Invitrogen

CD279 (PD-1) Monoclonal Antibody (MIH4), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

19 Published Figures

31 References

View all (275) PD-1 (CD279) antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

CD279 (PD-1) Antibody (11-9969-42) in Flow

Staining of unstimulated (left) or 3-day PHA-stimulated (right) normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD279 (PD-1) FITC (purple histogram). Total viable cells were used for analysis.

CD279 (PD-1) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-9969-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (2 µg)/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10548937

Product Specific Information

Description: The MIH4 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than antibody clones J105.

Applications Reported: This MIH4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH4 antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. This can be used at 5 µL (2.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Reprogramming of Tumor-reactive Tumor-infiltrating Lymphocytes to Human-induced Pluripotent Stem Cells.

Cancer research communications

Islam SMR,Maeda T,Tamaoki N,Good ML,Kishton RJ,Paria BC,Yu Z,Bosch-Marce M,Bedanova NM,Liu C,Kruhlak MJ,Restifo NP,Vizcardo R

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.

Signal transduction and targeted therapy

Zheng G,Guo Z,Li W,Xi W,Zuo B,Zhang R,Wen W,Yang AG,Jia L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.

Signal transduction and targeted therapy

Zheng G,Guo Z,Li W,Xi W,Zuo B,Zhang R,Wen W,Yang AG,Jia L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Construction of Novel lncRNA-miRNA-mRNA Network Associated With Recurrence and Identification of Immune-Related Potential Regulatory Axis in Hepatocellular Carcinoma.

Frontiers in oncology

Zhan T,Gao X,Wang G,Li F,Shen J,Lu C,Xu L,Li Y,Zhang J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Imaging Changes and Immune-Checkpoint Expression on T Cells in Bronchoalveolar Lavage Fluid from Patients with Pulmonary Sarcoidosis.

Biomedicines

Kotetsu Y,Yanagihara T,Suzuki K,Ando H,Eto D,Hata K,Arimura-Omori M,Yamamoto Y,Harada E,Hamada N

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Thu Sep 16 00:00:00 EDT 2021

Increased Incidence of Venous Thromboembolism with Cancer Immunotherapy.

Med (New York, N.Y.)

Roopkumar J,Swaidani S,Kim AS,Thapa B,Gervaso L,Hobbs BP,Wei W,Alban TJ,Funchain P,Kundu S,Sangwan N,Rayman P,Pavicic PG,Diaz-Montero CM,Barnard J,McCrae KR,Khorana AA

11-9969-42 was used in Flow Cytometry to investigate if venous thromboembolism is an underappreciated and important immune-related adverse event associated with cancer immunotherapy.

Fri Apr 09 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Immunogenic neoantigens derived from gene fusions stimulate T cell responses.

Nature medicine

Yang W,Lee KW,Srivastava RM,Kuo F,Krishna C,Chowell D,Makarov V,Hoen D,Dalin MG,Wexler L,Ghossein R,Katabi N,Nadeem Z,Cohen MA,Tian SK,Robine N,Arora K,Geiger H,Agius P,Bouvier N,Huberman K,Vanness K,Havel JJ,Sims JS,Samstein RM,Mandal R,Tepe J,Ganly I,Ho AL,Riaz N,Wong RJ,Shukla N,Chan TA,Morris LGT

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

According to Hepatitis C Virus (HCV) Infection Stage, Interleukin-7 Plus 4-1BB Triggering Alone or Combined with PD-1 Blockade Increases TRAF1^low HCV-Specific CD8⁺ Cell Reactivity.

Journal of virology

Moreno-Cubero E,Subirá D,Sanz-de-Villalobos E,Parra-Cid T,Madejón A,Miquel J,Olveira A,González-Praetorius A,García-Samaniego J,Larrubia JR

Mon Jan 15 00:00:00 EST 2018

Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells.

Cell reports

Zhao Y,Harrison DL,Song Y,Ji J,Huang J,Hui E

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Tue Jul 10 00:00:00 EDT 2018

Chronic Critical Illness from Sepsis Is Associated with an Enhanced TCR Response.

