Invitrogen

CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor™ 488, eBioscience™

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Antibody Testing Data (2)
Published Figures (42)

CD324 (E-Cadherin) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 44

CD324 (E-Cadherin) Antibody (53-3249-82) in ICC/IF

Immunofluorescence analysis of E-cadherin was performed using 90% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1) (Product # 53-3249-80) at 5 µg/mL in 0.1% BSA, incubated at 4 d... View More

CD324 (E-Cadherin) Antibody in Neutralization (Neu)

CD324 (E-Cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD324 (E-Cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD324 (E-Cadherin) Antibody in Immunohistochemistry (IHC)

CD324 (E-Cadherin) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

CD324 (E-Cadherin) Antibody in Western Blot (WB)

CD324 (E-Cadherin) Antibody in Flow Cytometry (Flow)

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Product Details

53-3249-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

View 14 publications 14 publications

Flow Cytometry (Flow)

Assay-Dependent

View 14 publications 14 publications

Neutralization (Neu)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DECMA-1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671003

Product Specific Information

Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.

Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.

E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.

The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.

Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

Applications Tested: This DECMA-1 antibody has been tested by immunocytochemistry on formaldehyde fixed and permeabilized MDCK cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative.

Bioengineering & translational medicine

Xu ZY,Huang JJ,Liu Y,Chen CW,Qu GW,Wang GF,Zhao Y,Wu XW,Ren JA

53-3249-82 was used in Immunocytochemistry-immunofluorescence to show that the development of such photo-responsive bioink has the potential to replace tumor-derived Matrigel and facilitate the application of organoids in translational medicine and disease modeling.

Sun Jan 01 00:00:00 EST 2023

Involvement of Substance P (SP) and Its Related NK1 Receptor in Primary Sjögren's Syndrome (pSS) Pathogenesis.

Cells

Rosso P,Fico E,Colafrancesco S,Bellizzi MG,Priori R,Cerbelli B,Leopizzi M,Giordano C,Greco A,Tirassa P,Severini C,Fusconi M

53-3249-82 was used in Immunohistochemistry to examine the expression levels of substance P (SP) and its preferred G protein-coupled TK Receptor 1 (NK1R) and apoptosis markers in biopsies of the minor salivary gland (MSG) from pSS patients compared with patients with idiopathic sicca syndrome.

Tue May 09 00:00:00 EDT 2023

Differentiated mouse kidney tubuloids as a novel in vitro model to study collecting duct physiology.

Frontiers in cell and developmental biology

Olde Hanhof CJA,Dilmen E,Yousef Yengej FA,Latta F,Ammerlaan CME,Schreurs J,Hooijmaijers L,Jansen J,Rookmaaker MB,Orhon I,Verhaar MC,Hoenderop JG

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry (PFA fixed)

Sat Feb 11 00:00:00 EST 2023

Aspirin increases the efficacy of gemcitabine in pancreatic cancer by modulating the PI3K/AKT/mTOR signaling pathway and reversing epithelial‑mesenchymal transition.

Oncology letters

Zhou H,Yun X,Shu Y,Xu K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Western Blot

Wed Mar 01 00:00:00 EST 2023

Disentangling cadherin-mediated cell-cell interactions in collective cancer cell migration.

Biophysical journal

Zisis T,Brückner DB,Brandstätter T,Siow WX,d'Alessandro J,Vollmar AM,Broedersz CP,Zahler S

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Neutralization

Tue Jan 04 00:00:00 EST 2022

CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway.

Molecular medicine reports

Li L,Hu Y,Chen D,Zhu J,Bao W,Xu X,Chen H,Chen W,Feng R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Sat Jan 01 00:00:00 EST 2022

Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis.

Cancer research communications

Takeuchi A,Asano N,Imatani A,Saito M,Jin X,Saito M,Kanno T,Hatta W,Uno K,Koike T,Masamune A

53-3249-82 was used in Immunohistochemistry-immunofluorescence to provide an insight into the mechanism involved in aging-related gastric carcinogenesis through studies utilizing organoids established from young and aged murine stomachs.

