Invitrogen

CD284 (TLR4) Monoclonal Antibody (HTA125), APC, eBioscience™

CD284 (TLR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 15

CD284 (TLR4) Antibody (17-9917-42) in Flow

Staining of normal human peripheral blood cells (gated on monocytes) (left) or human TLR4/MD-2-transfected cells (right) with Mouse IgG2a kappa Isotype Control APC (open histogram) or Anti-Human CD284 (TLR4) APC (filled histogram).

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Product Details

17-9917-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 24 publications 24 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HTA125

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016689

Product Specific Information

Description: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.

It is highly recommended that for optimal staining of TLR4, whole blood be stained using the lysed whole blood protocol (found in Best Protocols) rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.

Applications Reported: HTA125 has been reported for use in flow cytometric analysis.

Applications Tested: This HTA125 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.

Cell reports

Di Nardo A,Chang YL,Alimohammadi S,Masuda-Kuroki K,Wang Z,Sriram K,Insel PA

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Tue May 30 00:00:00 EDT 2023

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

E-cigarettes and health risks: more to the flavor than just the name.

American journal of physiology. Lung cellular and molecular physiology

Ween MP,Moshensky A,Thredgold L,Bastian NA,Hamon R,Badiei A,Nguyen PT,Herewane K,Jersmann H,Bojanowski CM,Shin J,Reynolds PN,Crotty Alexander LE,Hodge SJ

17-9917 was used in Flow cytometry/Cell sorting to investigate the flavouring profile of 10 different flavoured E-liquids. We assessed bronchial epithelial cell viability and apoptosis,.

Thu Apr 01 00:00:00 EDT 2021

Grindelia squarrosa Extract and Grindelic Acid Modulate Pro-inflammatory Functions of Respiratory Epithelium and Human Macrophages.

Frontiers in pharmacology

Gierlikowska B,Filipek A,Gierlikowski W,Kania D,Stefańska J,Demkow U,Kiss AK

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Sat Feb 06 00:00:00 EST 2021

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells.

International journal of molecular medicine

Suzuki K,Ohkuma M,Nagaoka I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function.

Scientific reports

Hamon R,Tran HB,Roscioli E,Ween M,Jersmann H,Hodge S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function.

Scientific reports

Hamon R,Tran HB,Roscioli E,Ween M,Jersmann H,Hodge S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma.

Oncotarget

Tobouti PL,Bolt R,Radhakrishnan R,de Sousa SCOM,Hunter KD

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

A low dose lipid infusion is sufficient to induce insulin resistance and a pro-inflammatory response in human subjects.

PloS one

Liang H,Lum H,Alvarez A,Garduno-Garcia JJ,Daniel BJ,Musi N

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2018

S100A9 induces differentiation of acute myeloid leukemia cells through TLR4.

Blood

Laouedj M,Tardif MR,Gil L,Raquil MA,Lachhab A,Pelletier M,Tessier PA,Barabé F

Thu Apr 06 00:00:00 EDT 2017

Phagocytosis and Inflammation: Exploring the effects of the components of E-cigarette vapor on macrophages.

Physiological reports

Ween MP,Whittall JJ,Hamon R,Reynolds PN,Hodge SJ

17-9917 was used in Flow cytometry/Cell sorting to conclude that E-cigarettes can cause macrophage phagocytic dysfunction, expression of phagocytic recognition receptors and cytokine secretion pathways.

Tue Aug 01 00:00:00 EDT 2017

Phagocytosis and Inflammation: Exploring the effects of the components of E-cigarette vapor on macrophages.

Physiological reports

Ween MP,Whittall JJ,Hamon R,Reynolds PN,Hodge SJ

Tue Aug 01 00:00:00 EDT 2017

Pro-inflammatory hepatic macrophages generate ROS through NADPH oxidase 2 via endocytosis of monomeric TLR4-MD2 complex.

Nature communications

Kim SY,Jeong JM,Kim SJ,Seo W,Kim MH,Choi WM,Yoo W,Lee JH,Shim YR,Yi HS,Lee YS,Eun HS,Lee BS,Chun K,Kang SJ,Kim SC,Gao B,Kunos G,Kim HM,Jeong WI

17-9917-42 was used in Flow Cytometry to show that palmitate-stimulated CD11b+F4/80low hepatic infiltrating macrophages generate ROS via dynamin-mediated endocytosis of TLR4 and NOX2, independently from MyD88 and TRIF.

Thu Dec 21 00:00:00 EST 2017

Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels.

PloS one

Steiner M,Hawranek T,Schneider M,Ferreira F,Horejs-Hoeck J,Harrer A,Himly M

17-9917 was used in Flow cytometry/Cell sorting to demonstrate that decreased TLR expression and elevated CXCL8 secretion can exacerbate allergic symptoms of parasitic infection.