Journal of immunology (Baltimore, Md. : 1950)

Borken F,Markwart R,Requardt RP,Schubert K,Spacek M,Verner M,Rückriem S,Scherag A,Oehmichen F,Brunkhorst FM,Rubio I

Thu Jun 15 00:00:00 EDT 2017

Increased levels of CCR7(lo)PD-1(hi) CXCR5⁺ CD4⁺ T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure.

Experimental and therapeutic medicine

Gong Q,Zhu Y,Pang N,Ai H,Gong X,La X,Ding J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

Structure-guided development of a high-affinity human Programmed Cell Death-1: Implications for tumor immunotherapy.

EBioMedicine

Lázár-Molnár E,Scandiuzzi L,Basu I,Quinn T,Sylvestre E,Palmieri E,Ramagopal UA,Nathenson SG,Guha C,Almo SC

11-9969 was used in Flow cytometry/Cell sorting to highlight the value of structural considerations in the design of a PD-1 Ig fusion protein with immune modulatory properties.

Wed Mar 01 00:00:00 EST 2017

Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.

PloS one

Geißler K,Markwart R,Requardt RP,Weigel C,Schubert K,Scherag A,Rubio I,Guntinas-Lichius O

11-9969 was used in Flow cytometry/Cell sorting to analyse and characterise the response of tonsillar T-cells from chronic tonsillitis to antigenic stimulation.

Mon Oct 16 00:00:00 EDT 2017

Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype.

Journal of virology

de Alwis R,Bangs DJ,Angelo MA,Cerpas C,Fernando A,Sidney J,Peters B,Gresh L,Balmaseda A,de Silva AD,Harris E,Sette A,Weiskopf D

11-9969 was used in Flow cytometry/Cell sorting to define DENV-specific CD8(+)T cell responses in general and those of HLA alleles associated with dominant responses.

Sun May 01 00:00:00 EDT 2016

Tumor-infiltrating Tim-3⁺ T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk.

Oncoimmunology

Li J,Shayan G,Avery L,Jie HB,Gildener-Leapman N,Schmitt N,Lu BF,Kane LP,Ferris RL

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Oncoimmunology

Braun M,Ress ML,Yoo YE,Scholz CJ,Eyrich M,Schlegel PG,Wölfl M

11-9969 was used in Flow cytometry/Cell sorting to investigate how IL12, and other inflammatory cytokines, shape effector functions of human T-cells.

Fri Jul 01 00:00:00 EDT 2016

Monoclonal antibodies specific to human Δ42PD1: A novel immunoregulator potentially involved in HIV-1 and tumor pathogenesis.

mAbs

Cheng L,Tang X,Liu L,Peng J,Nishiura K,Cheung AK,Guo J,Wu X,Tang HY,An M,Zhou J,Cheung KW,Wang H,Guan X,Wu Z,Chen Z

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Tue Mar 22 00:00:00 EDT 2016

PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients.

British journal of cancer

Wu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S

11-9969 was used in Flow cytometry/Cell sorting to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients.

Tue Sep 23 00:00:00 EDT 2014

IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.

Journal of leukocyte biology

Jurado JO,Pasquinelli V,Alvarez IB,Peña D,Rovetta AI,Tateosian NL,Romeo HE,Musella RM,Palmero D,Chuluyán HE,García VE

11-9969 was used in Flow cytometry/Cell sorting to investigate the induction and regulation of IFN-γ and IL-17 during active human Mycobacterium tuberculosis infection.

Fri Jun 01 00:00:00 EDT 2012

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

The PD-1/PD-L1 axis modulates the natural killer cell versus multiple myeloma effect: a therapeutic target for CT-011, a novel monoclonal anti-PD-1 antibody.

Blood

Benson DM,Bakan CE,Mishra A,Hofmeister CC,Efebera Y,Becknell B,Baiocchi RA,Zhang J,Yu J,Smith MK,Greenfield CN,Porcu P,Devine SM,Rotem-Yehudar R,Lozanski G,Byrd JC,Caligiuri MA

11-9969 was used in Flow cytometry/Cell sorting to examine the NK cell response against MM, mediated by the PD-1/PD-L1 signalling axis and CT-011.

