Invitrogen

CD282 (TLR2) Monoclonal Antibody (TL2.1), FITC, eBioscience™

CD282 (TLR2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CD282 (TLR2) Antibody (11-9922-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (open histogram) or Anti-Human CD282 (TLR2) FITC (filled histogram). Cells in the monocyte gate were used for analysis.

CD282 (TLR2) Antibody in Immunocytochemistry (ICC/IF)

CD282 (TLR2) Antibody in Western Blot (WB)

Product Image Gallery

Product Details

11-9922-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 27 publications 27 publications

Functional Assay (Functional)

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TL2.1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042717

Product Specific Information

Description: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported: The TL2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TL2.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Peptidoglycan mediates Leptospira outer membrane protein Loa22 to toll-like receptor 2 for inflammatory interaction: a novel innate immune recognition.

Scientific reports

Hsu SH,Chang MY,Lin SM,Ko YC,Chou LF,Tian YC,Hung CC,Yang CW

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Immunocytochemistry

Wed Jan 13 00:00:00 EST 2021

Peptidoglycan mediates Leptospira outer membrane protein Loa22 to toll-like receptor 2 for inflammatory interaction: a novel innate immune recognition.

Scientific reports

Hsu SH,Chang MY,Lin SM,Ko YC,Chou LF,Tian YC,Hung CC,Yang CW

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Immunocytochemistry

Wed Jan 13 00:00:00 EST 2021

Blockade of TLR2 and TLR4 Attenuates Inflammatory Response and Parasite Load in Cutaneous Leishmaniasis.

Frontiers in immunology

Carneiro PP,Dórea AS,Oliveira WN,Guimarães LH,Brodskyn C,Carvalho EM,Bacellar O

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Mon Dec 13 00:00:00 EST 2021

TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis.

Nature communications

Aguilar-Briseño JA,Upasani V,Ellen BMT,Moser J,Pauzuolis M,Ruiz-Silva M,Heng S,Laurent D,Choeung R,Dussart P,Cantaert T,Smit JM,Rodenhuis-Zybert IA

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Tue Jun 23 00:00:00 EDT 2020

Activation of the Toll‑like receptor 2 signaling pathway inhibits the proliferation of HCC cells in vitro.

Oncology reports

Chen Y,Huang Z,Chen X,Ye H

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production.

Frontiers in immunology

Faraj TA,Stover C,Erridge C

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Mon Oct 19 00:00:00 EDT 2020

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria.

PloS one

Graustein AD,Misch EA,Musvosvi M,Shey M,Shah JA,Seshadri C,Ajuogu A,Bowman K,Mulenga H,Veldsman A,Hanekom WA,Hatherill M,Scriba TJ,Hawn TR

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9922-42) in Flow Cytometry

Tue May 07 00:00:00 EDT 2019

DHRS9 Is a Stable Marker of Human Regulatory Macrophages.

Transplantation

Riquelme P,Amodio G,Macedo C,Moreau A,Obermajer N,Brochhausen C,Ahrens N,Kekarainen T,Fändrich F,Cuturi C,Gregori S,Metes D,Schlitt HJ,Thomson AW,Geissler EK,Hutchinson JA

Wed Nov 01 00:00:00 EDT 2017

Innate inflammatory gene expression profiling in potential brain-dead donors: detailed investigation of the effect of common corticosteroid therapy.

Innate immunity

Gholamnezhadjafari R,Tajik N,Falak R,Aflatoonian R,Dehghan S,Rezaei A

Sat Jul 01 00:00:00 EDT 2017

Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.

European journal of immunology

Cheng WC,van Asten SD,Burns LA,Evans HG,Walter GJ,Hashim A,Hughes FJ,Taams LS

11-9922 was used in Flow cytometry/Cell sorting to investigate the mechanism by which the periodontal pathogens promote a Th17/IL-17 response in vitro, showing that they activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.

Thu Sep 01 00:00:00 EDT 2016

Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma.

PloS one

Mastorci K,Muraro E,Pasini E,Furlan C,Sigalotti L,Cinco M,Dolcetti R,Fratta E

11-9922 was used in Flow cytometry/Cell sorting to investigate the contribution of Toll-like receptor 1/2 and 5 to the development of mantle cell lymphoma.

Tue Feb 21 00:00:00 EST 2017

Innate Immune Responses in the Neutrophils of Community Dwelling and Nursing Home Elders.

Journal of aging science

Juthani-Mehta M,Guo X,Shaw AC,Towle V,Ning Y,Wang X,Allore HG,Fikrig E,Montgomery RR

11-9922 was used in Flow cytometry/Cell sorting to evaluate innate immune responses of older disabled nursing home residents that may contribute to infectious disease susceptibility.

Mon Jan 27 00:00:00 EST 2014

Celecoxib sensitizes Staphylococcus aureus to antibiotics in macrophages by modulating SIRT1.

PloS one

Annamanedi M,Kalle AM

11-9922 was used in Flow cytometry/Cell sorting to demonstrate a novel role of celecoxib in controlling inflammation as an enhancer of antibiotic activity against bacteria by modulating SIRT1.

