mon Sep 01 00:00:00 EDT 2014

Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome.

Blood

Antonelli LR,Mahnke Y,Hodge JN,Porter BO,Barber DL,DerSimonian R,Greenwald JH,Roby G,Mican J,Sher A,Roederer M,Sereti I

25-9948 was used in Flow cytometry/Cell sorting to indicate that IRIS appears to be a predominantly CD4-mediated phenomenon.

Thu Nov 11 00:00:00 EST 2010

Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Quiroga MF,Pasquinelli V,Martínez GJ,Jurado JO,Zorrilla LC,Musella RM,Abbate E,Sieling PA,García VE

Mon May 15 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CD278; inducible costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CD278; CVID1; ICOS

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UniProt ID: (Human) Q9Y6W8

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Entrez Gene ID: (Human) 29851

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Process(es)

T cell tolerance induction immune response single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (ISA-3), PE-Cyanine7, eBioscience™

CD278 (ICOS) Antibody (25-9948-42) in Flow

Product Details

25-9948-42

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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