Thu Sep 30 00:00:00 EDT 2010

PD-1/PD-L1 expression in human T-cell leukemia virus type 1 carriers and adult T-cell leukemia/lymphoma patients.

Leukemia

Kozako T,Yoshimitsu M,Fujiwara H,Masamoto I,Horai S,White Y,Akimoto M,Suzuki S,Matsushita K,Uozumi K,Tei C,Arima N

11-9969 was used in Flow cytometry/Cell sorting to determine whether the PD-1/PD-L1 pathway could be involved in the establishment of persistent HTLV-1 infections.

Sun Feb 01 00:00:00 EST 2009

Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients.

Journal of immunology (Baltimore, Md. : 1950)

Takahashi N,Matsumoto K,Saito H,Nanki T,Miyasaka N,Kobata T,Azuma M,Lee SK,Mizutani S,Morio T

11-9969 was used in Flow cytometry/Cell sorting to investigate the effect of ICOS deficiency on T cell immune responses, showing that it leads to impaired CD4 and CD8 effector function and decreased memory T cell populations.

Fri May 01 00:00:00 EDT 2009

Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of lupus.

Journal of immunology (Baltimore, Md. : 1950)

Zhang L,Bertucci AM,Ramsey-Goldman R,Burt RK,Datta SK

11-9969 was used in Flow cytometry/Cell sorting to show that after hemopoietic stem cell posttransplant patients certain Treg populations return to levels seen in healthy patient.

Sun Nov 15 00:00:00 EST 2009

Spontaneous recovery in acute human hepatitis C virus infection: functional T-cell thresholds and relative importance of CD4 help.

Journal of virology

Smyk-Pearson S,Tester IA,Klarquist J,Palmer BE,Pawlotsky JM,Golden-Mason L,Rosen HR

11-9969 was used in Flow cytometry/Cell sorting to examine total HCV-specific CD4(+) and CD8(+) T-cell responses in acute HCV infection and identify functional T-cell thresholds that predict recovery.

Fri Feb 01 00:00:00 EST 2008

Programmed death-1 expression is associated with the disease status in hepatitis B virus infection.

World journal of gastroenterology

Ye P,Weng ZH,Zhang SL,Zhang JA,Zhao L,Dong JH,Jie SH,Pang R,Wei RH

11-9969 was used in Flow cytometry/Cell sorting to confirm a negative correlation between the level of PD-1 and the intensity of virus specific CD8+ T cell response.

Mon Jul 28 00:00:00 EDT 2008

FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of high-risk breast cancer patients: Implication for immunotherapy.

BMC cancer

Ghebeh H,Barhoush E,Tulbah A,Elkum N,Al-Tweigeri T,Dermime S

Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9969-42) in Immunohistochemistry

Sat Feb 23 00:00:00 EST 2008

PD-1 up-regulation is correlated with HIV-specific memory CD8+ T-cell exhaustion in typical progressors but not in long-term nonprogressors.

Blood

Zhang JY,Zhang Z,Wang X,Fu JL,Yao J,Jiao Y,Chen L,Zhang H,Wei J,Jin L,Shi M,Gao GF,Wu H,Wang FS

Fri Jun 01 00:00:00 EDT 2007

T regulatory cell levels decrease in people infected with Schistosoma mansoni on effective treatment.

The American journal of tropical medicine and hygiene

Watanabe K,Mwinzi PN,Black CL,Muok EM,Karanja DM,Secor WE,Colley DG

11-9969 was used in Flow cytometry/Cell sorting to investigate the role of Tregs in Schistosoma mansoni infection, showing that Treg levels decrease in people on effective treatment.

Mon Oct 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD279; hPD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

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Gene Aliases: CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; SLEB2

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UniProt ID: (Human) Q15116

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Entrez Gene ID: (Human) 5133

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Function(s)

signal transducer activity protein binding

Process(es)

negative regulation of tolerance induction apoptotic process humoral immune response signal transduction multicellular organism development T cell costimulation negative regulation of apoptotic process positive regulation of T cell apoptotic process

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CD279 (PD-1) Monoclonal Antibody (MIH4), FITC, eBioscience™

CD279 (PD-1) Antibody (11-9969-42) in Flow

Product Details

11-9969-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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