Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), FITC, eBioscience™

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

19 Published Figures

31 References

View all (125) Integrin beta 1 (CD29) antibodies

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Protocols

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CD29 (Integrin beta 1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

CD29 (Integrin beta 1) Antibody (11-0299-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD29 FITC (purple histogram). Cells in the monocyte (left) or lymphocyte (right) gates were used for analysis.

CD29 (Integrin beta 1) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

11-0299-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Human

Published species

Cat, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TS2/16

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2043829

Product Specific Information

Description: The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta 1 integrins.

Applications Reported: This TS2/16 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TS2/16 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human osteoarthritic articular cartilage stem cells suppress osteoclasts and improve subchondral bone remodeling in experimental knee osteoarthritis partially by releasing TNFAIP3.

Stem cell research & therapy

Li ZL,Li XT,Hao RC,Wang FY,Wang YX,Zhao ZD,Li PL,Yin BF,Mao N,Ding L,Zhu H

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Wed Sep 27 00:00:00 EDT 2023

Comparative proteomic analysis of the mitochondria of menstrual stem cells and ovarian cancer cells.

Experimental and therapeutic medicine

Chen X,Zhong W,Chang Y,Song T,Liu B,Kong X,Kong Q

11-0299-42 was used in Flow cytometry/Cell sorting to investigate the potential effects of MenSC-derived mitochondria on ovarian cancer from the perspective of protein expression profiling.

Wed Feb 01 00:00:00 EST 2023

Comparative proteomic analysis of the mitochondria of menstrual stem cells and ovarian cancer cells.

Experimental and therapeutic medicine

Chen X,Zhong W,Chang Y,Song T,Liu B,Kong X,Kong Q

11-0299-42 was used in Flow cytometry/Cell sorting to investigate the potential effects of MenSC-derived mitochondria on ovarian cancer from the perspective of protein expression profiling.

Wed Feb 01 00:00:00 EST 2023

BMP-2 functional polypeptides relieve osteolysis via bi-regulating bone formation and resorption coupled with macrophage polarization.

NPJ Regenerative medicine

Wang J,Xue Y,Wang Y,Liu C,Hu S,Zhao H,Gu Q,Yang H,Huang L,Zhou X,Shi Q

11-0299-42 was used in Flow cytometry/Cell sorting to suggest that BMP2pp may be a potential option for treating prosthetic loosening induced by wear debris from prostheses.

Thu Feb 09 00:00:00 EST 2023

TNF-α activates RELA expression via TNFRSF1B to upregulate OPA1 expression and inhibit chondrogenic differentiation of human adipose stem cells.

Journal of orthopaedic surgery and research

Guo J,Ye W,Wu X,Huang H,Li B,Sun Z,Ren Z,Yang Z

11-0299-42 was used in Flow cytometry/Cell sorting to show that the presence of TNF-α led to increased expression of TNFα receptor 2 (TNFRSF1B) and RELA during chondrogenic differentiation of hADSCs.

Tue Jun 13 00:00:00 EDT 2023

Single-cell analysis of immune cells on gingiva-derived mesenchymal stem cells in experimental autoimmune uveitis.

iScience

Gao Y,Duan R,Li H,Jiang L,Tao T,Liu X,Zhu L,Li Z,Chen B,Zheng S,Lin X,Su W

11-0299-42 was used in Flow cytometry/Cell sorting to generate a lymph node single-cell transcriptomic atlas of GMSC-treated experimental autoimmune uveitis mice.

Fri May 19 00:00:00 EDT 2023

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration.

Cell death and differentiation

Deng CL,Hu CB,Ling ST,Zhao N,Bao LH,Zhou F,Xiong YC,Chen T,Sui BD,Yu XR,Hu CH

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study.

Stem cells international

Mohammed SS,Mansour MF,Salem NA

11-0299-42 was used in Flow cytometry/Cell sorting to evaluate the therapeutic effects of stem cells at histological, molecular, biochemical, and functional levels in a methotrexate-induced testicular damage model.

Fri Nov 05 00:00:00 EDT 2021

Integrin alpha-V is an important driver in pancreatic adenocarcinoma progression.

Journal of experimental & clinical cancer research : CR

Kemper M,Schiecke A,Maar H,Nikulin S,Poloznikov A,Galatenko V,Tachezy M,Gebauer F,Lange T,Riecken K,Tonevitsky A,Aigner A,Izbicki J,Schumacher U,Wicklein D

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

Integrin alpha-V is an important driver in pancreatic adenocarcinoma progression.

