Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), eBioscience™

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

18 Published Figures

27 References

View all (125) Integrin beta 1 (CD29) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD29 (Integrin beta 1) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

CD29 (Integrin beta 1) Antibody (14-0299-82) in ICC/IF

Immunofluorescence analysis of CD29 (Integrin beta 1) was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), eBioscience™ (Product # 14-0299-80) at 1:1... View More

CD29 (Integrin beta 1) Antibody in Western Blot (WB)

CD29 (Integrin beta 1) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

14-0299-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Bovine, Dog, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

TS2/16

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210468

Product Specific Information

Description: The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta 1 integrins.

Applications Reported: This TS2/16 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: This TS2/16 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human osteoarthritic articular cartilage stem cells suppress osteoclasts and improve subchondral bone remodeling in experimental knee osteoarthritis partially by releasing TNFAIP3.

Stem cell research & therapy

Li ZL,Li XT,Hao RC,Wang FY,Wang YX,Zhao ZD,Li PL,Yin BF,Mao N,Ding L,Zhu H

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Wed Sep 27 00:00:00 EDT 2023

Comparative proteomic analysis of the mitochondria of menstrual stem cells and ovarian cancer cells.

Experimental and therapeutic medicine

Chen X,Zhong W,Chang Y,Song T,Liu B,Kong X,Kong Q

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy.

eLife

Li W,Lou X,Zha Y,Qin Y,Zha J,Hong L,Xie Z,Yang S,Wang C,An J,Zhang Z,Qiao S

14-0299-82 was used in Immunohistochemistry (Frozen) to investigate intercellular communication drivers of myocardial fibrosis in mouse heart with high-fat-diet/streptozotocin-induced diabetes at single-cell resolution.

Mon Apr 03 00:00:00 EDT 2023

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A₁₆₅ No Longer Depends on the Growth Factor's Presence.

Biomolecules

Deissler HL,Rehak M,Wolf A

14-0299-82 was used in Western Blotting to conclude that several days after the addition of VEGF-A165 to iBREC, barrier dysfunction is mainly sustained by increased paracellular flow and impaired adhesion.

Mon May 23 00:00:00 EDT 2022

Beovu, but not Lucentis impairs the function of the barrier formed by retinal endothelial cells in vitro.

Scientific reports

Deissler HL,Busch C,Wolf A,Rehak M

14-0299-82 was used in Immunocytochemistry-immunoflourescence to investigate if Beovu changes the behavior of retinal endothelial cells.

Thu Jul 21 00:00:00 EDT 2022

β1 integrin monoclonal antibody treatment ameliorates cerebral cavernous malformations.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

McCurdy S,Lin J,Shenkar R,Moore T,Lightle R,Faurobert E,Lopez-Ramirez MA,Awad I,Ginsberg MH

14-0299-82 was used in Western Blotting to conclude that targeting β1 integrin with an activated-specific antibody reduces acute murine CCM lesion development, which was found to be associated with suppression of endothelial ROCK activity.

Thu Dec 01 00:00:00 EST 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

Chromatin condensation regulates endothelial cell adaptation to shear stress.

Molecular biology of the cell

Danielsson BE,Tieu KV,Spagnol ST,Vu KK,Cabe JI,Raisch TB,Dahl KN,Conway DE

14-0299-82 was used in Immunocytochemistry-immunofluorescence to indicate that chromatin condensation, and potentially by extension nuclear stiffness, is an important aspect of EC adaptation to FSS.

Thu Sep 15 00:00:00 EDT 2022

Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration.

Cell death and differentiation

Deng CL,Hu CB,Ling ST,Zhao N,Bao LH,Zhou F,Xiong YC,Chen T,Sui BD,Yu XR,Hu CH

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes.

American journal of physiology. Lung cellular and molecular physiology

Wu Y,Huang Y,Zhang W,Gunst SJ

14-0299-82 was used in Western Blotting to demonstrate that furin inhibits inflammation in airway smooth muscle induced by IL-13 and that the anti-inflammatory effects of furin are mediated by activating integrin proteins and integrin-associated signaling complexes that regulate Akt-mediated pathways to the nucleus.

Thu Jul 01 00:00:00 EDT 2021

Integrin alpha-V is an important driver in pancreatic adenocarcinoma progression.

