Invitrogen

CD279 (PD-1) Monoclonal Antibody (MIH4), eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

21 Published Figures

24 References

View all (275) PD-1 (CD279) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD279 (PD-1) Antibody in Western Blot (WB)

FIGURE: 1 / 25

CD279 (PD-1) Antibody (14-9969-82) in WB

Western blot was performed using CD279 (PD-1) Monoclonal Antibody (MIH4), eBioscience™; (Product # 14-9969-82) and a ~ 55 kDa band corresponding to CD279 (PD-1) was observed in mouse thymus and mouse tonsil which are reported to be positive and not in mouse brain. Tissue extracts (30 µg lysate) of mouse thymus (Lane 1), mouse tonsil (Lane 2) and mouse brain (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # ... View More

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

CD279 (PD-1) Antibody in Immunohistochemistry (IHC)

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Product Details

14-9969-82

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 20 publications 20 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MIH4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468643

Product Specific Information

Description: The MIH4 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than antibody clones J105.

Applications Reported: This MIH4 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested: This MIH4 antibody has been tested by flow cytometric analysis of PHA-stimulated peripheral blood mononuclear cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reprogramming of Tumor-reactive Tumor-infiltrating Lymphocytes to Human-induced Pluripotent Stem Cells.

Cancer research communications

Islam SMR,Maeda T,Tamaoki N,Good ML,Kishton RJ,Paria BC,Yu Z,Bosch-Marce M,Bedanova NM,Liu C,Kruhlak MJ,Restifo NP,Vizcardo R

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.

Signal transduction and targeted therapy

Zheng G,Guo Z,Li W,Xi W,Zuo B,Zhang R,Wen W,Yang AG,Jia L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Construction of Novel lncRNA-miRNA-mRNA Network Associated With Recurrence and Identification of Immune-Related Potential Regulatory Axis in Hepatocellular Carcinoma.

Frontiers in oncology

Zhan T,Gao X,Wang G,Li F,Shen J,Lu C,Xu L,Li Y,Zhang J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Imaging Changes and Immune-Checkpoint Expression on T Cells in Bronchoalveolar Lavage Fluid from Patients with Pulmonary Sarcoidosis.

Biomedicines

Kotetsu Y,Yanagihara T,Suzuki K,Ando H,Eto D,Hata K,Arimura-Omori M,Yamamoto Y,Harada E,Hamada N

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Thu Sep 16 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Immunogenic neoantigens derived from gene fusions stimulate T cell responses.

Nature medicine

Yang W,Lee KW,Srivastava RM,Kuo F,Krishna C,Chowell D,Makarov V,Hoen D,Dalin MG,Wexler L,Ghossein R,Katabi N,Nadeem Z,Cohen MA,Tian SK,Robine N,Arora K,Geiger H,Agius P,Bouvier N,Huberman K,Vanness K,Havel JJ,Sims JS,Samstein RM,Mandal R,Tepe J,Ganly I,Ho AL,Riaz N,Wong RJ,Shukla N,Chan TA,Morris LGT

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Immune Heterogeneity Between Primary Tumors and Corresponding Metastatic Lesions and Response to Platinum Therapy in Primary Ovarian Cancer.

Cancers

Dötzer K,Schlüter F,Schoenberg MB,Bazhin AV,von Koch FE,Schnelzer A,Anthuber S,Grab D,Czogalla B,Burges A,Werner J,Mahner S,Mayer B

14-9969 was used in Immunohistochemistry to show that immune heterogeneity was associated with platinum response and might represent a selection marker for personalized therapy.

Mon Aug 26 00:00:00 EDT 2019

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.

The Journal of experimental medicine

Seki A,Rutz S

14-9969 was used in Flow cytometry/Cell sorting to develop an optimised approach for Cas9/RNP transfection of primary mouse and human T cells without TCR stimulation, that results in near complete loss of target gene expression at the population level.

Mon Mar 05 00:00:00 EST 2018

Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells.

Cell reports

Zhao Y,Harrison DL,Song Y,Ji J,Huang J,Hui E

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Tue Jul 10 00:00:00 EDT 2018

Increased levels of CCR7(lo)PD-1(hi) CXCR5⁺ CD4⁺ T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure.

Experimental and therapeutic medicine

Gong Q,Zhu Y,Pang N,Ai H,Gong X,La X,Ding J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

Tumor-infiltrating Tim-3⁺ T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk.

Oncoimmunology

Li J,Shayan G,Avery L,Jie HB,Gildener-Leapman N,Schmitt N,Lu BF,Kane LP,Ferris RL

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.

Oncotarget

Komdeur FL,Wouters MC,Workel HH,Tijans AM,Terwindt AL,Brunekreeft KL,Plat A,Klip HG,Eggink FA,Leffers N,Helfrich W,Samplonius DF,Bremer E,Wisman GB,Daemen T,Duiker EW,Hollema H,Nijman HW,de Bruyn M

14996982 was used in flow cytometry to define the contribution of CD103+ tumor-infiltrating lymphocytes to high-grade serous epithelial ovarian cancer

Tue Nov 15 00:00:00 EST 2016

Monoclonal antibodies specific to human Δ42PD1: A novel immunoregulator potentially involved in HIV-1 and tumor pathogenesis.

mAbs

Cheng L,Tang X,Liu L,Peng J,Nishiura K,Cheung AK,Guo J,Wu X,Tang HY,An M,Zhou J,Cheung KW,Wang H,Guan X,Wu Z,Chen Z

14-9969 was used in Flow cytometry/Cell sorting to generate high-affinity mAbs from δ42PD1-immunised mice, that can be used to further investigate the role of δ42PD1 in tumour pathogenesis.