Tue Jul 26 00:00:00 EDT 2016

Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.

Journal of immunology (Baltimore, Md. : 1950)

Giugliano S,Petroff MG,Warren BD,Jasti S,Linscheid C,Ward A,Kramer A,Dobrinskikh E,Sheiko MA,Gale M,Golden-Mason L,Winn VD,Rosen HR

17-9917 was used in Flow cytometry/Cell sorting to demonstrate that HCV-RNA sensing by human trophoblasts induces recruitment and activation of innate immune cells.

Thu Oct 15 00:00:00 EDT 2015

TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.

PloS one

Avlas O,Bragg A,Fuks A,Nicholson JD,Farkash A,Porat E,Aravot D,Levy-Drummer RS,Cohen C,Shainberg A,Arad M,Hochhauser E

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2016

HMGB1 induction of clusterin creates a chemoresistant niche in human prostate tumor cells.

Scientific reports

Zhou J,Chen X,Gilvary DL,Tejera MM,Eksioglu EA,Wei S,Djeu JY

17-9917 was used in Flow cytometry/Cell sorting to study seminal crosstalk between dying and residual live tumour cells during treatment with docetaxel that can result in outgrowth of a chemoresistant population.

Thu Oct 15 00:00:00 EDT 2015

Impaired neutrophil extracellular trap formation: a novel defect in the innate immune system of aged individuals.

Aging cell

Hazeldine J,Harris P,Chapple IL,Grant M,Greenwood H,Livesey A,Sapey E,Lord JM

17-9917 was used in Flow cytometry/Cell sorting to identify that impaired neutrophil extracellular trap formation is a novel defect of innate immunity in older adults.

Fri Aug 01 00:00:00 EDT 2014

The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane.

Science signaling

Liaunardy-Jopeace A,Bryant CE,Gay NJ

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Tue Jul 29 00:00:00 EDT 2014

Negative regulation of Toll-like receptor 4 signaling by IL-10-dependent microRNA-146b.

Proceedings of the National Academy of Sciences of the United States of America

Curtale G,Mirolo M,Renzi TA,Rossato M,Bazzoni F,Locati M

17-9917 was used in Flow cytometry/Cell sorting to identify miR-146b as an IL-10-responsive miR with an anti-inflammatory activity based on multiple targeting of components of the TLR4 pathway in monocytes.

Tue Jul 09 00:00:00 EDT 2013

Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling.

The Journal of biological chemistry

Bretz NP,Ridinger J,Rupp AK,Rimbach K,Keller S,Rupp C,Marmé F,Umansky L,Umansky V,Eigenbrod T,Sammar M,Altevogt P

17-9917 was used in Flow cytometry/Cell sorting to suggest that exosomes trigger TLR-dependent signaling pathways in monocytic precursor cells but possibly also in other immune cells.

Fri Dec 20 00:00:00 EST 2013

Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling.

The Journal of biological chemistry

Bretz NP,Ridinger J,Rupp AK,Rimbach K,Keller S,Rupp C,Marmé F,Umansky L,Umansky V,Eigenbrod T,Sammar M,Altevogt P

17-9917 was used in Flow cytometry/Cell sorting to suggest that exosomes trigger TLR-dependent signaling pathways in monocytic precursor cells but possibly also in other immune cells.

Fri Dec 20 00:00:00 EST 2013

Bone marrow mononuclear cells up-regulate toll-like receptor expression and produce inflammatory mediators in response to cigarette smoke extract.

PloS one

Zhou J,Eksioglu EA,Fortenbery NR,Chen X,Wang H,Epling-Burnette PK,Djeu JY,Wei S

17-9917 was used in Flow cytometry/Cell sorting to elucidate the effects of cigarette smoke on human bone marrow haematopoiesis and characterise the inflammatory response.

Thu Dec 01 00:00:00 EST 2011

Candesartan inhibits Toll-like receptor expression and activity both in vitro and in vivo.

Atherosclerosis

Dasu MR,Riosvelasco AC,Jialal I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 17-9917-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4.

Microbes and infection

de Graaf R,Kloppenburg G,Kitslaar PJ,Bruggeman CA,Stassen F

Thu Jun 01 00:00:00 EDT 2006

Expression and involvement of Toll-like receptors (TLR)2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis.

Medical science monitor : international medical journal of experimental and clinical research

Mirlashari MR,Lyberg T

Fri Aug 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CD284; homolog of Drosophila toll; hToll; Toll-like receptor 4

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Gene Aliases: ARMD10; CD284; TLR-4; TLR4; TOLL

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UniProt ID: (Human) O00206

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Entrez Gene ID: (Human) 7099

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Function(s)

lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD284 (TLR4) Monoclonal Antibody (HTA125), APC, eBioscience™

CD284 (TLR4) Antibody (17-9917-42) in Flow

Product Details

17-9917-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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