Journal of experimental & clinical cancer research : CR

Kemper M,Schiecke A,Maar H,Nikulin S,Poloznikov A,Galatenko V,Tachezy M,Gebauer F,Lange T,Riecken K,Tonevitsky A,Aigner A,Izbicki J,Schumacher U,Wicklein D

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure.

Journal of diabetes research

Jäckle A,Ziemssen F,Kuhn EM,Kampmeier J,Lang GK,Lang GE,Deissler H,Deissler HL

14-0299-82 was used in Immunocytochemistry, Western Blot to evaluate the effects of DPP-4 inhibitors on retinal endothelial cell barrier stability using immortalized endothelial cells of the bovine retina.

Mon Apr 05 00:00:00 EDT 2021

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure.

Journal of diabetes research

Jäckle A,Ziemssen F,Kuhn EM,Kampmeier J,Lang GK,Lang GE,Deissler H,Deissler HL

Mon Apr 05 00:00:00 EDT 2021

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure.

Journal of diabetes research

Jäckle A,Ziemssen F,Kuhn EM,Kampmeier J,Lang GK,Lang GE,Deissler H,Deissler HL

Mon Apr 05 00:00:00 EDT 2021

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure.

Journal of diabetes research

Jäckle A,Ziemssen F,Kuhn EM,Kampmeier J,Lang GK,Lang GE,Deissler H,Deissler HL

Mon Apr 05 00:00:00 EDT 2021

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Tue May 12 00:00:00 EDT 2020

Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury.

Stem cell research & therapy

Sierra-Parraga JM,Merino A,Eijken M,Leuvenink H,Ploeg R,Møller BK,Jespersen B,Baan CC,Hoogduijn MJ

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Wed Aug 12 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

hADSCs derived extracellular vesicles inhibit NLRP3inflammasome activation and dry eye.

Scientific reports

Yu C,Chen P,Xu J,Liu Y,Li H,Wang L,Di G

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2020

Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model.

Stem cells international

Yoon D,Yoon D,Sim H,Hwang I,Lee JS,Chun W

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries.

Nature communications

Grasso S,Chapelle J,Salemme V,Aramu S,Russo I,Vitale N,Verdun di Cantogno L,Dallaglio K,Castellano I,Amici A,Centonze G,Sharma N,Lunardi S,Cabodi S,Cavallo F,Lamolinara A,Stramucci L,Moiso E,Provero P,Albini A,Sapino A,Staaf J,Di Fiore PP,Bertalot G,Pece S,Tosoni D,Confalonieri S,Iezzi M,Di Stefano P,Turco E,Defilippi P

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Immunofluorescence

Thu Mar 16 00:00:00 EDT 2017

Tumor-penetrating iRGD peptide inhibits metastasis.

Molecular cancer therapeutics

Sugahara KN,Braun GB,de Mendoza TH,Kotamraju VR,French RP,Lowy AM,Teesalu T,Ruoslahti E

14-0299 was used in Flow cytometry/Cell sorting to investigate the anti-metastatic activity of tumor-penetrating iRGD peptide.

Thu Jan 01 00:00:00 EST 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Sat May 09 00:00:00 EDT 2015

PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.

International journal of clinical and experimental pathology

Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Tue May 26 00:00:00 EDT 2015

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD29 (Integrin beta 1) monoclonal antibody (Product # 14-0299-82) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Annexin A2 regulates β1 integrin internalization and intestinal epithelial cell migration.

The Journal of biological chemistry

Rankin CR,Hilgarth RS,Leoni G,Kwon M,Den Beste KA,Parkos CA,Nusrat A

14-0299 was used in Functional assays to suggest that AnxA2 has an important role in controlling the dynamics of β1 integrin at the cell surface.

Fri May 24 00:00:00 EDT 2013

GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.

PloS one

Fekete N,Rojewski MT,Fürst D,Kreja L,Ignatius A,Dausend J,Schrezenmeier H

14-0299 was used in Flow cytometry/Cell sorting to investigate methods for animal-free expansion of bone marrow-derived mesenchymal stem cells.

Mon Feb 11 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; very late activation protein, beta polypeptide; VLA-4 subunit beta

View more View less

Gene Aliases: CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

View more View less

UniProt ID: (Human) P05556

View more View less

Entrez Gene ID: (Human) 3688

View more View less

Function(s)

virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), eBioscience™

CD29 (Integrin beta 1) Antibody (14-0299-82) in ICC/IF

Product Details

14-0299-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!