Tue Oct 13 00:00:00 EDT 2015

Porphyromonas Gingivalis and E-coli induce different cytokine production patterns in pregnant women.

PloS one

Faas MM,Kunnen A,Dekker DC,Harmsen HJ,Aarnoudse JG,Abbas F,De Vos P,Van Pampus MG

11-9922 was used in Flow cytometry/Cell sorting to demonstrate that the different responses of pregnant women to Porphyromonas Gingivalis and E-coli may result from variations in cytokine production.

Tue Nov 04 00:00:00 EST 2014

IL-29 enhances Toll-like receptor-mediated IL-6 and IL-8 production by the synovial fibroblasts from rheumatoid arthritis patients.

Arthritis research & therapy

Xu L,Feng X,Tan W,Gu W,Guo D,Zhang M,Wang F

11-9922 was used in Flow cytometry/Cell sorting to investigate the effect of IL-29 on cytokine production in synovial fibroblasts in arthritis patients.

Tue Oct 29 00:00:00 EDT 2013

Identification of lineage relationships and novel markers of blood and skin human dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Harman AN,Bye CR,Nasr N,Sandgren KJ,Kim M,Mercier SK,Botting RA,Lewin SR,Cunningham AL,Cameron PU

11-9922 was used in Flow cytometry/Cell sorting to study the lineage relationships between blood and tissue dendritic cells, providing novel markers of blood and skin human dendritic cells.

Tue Jan 01 00:00:00 EST 2013

Vitamin D deficiency impacts on expression of toll-like receptor-2 and cytokine profile: a pilot study.

Journal of translational medicine

Ojaimi S,Skinner NA,Strauss BJ,Sundararajan V,Woolley I,Visvanathan K

11-9922 was used in Flow cytometry/Cell sorting to add evidence to the important role of vitamin D in innate immunity, possibly helping to reduce inflammation.

Mon Jul 22 00:00:00 EDT 2013

Monocyte subpopulations from pre-eclamptic patients are abnormally skewed and exhibit exaggerated responses to Toll-like receptor ligands.

PloS one

Al-ofi E,Coffelt SB,Anumba DO

11-9922 was used in Flow cytometry/Cell sorting to analyse monocytic subpopulations in pre-eclampsia patients and normal pregnant controls.

Thu Jan 10 00:00:00 EST 2013

A single nucleotide polymorphism in 3'-untranslated region contributes to the regulation of Toll-like receptor 4 translation.

The Journal of biological chemistry

Sato K,Yoshimura A,Kaneko T,Ukai T,Ozaki Y,Nakamura H,Li X,Matsumura H,Hara Y,Ogata Y

11-9922 was used in Flow cytometry/Cell sorting to indicate that genetic variation of rs11536889 contributes to translational regulation of TLR4, possibly by binding to microRNAs.

Fri Jul 20 00:00:00 EDT 2012

1,25-dihydroxyvitamin D3 modulates cytokine production induced by Candida albicans: impact of seasonal variation of immune responses.

The Journal of infectious diseases

Khoo AL,Chai LY,Koenen HJ,Kullberg BJ,Joosten I,van der Ven AJ,Netea MG

11-9922 was used in Flow cytometry/Cell sorting to show how vitamin D(3) skews Candida albicans induced cytokine responses towards an anti-inflammatory profile due to suppressed TLR2/4, Dectin-1 and MR transcription.

Sat Jan 01 00:00:00 EST 2011

Differential monocyte responses to TLR ligands in children with autism spectrum disorders.

Brain, behavior, and immunity

Enstrom AM,Onore CE,Van de Water JA,Ashwood P

11-9922 was used in Flow cytometry/Cell sorting to demonstrate that monocyte cultures from children with autism spectrum disorders are more responsive to signaling via select TLRs.

Fri Jan 01 00:00:00 EST 2010

MHC class II molecules enhance Toll-like receptor mediated innate immune responses.

PloS one

Frei R,Steinle J,Birchler T,Loeliger S,Roduit C,Steinhoff D,Seibl R,Büchner K,Seger R,Reith W,Lauener RP

11-9922-42 was used in Immunocytochemistry-immunoflourescence to demonstrate that major histocompatibility complex class II molecules are implicated in generating optimal toll-like receptor mediated innate immune responses.

Wed Jan 20 00:00:00 EST 2010

Targeting of DC-SIGN on human dendritic cells by minor fimbriated Porphyromonas gingivalis strains elicits a distinct effector T cell response.

Journal of immunology (Baltimore, Md. : 1950)

Zeituni AE,Jotwani R,Carrion J,Cutler CW

11-9922 was used in Flow cytometry/Cell sorting to investigate the DC receptors involved in the uptake of P. gingivalis and the induced effector immune response.

Sun Nov 01 00:00:00 EDT 2009

The Journal of investigative dermatology

Mandron M,Ariès MF,Boralevi F,Martin H,Charveron M,Taieb A,Davrinche C

Tue Apr 01 00:00:00 EDT 2008

Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease.

Rheumatology (Oxford, England)

Do JE,Kwon SY,Park S,Lee ES

Sun Jun 01 00:00:00 EDT 2008

Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.

