Invitrogen

CD283 (TLR3) Monoclonal Antibody (TLR3.7), Biotin, eBioscience™

4 Published Figures
7 References
View all (26) TLR3 (CD283) antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

13-9039-80

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test
View 4 publications 4 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TLR3.7

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466967

Product Specific Information

Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface.

Applications Reported: TLR3.7 has been reported for use in flow cytometric analysis. For optimal detection of TLR3 with this mAb by flow cytometry, PE-conjugated or Biotin-conjugated TLR3.7 (revealed with SAV PE, Product # 12-4312) are recommended.

Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the cell surface. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD283; TLR 3; Toll-like receptor 3

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Gene Aliases: CD283; IIAE2; TLR3

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UniProt ID: (Human) O15455

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Entrez Gene ID: (Human) 7098

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Function(s)
double-stranded RNA binding receptor activity transmembrane signaling receptor activity protein binding identical protein binding
Process(es)
toll-like receptor signaling pathway microglial cell activation involved in immune response regulation of dendritic cell cytokine production MyD88-independent toll-like receptor signaling pathway inflammatory response hyperosmotic response signal transduction I-kappaB kinase/NF-kappaB signaling activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation male gonad development detection of virus positive regulation of gene expression positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of toll-like receptor signaling pathway negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 3 signaling pathway positive regulation of type III interferon production cellular response to interferon-beta TRIF-dependent toll-like receptor signaling pathway cellular response to drug positive regulation of NF-kappaB import into nucleus defense response to bacterium positive regulation of apoptotic process response to exogenous dsRNA positive regulation of interferon-gamma biosynthetic process positive regulation of chemokine biosynthetic process innate immune response positive regulation of interferon-alpha biosynthetic process positive regulation of interferon-beta biosynthetic process negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of inflammatory response positive regulation of NF-kappaB transcription factor activity defense response to virus necroptotic process cellular response to mechanical stimulus cellular response to interferon-gamma cellular response to exogenous dsRNA apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway
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