Invitrogen

CD31 Monoclonal Antibody (WM59)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

7 References

View all (297) CD31 (PECAM-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD31 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CD31 Antibody (MA1-26196) in ICC/IF

Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (WM59) (Product # MA1-26196) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary An... View More

CD31 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD31 Monoclonal Antibody (WM59) (Product # MA1-26196) was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 (Ref:https://www.nature.com/articles/onc2013214). {RE}

Product Details

MA1-26196

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunohistochemistry - Free Floating (IHC (Free))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:50-1:100

Flow Cytometry (Flow)

1:50-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

Published species

Mouse, Rabbit, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

WM59

Immunogen

Human CD31

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_779679

Product Specific Information

In FACS use 10 µL of the suggested working dilution to label 10^6 cels in 100 µL.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial cell-derived MMP19 promotes pulmonary fibrosis by inducing E(nd)MT and monocyte infiltration.

Cell communication and signaling : CCS

Zhao W,Wang L,Yang J,Chen X,Guo X,Xu K,Wang N,Zhao W,Xia C,Lian H,Rosas I,Yu G

MA1-26196 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation to find that MMP19 expression was significantly increased in the lung endothelial cells of IPF patients and BLM-induced mice compared to the control groups.

Mon Mar 13 00:00:00 EDT 2023

An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis.

Nature communications

Lu G,Xu Y,Liu Q,Chen M,Sun H,Wang P,Li X,Wang Y,Li X,Hui X,Luo E,Liu J,Jiang Q,Liang J,Fan Y,Sun Y,Zhang X

MA1-26196 was used in Immunohistochemistry to design an instantly fixable and self-adaptive scaffold by dopamine-modified hyaluronic acid chelating Ca2+ of the microhydroxyapatite surface and bonding type I collagen to highly simulate the natural bony matrix.

Fri May 06 00:00:00 EDT 2022

Micro-anatomic alterations of the placenta in a non-human primate model of gestational protein-restriction.

PloS one

Sargent J,Roberts V,D'Souza K,Wright A,Gaffney J,Frias A

MA1-26196 was used in Immunohistochemistry to histologically characterize the micro-anatomic alterations associated with adverse pregnancy outcomes taking an approach that permits investigation of the 3D vascular structure and surrounding histology without the requirement for 3D vascular casting or relying on 2D stereology which both have methodological limitations.

Tue Sep 15 00:00:00 EDT 2020

Clarification and confocal imaging of the nonhuman primate placental micro-anatomy.

BioTechniques

Sargent JA,Roberts V,Gaffney JE,Frias AE

Published figure using CD31 monoclonal antibody (Product # MA1-26196) in Immunohistochemistry

Fri Feb 01 00:00:00 EST 2019

Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers.

BMC cancer

Ghebeh H,Sleiman GM,Manogaran PS,Al-Mazrou A,Barhoush E,Al-Mohanna FH,Tulbah A,Al-Faqeeh K,Adra CN

MA1-26196 was used in flow cytometry to determine the phenotype and function of stem/progenitor cells in normal mammary epithelial cell populations and their malignant counterparts

Fri Jun 14 00:00:00 EDT 2013

Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes.

Biomaterials

Alcala MA,Shade CM,Uh H,Kwan SY,Bischof M,Thompson ZP,Gogick KA,Meier AR,Strein TG,Bartlett DL,Modzelewski RA,Lee YJ,Petoud S,Brown CK

MA1-26196 was used in immunohistochemistry to study the use of luminescent dendrimer lanthanide complexes for in vivo tumor imaging

Thu Dec 01 00:00:00 EST 2011

Luminescence targeting and imaging using a nanoscale generation 3 dendrimer in an in vivo colorectal metastatic rat model.

Nanomedicine : nanotechnology, biology, and medicine

Alcala MA,Kwan SY,Shade CM,Lang M,Uh H,Wang M,Weber SG,Bartlett DL,Petoud S,Lee YJ

MA1-26196 was used in immunohistochemistry to study the application of generation 3 polyamidoamine 4-amino-1,8-naphthalimide dendrimer containing 8 europium ions (Eu-G3P4A18N) for luminescence imaging

Wed Jun 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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Gene Aliases: C85791; CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; Pecam; PECAM-1; PECAM1

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UniProt ID: (Human) P16284, (Mouse) Q08481

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Entrez Gene ID: (Rhesus monkey) 718302, (Cynomolgus monkey) 102117792, (Human) 5175, (Mouse) 18613

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD31 Monoclonal Antibody (WM59)

CD31 Antibody (MA1-26196) in ICC/IF

Product Details

MA1-26196

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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