Journal of experimental & clinical cancer research : CR

Kemper M,Schiecke A,Maar H,Nikulin S,Poloznikov A,Galatenko V,Tachezy M,Gebauer F,Lange T,Riecken K,Tonevitsky A,Aigner A,Izbicki J,Schumacher U,Wicklein D

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

11-0299 was used in Flow cytometry/Cell sorting to demonstrate that endogenous activation of the PAX7 transcription factor results in stable epigenetic remodeling and differentiates human pluripotent stem cells into skeletal myogenic progenitor cells.

Tue May 12 00:00:00 EDT 2020

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

Tue May 12 00:00:00 EDT 2020

Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury.

Stem cell research & therapy

Sierra-Parraga JM,Merino A,Eijken M,Leuvenink H,Ploeg R,Møller BK,Jespersen B,Baan CC,Hoogduijn MJ

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Wed Aug 12 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

11-0299-42 was used in Flow cytometry/Cell sorting to investigate the role of Pluronic F-127 (PF-127) hydrogel plus antioxidant sodium ascorbyl phosphate (SAP) in enhancing Wharton's jelly mesenchymal stem cell (WJMSC)-mediated effectiveness on full-thickness skin wound healing in mice.

Fri Apr 03 00:00:00 EDT 2020

Phenotype changes of oral epithelial stem cells after in vitro culture.

Brazilian oral research

Daltoe FP,Oliveira NAJ,Peron CN,Sharpe PT,Mantesso A

11-0299 was used in Flow cytometry/Cell sorting to suggest that phenotype changes may be part of an intrinsic cellular mechanism to conserve levels of protein expression as they may found in the human body.

Thu Apr 23 00:00:00 EDT 2020

hADSCs derived extracellular vesicles inhibit NLRP3inflammasome activation and dry eye.

Scientific reports

Yu C,Chen P,Xu J,Liu Y,Li H,Wang L,Di G

11-0299 was used in Flow cytometry/Cell sorting to address the therapeutic efficacy of human adipose tissue stem cells derived extracellular vesicles (hADSC-Evs) in a mouse model of dry eye disease and to investigate the underlying mechanisms involved.

Thu Sep 03 00:00:00 EDT 2020

hADSCs derived extracellular vesicles inhibit NLRP3inflammasome activation and dry eye.

Scientific reports

Yu C,Chen P,Xu J,Liu Y,Li H,Wang L,Di G

Thu Sep 03 00:00:00 EDT 2020

Basic fibroblast growth factor enhances proliferation and hepatocyte growth factor expression of feline mesenchymal stem cells.

Regenerative therapy

Fujimoto Y,Yokozeki T,Yokoyama A,Tabata Y

11-0299-42 was used in Flow Cytometry to evaluate the effect of basic fibroblast growth factor (bFGF) on the proliferation and secretion activity of feline adipose-derived mesenchymal stem cells (MSC).

Tue Dec 01 00:00:00 EST 2020

High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment.

Biology open

Sun Y,Ren Y,Yang F,He Y,Liang S,Guan L,Cheng F,Liu Y,Lin J

11-0299 was used in Flow cytometry/Cell sorting to determine whether primary MenSCs exist in the sedimentation of the deciduous endometrium after DGC and further to evaluate the isolation of MenSCs by direct red blood cell lysis treatment.

Thu May 02 00:00:00 EDT 2019

High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment.

Biology open

Sun Y,Ren Y,Yang F,He Y,Liang S,Guan L,Cheng F,Liu Y,Lin J

Thu May 02 00:00:00 EDT 2019

The suppressive effects of miR-508-5p on the odontogenic differentiation of human dental pulp stem cells by targeting glycoprotein non-metastatic melanomal protein B.

Stem cell research & therapy

Liu F,Wang X,Yang Y,Hu R,Wang W,Wang Y

11-0299-42 was used in Flow cytometry/Cell sorting to investigate the effects of miR-508-5p on the glycoprotein non-metastatic melanomal protein B expression and the odontogenic differentiation of human dental pulp stem cells.

Tue Jan 22 00:00:00 EST 2019

Investigating the potential of the secretome of mesenchymal stem cells derived from sickle cell disease patients.