Mon Aug 01 00:00:00 EDT 2022

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Western Blot

Fri Dec 31 00:00:00 EST 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Western Blot

Thu Apr 01 00:00:00 EDT 2021

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

International journal of chronic obstructive pulmonary disease

Fujioka N,Kitabatake M,Ouji-Sageshima N,Ibaraki T,Kumamoto M,Fujita Y,Hontsu S,Yamauchi M,Yoshikawa M,Muro S,Ito T

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

International journal of chronic obstructive pulmonary disease

Fujioka N,Kitabatake M,Ouji-Sageshima N,Ibaraki T,Kumamoto M,Fujita Y,Hontsu S,Yamauchi M,Yoshikawa M,Muro S,Ito T

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor.

International journal of molecular sciences

Troitskaya O,Novak D,Nushtaeva A,Savinkova M,Varlamov M,Ermakov M,Richter V,Koval O

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Mon Nov 29 00:00:00 EST 2021

Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis.

International journal of molecular sciences

Hsu SH,Chou LF,Hong CH,Chang MY,Tsai CY,Tian YC,Yang HY,Yang CW

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Neutralization

Sat Dec 04 00:00:00 EST 2021

Opposing effects of Wnt/β-catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea.

Development (Cambridge, England)

Billings SE,Myers NM,Quiruz L,Cheng AG

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry

Tue Jun 01 00:00:00 EDT 2021

Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice.

Pharmaceutics

Oyama N,Kawaguchi M,Itaka K,Kawakami S

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry (PFA fixed)

Fri Oct 29 00:00:00 EDT 2021

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

53-3249 was used in Flow cytometry/Cell sorting to show that VEGFA from the epithelium is required for a distinct endothelial cell (EC) population in the mouse lung.

Mon Mar 09 00:00:00 EDT 2020

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

53-3249 was used in Flow cytometry/Cell sorting to show that VEGFA from the epithelium is required for a distinct endothelial cell (EC) population in the mouse lung.

Mon Mar 09 00:00:00 EDT 2020

Redundant and additive functions of the four Lef/Tcf transcription factors in lung epithelial progenitors.

Proceedings of the National Academy of Sciences of the United States of America

Gerner-Mauro KN,Akiyama H,Chen J

53-3249 was used in Flow cytometry/Cell sorting to generate two quadruple conditional knockouts, four triple mutants, and various combinations of double mutants, showing that the four Lef/Tcf genes function redundantly in the presence of at least two Lef/Tcf paralogs, but additively upon losing additional paralogs to specify and maintain lung epithelial progenitors., using the developing mouse lung as a model system.

Tue Jun 02 00:00:00 EDT 2020

Quantitative single-cell interactomes in normal and virus-infected mouse lungs.

Disease models & mechanisms

Cain MP,Hernandez BJ,Chen J

53-3249 was used in Flow cytometry/Cell sorting to describe a quantitative intuitive algorithm, coupled with an optimized experimental protocol, to construct and compare interactomes as a Control and Sendai virus-infected mouse lungs.

Fri Jun 26 00:00:00 EDT 2020

GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD.

Journal of cell science

Dixon EE,Maxim DS,Halperin Kuhns VL,Lane-Harris AC,Outeda P,Ewald AJ,Watnick TJ,Welling PA,Woodward OM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Thu Jul 16 00:00:00 EDT 2020

Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA).

International journal of molecular sciences

Kim JW,Mahiddine FY,Kim GA

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Western Blot

Wed Nov 25 00:00:00 EST 2020

CD44 Can Compensate for IgSF11 Deficiency by Associating with the Scaffold Protein PSD-95 during Osteoclast Differentiation.

International journal of molecular sciences

Kim H,Takegahara N,Walsh MC,Choi Y

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry

Fri Apr 10 00:00:00 EDT 2020

Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis.