Tue Mar 22 00:00:00 EDT 2016

Monoclonal antibodies specific to human Δ42PD1: A novel immunoregulator potentially involved in HIV-1 and tumor pathogenesis.

mAbs

Cheng L,Tang X,Liu L,Peng J,Nishiura K,Cheung AK,Guo J,Wu X,Tang HY,An M,Zhou J,Cheung KW,Wang H,Guan X,Wu Z,Chen Z

14-9969 was used in Flow cytometry/Cell sorting to generate high-affinity mAbs from δ42PD1-immunised mice, that can be used to further investigate the role of δ42PD1 in tumour pathogenesis.

Tue Mar 22 00:00:00 EDT 2016

Antigen-dependent integration of opposing proximal TCR-signaling cascades determines the functional fate of T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Wolchinsky R,Hod-Marco M,Oved K,Shen-Orr SS,Bendall SC,Nolan GP,Reiter Y

14-9969 was used in Flow cytometry/Cell sorting to investigate how T cells measure antigen dose and how this determines functional output.

Sat Mar 01 00:00:00 EST 2014

Efficient Nef-mediated downmodulation of TCR-CD3 and CD28 is associated with high CD4+ T cell counts in viremic HIV-2 infection.

Journal of virology

Khalid M,Yu H,Sauter D,Usmani SM,Schmokel J,Feldman J,Gruters RA,van der Ende ME,Geyer M,Rowland-Jones S,Osterhaus AD,Kirchhoff F

14-9969 was used in Flow cytometry/Cell sorting to elucidate the role of the Nef protein in enhancing functionality of immune cells and in the immunopathogenesis of HIV-2 infection.

Tue May 01 00:00:00 EDT 2012

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

The PD-1/PD-L1 axis modulates the natural killer cell versus multiple myeloma effect: a therapeutic target for CT-011, a novel monoclonal anti-PD-1 antibody.

Blood

Benson DM,Bakan CE,Mishra A,Hofmeister CC,Efebera Y,Becknell B,Baiocchi RA,Zhang J,Yu J,Smith MK,Greenfield CN,Porcu P,Devine SM,Rotem-Yehudar R,Lozanski G,Byrd JC,Caligiuri MA

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Thu Sep 30 00:00:00 EDT 2010

Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

Journal of immunology (Baltimore, Md. : 1950)

Trabattoni D,Saresella M,Pacei M,Marventano I,Mendozzi L,Rovaris M,Caputo D,Borelli M,Clerici M

14-9969 was used in Blocking experiments to indicate the important role of the PD-1/PD-L1 costimulatory pathway in modulating disease activity in multiple sclerosis.

Thu Oct 15 00:00:00 EDT 2009

Neuron-interacting satellite glial cells in human trigeminal ganglia have an APC phenotype.

Journal of immunology (Baltimore, Md. : 1950)

van Velzen M,Laman JD,Kleinjan A,Poot A,Osterhaus AD,Verjans GM

14-9969 was used in Immunohistochemistry on frozen tissues to indicate a role for TG-glial cells as resident APCs with potential T cell modulatory properties.

Sat Aug 15 00:00:00 EDT 2009

FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of high-risk breast cancer patients: Implication for immunotherapy.

BMC cancer

Ghebeh H,Barhoush E,Tulbah A,Elkum N,Al-Tweigeri T,Dermime S

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Immunohistochemistry

Sat Feb 23 00:00:00 EST 2008

PD-1 up-regulation is correlated with HIV-specific memory CD8+ T-cell exhaustion in typical progressors but not in long-term nonprogressors.

Blood

Zhang JY,Zhang Z,Wang X,Fu JL,Yao J,Jiao Y,Chen L,Zhang H,Wei J,Jin L,Shi M,Gao GF,Wu H,Wang FS

Fri Jun 01 00:00:00 EDT 2007

Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation.

The Journal of experimental medicine

Freeman GJ,Long AJ,Iwai Y,Bourque K,Chernova T,Nishimura H,Fitz LJ,Malenkovich N,Okazaki T,Byrne MC,Horton HF,Fouser L,Carter L,Ling V,Bowman MR,Carreno BM,Collins M,Wood CR,Honjo T

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9969-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

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Gene Aliases: CD279; hPD-1; hPD-l; hSLE1; Ly101; PD-1; PD1; Pdc1; PDCD1; SLEB2

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UniProt ID: (Human) Q15116, (Mouse) Q02242

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Entrez Gene ID: (Human) 5133, (Mouse) 18566

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Function(s)

signal transducer activity protein binding

Process(es)

negative regulation of tolerance induction apoptotic process humoral immune response signal transduction multicellular organism development T cell costimulation negative regulation of apoptotic process positive regulation of T cell apoptotic process immune system process positive regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD279 (PD-1) Monoclonal Antibody (MIH4), eBioscience™

CD279 (PD-1) Antibody (14-9969-82) in WB

Product Details

14-9969-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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