PloS one

Ribeiro TO,Silveira BM,Meira MC,Carreira ACO,Sogayar MC,Meyer R,Fortuna V

11-0299 was used in Flow cytometry/Cell sorting to find that hypoxically conditioned cells presented increased expression and secretion of several well-characterized trophic growth factors (VEGF, IL8, MCP-1, ANG) directly linked to angiogenesis and tissue repair.

Wed Mar 11 00:00:00 EDT 2020

Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model.

Stem cells international

Yoon D,Yoon D,Sim H,Hwang I,Lee JS,Chun W

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Exosomes secreted by adipose-derived mesenchymal stem cells regulate type I collagen metabolism in fibroblasts from women with stress urinary incontinence.

Stem cell research & therapy

Liu X,Wang S,Wu S,Hao Q,Li Y,Guo Z,Wang W

11-0299 was used in Flow cytometry/Cell sorting to evaluate the effects of exomes secreted by adipose-derived mesenchymal stem cells on collagen metabolism in cultured fibroblasts from women with stress urinary incontinence.

Wed Jun 13 00:00:00 EDT 2018

The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries.

Nature communications

Grasso S,Chapelle J,Salemme V,Aramu S,Russo I,Vitale N,Verdun di Cantogno L,Dallaglio K,Castellano I,Amici A,Centonze G,Sharma N,Lunardi S,Cabodi S,Cavallo F,Lamolinara A,Stramucci L,Moiso E,Provero P,Albini A,Sapino A,Staaf J,Di Fiore PP,Bertalot G,Pece S,Tosoni D,Confalonieri S,Iezzi M,Di Stefano P,Turco E,Defilippi P

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Immunofluorescence

Thu Mar 16 00:00:00 EDT 2017

Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro.

Human reproduction (Oxford, England)

Green CJ,Fraser ST,Day ML

11-0299 was used in Flow cytometry/Cell sorting to study an increase in apical fibronectin in blastocysts mediated by insulin-like growth factor to increase blastocyst attachment to endometrial epithelial cells in vitro.

Sun Feb 01 00:00:00 EST 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Sat May 09 00:00:00 EDT 2015

Efficacy of autologous stem cell-based therapy for osteonecrosis of the femoral head in sickle cell disease: a five-year follow-up study.

Stem cell research & therapy

Daltro GC,Fortuna V,de Souza ES,Salles MM,Carreira AC,Meyer R,Freire SM,Borojevic R

11-0299 was used in Flow cytometry/Cell sorting to report a significant improvement in clinical joint symptoms and pain relief in patients after bone marrow-derived mononuclear cell implantation.

Fri May 29 00:00:00 EDT 2015

Differentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable three-dimensional scaffolds.

Tissue engineering. Part A

Gao S,Zhao P,Lin C,Sun Y,Wang Y,Zhou Z,Yang D,Wang X,Xu H,Zhou F,Cao L,Zhou W,Ning K,Chen X,Xu J

11-0299 was used in Immunocytochemistry to investigate the differentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable 3D scaffolds.

Tue Apr 01 00:00:00 EDT 2014

PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.

International journal of clinical and experimental pathology

Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Tue May 26 00:00:00 EDT 2015

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 11-0299-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.

Stem cells (Dayton, Ohio)

Gassenmaier M,Chen D,Buchner A,Henkel L,Schiemann M,Mack B,Schendel DJ,Zimmermann W,Pohla H

11-0299 was used in Flow cytometry/Cell sorting to study the expression of CXC chemokine receptor 4 in a subpopulation of tumour-initiating cells in renal cell carcinoma cell lines and study its role in their maintenance.

Thu Aug 01 00:00:00 EDT 2013

Downregulation of Mcl-1 synergizes the apoptotic response to combined treatment with cisplatin and a novel fiber chimeric oncolytic adenovirus.

Oncology reports

You L,Wang Y,Jin Y,Qian W

11-0299 was used in Flow cytometry/Cell sorting to determine how SG511 sensitises cancer cells to apoptosis by reducing anti-apoptotic Mcl-1 protein levels.

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; very late activation protein, beta polypeptide; VLA-4 subunit beta

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Gene Aliases: CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

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UniProt ID: (Human) P05556

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Entrez Gene ID: (Human) 3688

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Function(s)

virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion integrin

Process(es)

Performance Guarantee

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CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), FITC, eBioscience™

CD29 (Integrin beta 1) Antibody (11-0299-42) in Flow

Product Details

11-0299-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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