Oncogene

Flood B,Manils J,Nulty C,Flis E,Kenealy S,Barber G,Fay J,Mills KHG,Kay EW,Creagh EM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry

Mon Apr 01 00:00:00 EDT 2019

Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.

Nature communications

Malik V,Glaser LV,Zimmer D,Velychko S,Weng M,Holzner M,Arend M,Chen Y,Srivastava Y,Veerapandian V,Shah Z,Esteban MA,Wang H,Chen J,Schöler HR,Hutchins AP,Meijsing SH,Pott S,Jauch R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Fri Aug 02 00:00:00 EDT 2019

Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT.

Cell reports

Bornes L,van Scheppingen RH,Beerling E,Schelfhorst T,Ellenbroek SIJ,Seinstra D,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Tue Nov 26 00:00:00 EST 2019

Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.

Cell reports

Tran KA,Pietrzak SJ,Zaidan NZ,Siahpirani AF,McCalla SG,Zhou AS,Iyer G,Roy S,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Tue May 07 00:00:00 EDT 2019

Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.

Cell reports

Tran KA,Pietrzak SJ,Zaidan NZ,Siahpirani AF,McCalla SG,Zhou AS,Iyer G,Roy S,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Tue May 07 00:00:00 EDT 2019

β-Catenin maintains lung epithelial progenitors after lung specification.

Development (Cambridge, England)

Ostrin EJ,Little DR,Gerner-Mauro KN,Sumner EA,Ríos-Corzo R,Ambrosio E,Holt SE,Forcioli-Conti N,Akiyama H,Hanash SM,Kimura S,Huang SXL,Chen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry

Fri Mar 09 00:00:00 EST 2018

Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.

eLife

Chua CW,Epsi NJ,Leung EY,Xuan S,Lei M,Li BI,Bergren SK,Hibshoosh H,Mitrofanova A,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunofluorescence

Mon Jan 15 00:00:00 EST 2018

Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.

eLife

Chua CW,Epsi NJ,Leung EY,Xuan S,Lei M,Li BI,Bergren SK,Hibshoosh H,Mitrofanova A,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunofluorescence

Mon Jan 15 00:00:00 EST 2018

Brushed nasal epithelial cells are a surrogate for bronchial epithelial CFTR studies.

JCI insight

Brewington JJ,Filbrandt ET,LaRosa FJ,Moncivaiz JD,Ostmann AJ,Strecker LM,Clancy JP

53-3249-82 was used in Immunocytochemistry to examine how faithfully brushed human nasal epithelial cells recapitulate human bronchial epithelial cell Cystic fibrosis transmembrane conductance regulator activity.

Thu Jul 12 00:00:00 EDT 2018

Repopulating retinal microglia restore endogenous organization and function under CX3CL1-CX3CR1 regulation.

Science advances

Zhang Y,Zhao L,Wang X,Ma W,Lazere A,Qian HH,Zhang J,Abu-Asab M,Fariss RN,Roger JE,Wong WT

53-3249 was used in Immunohistochemistry to study microglial homeostasis in the adult rat retina, and the regulation of microglial repopulation by CX3CL1-CX3CR1 signalling.

Thu Mar 01 00:00:00 EST 2018

Identity and dynamics of mammary stem cells during branching morphogenesis.

Nature

Scheele CL,Hannezo E,Muraro MJ,Zomer A,Langedijk NS,van Oudenaarden A,Simons BD,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Thu Feb 16 00:00:00 EST 2017

Identity and dynamics of mammary stem cells during branching morphogenesis.

Nature

Scheele CL,Hannezo E,Muraro MJ,Zomer A,Langedijk NS,van Oudenaarden A,Simons BD,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Thu Feb 16 00:00:00 EST 2017

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

53-3249 was used in in vivo experiments to contribute to a comprehensive understanding of cellular events that underlie allergic and inflammatory diseases of the lung.

Wed Apr 05 00:00:00 EDT 2017

NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer.