Veterinary immunology and immunopathology

Burgener IA,Jungi TW

Tue Jul 15 00:00:00 EDT 2008

Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC).

Veterinary immunology and immunopathology

Bonnefont-Rebeix C,Marchal T,Bernaud J,Pin JJ,Leroux C,Lebecque S,Chabanne L,Rigal D

Sun Jul 15 00:00:00 EDT 2007

Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner.

The Journal of pathology

Chan G,Guilbert LJ

Fri Sep 01 00:00:00 EDT 2006

Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner.

The Journal of pathology

Chan G,Guilbert LJ

Fri Sep 01 00:00:00 EDT 2006

Expansion of toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process.

Arthritis and rheumatism

Papadimitraki ED,Choulaki C,Koutala E,Bertsias G,Tsatsanis C,Gergianaki I,Raptopoulou A,Kritikos HD,Mamalaki C,Sidiropoulos P,Boumpas DT

11-9922 was used in Flow cytometry/Cell sorting to examine the expression and function of various TLRs in subpopulations of peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

Wed Nov 01 00:00:00 EST 2006

In tuberculous pleural effusions, activated neutrophils undergo apoptosis and acquire a dendritic cell-like phenotype.

The Journal of infectious diseases

Aleman M,de la Barrera SS,Schierloh PL,Alves L,Yokobori N,Baldini M,Abbate E,Sasiain MC

11-9922 was used in Flow cytometry/Cell sorting to demonstrate that, in tuberculous pleuritis, neutrophil apoptosis increases together with the expression of Toll-like receptor 2 and phosphorylated p38 kinase.

Mon Aug 01 00:00:00 EDT 2005

Toll-like receptor 2 expression on human conjunctival epithelial cells: a pathway for Staphylococcus aureus involvement in chronic ocular proinflammatory responses.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology

Cook EB,Stahl JL,Esnault S,Barney NP,Graziano FM

Fri Apr 01 00:00:00 EST 2005

Regulation of Toll-like receptor (TLR)2 and TLR4 on CD14dimCD16+ monocytes in response to sepsis-related antigens.

Clinical and experimental immunology

Skinner NA,MacIsaac CM,Hamilton JA,Visvanathan K

11-9922 was used in Flow cytometry/Cell sorting to hypothesize that following exposure to bacterial antigens, subsequent TNF-alpha drives a differentiation of monocytes into a CD14(dim)CD16(+) subpopulation.

Mon Aug 01 00:00:00 EDT 2005

Effect of deletion or overexpression of the 19-kilodalton lipoprotein Rv3763 on the innate response to Mycobacterium tuberculosis.

Infection and immunity

Stewart GR,Wilkinson KA,Newton SM,Sullivan SM,Neyrolles O,Wain JR,Patel J,Pool KL,Young DB,Wilkinson RJ

11-9922 was used in Flow cytometry/Cell sorting to study the interplay between phagocytes and the Mycobacterium tuberculosis 19-kDa lipoprotein during infection.

Sat Oct 01 00:00:00 EDT 2005

Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium.

Journal of immunology (Baltimore, Md. : 1950)

Ueta M,Nochi T,Jang MH,Park EJ,Igarashi O,Hino A,Kawasaki S,Shikina T,Hiroi T,Kinoshita S,Kiyono H

Wed Sep 01 00:00:00 EDT 2004

Mycobacterium tuberculosis triggers apoptosis in peripheral neutrophils involving toll-like receptor 2 and p38 mitogen protein kinase in tuberculosis patients.

Infection and immunity

Alemán M,Schierloh P,de la Barrera SS,Musella RM,Saab MA,Baldini M,Abbate E,Sasiain MC

11-9922 was used in Flow cytometry/Cell sorting to determine the mechanisms involved in spontaneous and Mycobacterium tuberculosis-induced apoptosis in peripheral neutrophils.

Wed Sep 01 00:00:00 EDT 2004

Expression of Toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis.

Arthritis and rheumatism

Iwahashi M,Yamamura M,Aita T,Okamoto A,Ueno A,Ogawa N,Akashi S,Miyake K,Godowski PJ,Makino H

Sat May 01 00:00:00 EDT 2004

The urokinase receptor can be induced by Borrelia burgdorferi through receptors of the innate immune system.

Infection and immunity

Coleman JL,Benach JL

Wed Oct 01 00:00:00 EDT 2003

Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung.

Histochemistry and cell biology

Droemann D,Goldmann T,Branscheid D,Clark R,Dalhoff K,Zabel P,Vollmer E

Sat Feb 01 00:00:00 EST 2003

Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis.

Oral microbiology and immunology

Mori Y,Yoshimura A,Ukai T,Lien E,Espevik T,Hara Y

Sat Feb 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

View more View less

Gene Aliases: CD282; TIL4; TLR2

View more View less

UniProt ID: (Human) O60603

View more View less

Entrez Gene ID: (Human) 7097

View more View less

Function(s)

lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD282 (TLR2) Monoclonal Antibody (TL2.1), FITC, eBioscience™

CD282 (TLR2) Antibody (11-9922-42) in Flow

Product Details

11-9922-42

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!