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Invitrogen
CD32 Monoclonal Antibody (6C4 (CD32)), Functional Grade, eBioscience™
FIGURE: 1 / 3
CD32 Antibody (16-0329-81) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and 0.5 µg of Mouse IgG1 K Isotype Control Functional Grade Purified (Product # 16-4714-82) (left) or 0.5 µg of Anti-Human CD32 Functional Grade Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82).Cells in the lymphocyte gate were used for analysis.
Product Details
16-0329-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
6C4 (CD32)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1311188
Product Specific Information
Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.
Applications Tested: This 6C4 (CD32) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD32; CD32 receptor 2; CD32 receptor 2a; CDw32; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma receptor IIa; Fc gamma receptor IIb; Fc gamma receptor IIC; Fc gamma RIIb; Fc-gamma RII-a; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma-RIIa; Fc-gamma-RIIb; Fc-gamma-RIIc; fcRII-a; fcRII-b; FGFR2B; IgG Fc receptor II-a; IgG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c; MGC23887; MGC30032; RP11-5K23.6
Gene Aliases: CD32; CD32A; CD32B; CD32C; CDW32; FCG2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FCRIIC; IGFR2
UniProt ID: (Human) P12318, (Human) P31994, (Human) P31995
Entrez Gene ID: (Human) 2212, (Human) 2213, (Human) 9103
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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