Nature

Karki R,Man SM,Malireddi RKS,Kesavardhana S,Zhu Q,Burton AR,Sharma BR,Qi X,Pelletier S,Vogel P,Rosenstiel P,Kanneganti TD

53-3249 was used in Immunofluorescence to explore a key role for NLRC3 in mediating protection against colorectal cancer.

Thu Dec 22 00:00:00 EST 2016

Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells.

Genes & development

Wuidart A,Ousset M,Rulands S,Simons BD,Van Keymeulen A,Blanpain C

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis.

The Journal of pathology

Ören B,Urosevic J,Mertens C,Mora J,Guiu M,Gomis RR,Weigert A,Schmid T,Grein S,Brüne B,Jung M

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis.

Nature medicine

Lovisa S,LeBleu VS,Tampe B,Sugimoto H,Vadnagara K,Carstens JL,Wu CC,Hagos Y,Burckhardt BC,Pentcheva-Hoang T,Nischal H,Allison JP,Zeisberg M,Kalluri R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer.

Nature

Zheng X,Carstens JL,Kim J,Scheible M,Kaye J,Sugimoto H,Wu CC,LeBleu VS,Kalluri R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunohistochemistry

Thu Nov 26 00:00:00 EST 2015

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons.

Scientific reports

Wang S,Wang B,Pan N,Fu L,Wang C,Song G,An J,Liu Z,Zhu W,Guan Y,Xu ZQ,Chan P,Chen Z,Zhang YA

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Immunocytochemistry

Wed Mar 18 00:00:00 EDT 2015

Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer.

Cell death & disease

Liang SQ,Marti TM,Dorn P,Froment L,Hall SR,Berezowska S,Kocher G,Schmid RA,Peng RW

53-3249 was used in Immunofluorescence to show that chemoresistance to pemetrexed is associated with a stem cell-like phenotype characterized by an enriched stem cell gene signature.

Thu Jul 16 00:00:00 EDT 2015

Single luminal epithelial progenitors can generate prostate organoids in culture.

Nature cell biology

Chua CW,Shibata M,Lei M,Toivanen R,Barlow LJ,Bergren SK,Badani KK,McKiernan JM,Benson MC,Hibshoosh H,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

Gastroenterology

Liu S,Li N,Yu X,Xiao X,Cheng K,Hu J,Wang J,Zhang D,Cheng S,Liu S

53-3249 was used in Flow cytometry/Cell sorting to demonstrate that the stem cell factor Nanog regulates expression of ICAM-1 in hepatocellular carcinoma cells.

Wed May 01 00:00:00 EDT 2013

Geminin restrains mesendodermal fate acquisition of embryonic stem cells and is associated with antagonism of Wnt signaling and enhanced polycomb-mediated repression.

Stem cells (Dayton, Ohio)

Caronna EA,Patterson ES,Hummert PM,Kroll KL

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 53-3249-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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Gene Aliases: AA960649; Arc-1; Cadherin-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO; Uvomorulin

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UniProt ID: (Dog) F1PAA9, (Human) P12830, (Mouse) P09803

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Entrez Gene ID: (Dog) 442858, (Human) 999, (Mouse) 12550

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Function(s)

protein binding beta-catenin binding ankyrin binding GTPase activating protein binding identical protein binding gamma-catenin binding glycoprotein binding calcium ion binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein phosphatase binding protein domain specific binding metal ion binding cadherin

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules regulation of gene expression negative regulation of cell-cell adhesion negative regulation of cell migration adherens junction organization positive regulation of protein import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development extracellular matrix disassembly extracellular matrix organization neuron projection development response to drug positive regulation of transcription factor import into nucleus establishment of protein localization to plasma membrane in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis cell adhesion sensory perception of sound calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process regulation of protein localization regulation of water loss via skin regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration

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CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor™ 488, eBioscience™

CD324 (E-Cadherin) Antibody (53-3249-82) in ICC/IF

Product Details

53-3249-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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