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Invitrogen
CD294 (CRTH2) Monoclonal Antibody (BM16), PE, eBioscience™
FIGURE: 1 / 1
CD294 (CRTH2) Antibody (12-2949-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or Anti-Human CD294 (CRTH2) PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-2949-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
BM16
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572601
Product Specific Information
Description: This BM16 monoclonal antibody reacts with human CD294, which is also known as G protein-coupled receptor-44 (GPR-44) or chemoattractant receptor expressed on Th2 cells (CRTH2). CD294 is expressed on Th2 cells, some CD8+ cells, eosinophils, and basophils. Moreover, CD294 binds prostaglandin D2, which promotes leukocyte chemotaxis during allergic responses. CD294 has been reported to mediate prostaglandin D2-mediated suppression of apoptosis of Th2 cells by activating the PI3K pathway.
Applications Reported: This BM16 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This BM16 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. At least two different isoforms of CRTH2 are known to exist. Single nucleotide polymorphisms in the 3' UTR of the CRTH2 gene have been associated with asthma susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD294; chemoattractant receptor homologous molecule expressed on T helper type 2 cells; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G protein-coupled receptor 44; G-protein coupled receptor 44; MGC130436; Prostaglandin D2 receptor 2; putative G-protein coupled receptor 44
Gene Aliases: CD294; CRTH2; DL1R; DP2; GPR44; PTGDR2
UniProt ID: (Human) Q9Y5Y4
Entrez Gene ID: (Human) 11251
chemotaxis
immune response
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
calcium-mediated signaling
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of male germ cell proliferation
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