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Invitrogen
CD28 Recombinant Human Monoclonal Antibody (CD28.2)
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
CD28 Antibody (740009H) in Flow
Jurkat cells were stained with 0.25 µg of CD28 Recombinant Human Monoclonal Antibody (CD28.2) (Product # 740009H), followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Product # A11013, 1:500) (pink histogram) or left unstained (blue histogram). Viable cells were used for analysis, as determined by LIVE/DEAD™ Fixable Viability Dye eFluor™ 780 (Product # 65-0865-14). The flow cytometry data was acquired using Attune™ NxT Flow Cytometer (Product # A29004).
Product Details
740009H
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Human
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
CD28.2
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Research Use Only (RUO) human recombinant monoclonal antibodies are produced using in vitro expression systems and ideal to use as controls for development of assays from human samples. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
This product is a humanized format of a mouse monoclonal antibody.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44
Gene Aliases: CD28; Tp44
UniProt ID: (Human) P10747
Entrez Gene ID: (Human) 940
positive regulation of inflammatory response to antigenic stimulus
humoral immune response
cell surface receptor signaling pathway
positive regulation of gene expression
negative regulation of gene expression
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-4 production
cytokine biosynthetic process
positive regulation of T cell proliferation
negative thymic T cell selection
regulatory T cell differentiation
positive regulation of viral genome replication
positive regulation of interleukin-2 biosynthetic process
regulation of regulatory T cell differentiation
positive regulation of translation
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell proliferation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of isotype switching to IgG isotypes
regulation of defense response to virus by virus
T cell receptor signaling pathway
positive regulation of protein kinase B signaling
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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