Invitrogen

CD304 (Neuropilin-1) Monoclonal Antibody (3DS304M), Super Bright™ 780, eBioscience™

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View all (50) Neuropilin 1 (CD304) antibodies
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Protocols
Questions & Answers
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Product Details

78-3041-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
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Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3DS304M

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802475

Product Specific Information

Description: The monoclonal antibody 3DS304M recognizes mouse CD304 (Neuropilin-1), a type 1 transmembrane glycoprotein present on the surface of multiple cell types, including: regulatory T cells (Treg), NKT cells, DC, certain types of stem cells, neurons, endothelial cells, and some neoplastic cells. Neuropilin-1, in complex with plexins, serves as a co-receptor for type 3 semaphorins in growing neurons. It is also involved in the process of angiogenesis being a part of a functional receptor for VEGF in endothelial cells. In mice, Neuropilin-1 is expressed on thymus-derived natural Treg but not peripherally induced Treg. In addition, transient expression on recently activated non-regulatory T cells has been observed. Neuropilin-1 has been used as a marker of recent thymic emigrants in the mouse iNKT cell population. It has been shown that Neuropilin-1 forms a complex with TGF beta receptors, activating the latent form of TGF beta (LAP-TGF beta 1) and augmenting canonical Smad2/3 signaling.

This 3DS304M antibody will recognize formaldehyde-fixed as well as methanol-treated epitopes.

Applications Reported: This 3DS304M antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3DS304M antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A5 protein; CD304; Neuropilin; Neuropilin-1; Neuropilin-1 precursor (A5 protein); Neuropilin1; NRP 1; sNRP 1; sNRP1; soluble NRP 1; soluble NRP1; transmembrane receptor

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Gene Aliases: C530029I03; NP-1; NPN-1; Npn1; Nrp; Nrp1

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UniProt ID: (Mouse) P97333

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Entrez Gene ID: (Mouse) 18186

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Function(s)
vascular endothelial growth factor-activated receptor activity protein binding heparin binding semaphorin receptor activity growth factor binding vascular endothelial growth factor binding metal ion binding oxidoreductase
Process(es)
angiogenesis patterning of blood vessels neuron migration sprouting angiogenesis cell migration involved in sprouting angiogenesis multicellular organism development nervous system development axon guidance axonal fasciculation heart development motor neuron axon guidance organ morphogenesis dendrite development cell migration peptidyl-tyrosine phosphorylation facial nerve structural organization trigeminal nerve morphogenesis trigeminal nerve structural organization vestibulocochlear nerve structural organization nerve development branchiomotor neuron axon guidance gonadotrophin-releasing hormone neuronal migration to the hypothalamus cell differentiation negative regulation of axon extension retinal ganglion cell axon guidance cellular response to hepatocyte growth factor stimulus endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus ventral trunk neural crest cell migration vascular endothelial growth factor signaling pathway VEGF-activated neuropilin signaling pathway otic placode formation negative regulation of neuron apoptotic process platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway hepatocyte growth factor receptor signaling pathway sympathetic nervous system development neuron development regulation of axon extension involved in axon guidance positive regulation of axon extension involved in axon guidance negative regulation of axon extension involved in axon guidance artery morphogenesis axon extension involved in axon guidance positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis axonogenesis involved in innervation dichotomous subdivision of terminal units involved in salivary gland branching angiogenesis involved in coronary vascular morphogenesis cell migration involved in coronary vasculogenesis coronary artery morphogenesis retina vasculature morphogenesis in camera-type eye renal artery morphogenesis sympathetic ganglion development trigeminal ganglion development ganglion morphogenesis positive regulation of ERK1 and ERK2 cascade semaphorin-plexin signaling pathway commissural neuron axon guidance regulation of retinal ganglion cell axon guidance sensory neuron axon guidance sympathetic neuron projection extension sympathetic neuron projection guidance neural crest cell migration involved in autonomic nervous system development toxin transport semaphorin-plexin signaling pathway involved in neuron projection guidance semaphorin-plexin signaling pathway involved in axon guidance positive regulation of retinal ganglion cell axon guidance VEGF-activated neuropilin signaling pathway involved in axon guidance protein localization to early endosome facioacoustic ganglion development negative regulation of extrinsic apoptotic signaling pathway
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