Invitrogen

CD326 (EpCAM) Monoclonal Antibody (1B7), PE, eBioscience™

19 Published Figures

27 References

View all (275) EpCAM (CD326) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (19)

CD326 (EpCAM) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 20

CD326 (EpCAM) Antibody (12-9326-42) in Flow

Staining of A549 human lung carcinoma cell line with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-82) (blue histogram) or Anti-Human CD326 (EpCAM) PE (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-9326-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 26 publications 26 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1B7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11044497

Product Specific Information

Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.

Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1B7 antibody has been pre-titrated and tested by flow cytometric analysis of A549 human lung carcinoma cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2023

Characteristics of Extracellular Vesicles from a High-Grade Serous Ovarian Cancer Cell Line Derived from a Platinum-Resistant Patient as a Potential Tool for Aiding the Prediction of Responses to Chemotherapy.

Pharmaceuticals (Basel, Switzerland)

Černe K,Kelhar N,Resnik N,Herzog M,Vodnik L,Veranič P,Kobal B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Tue Jun 20 00:00:00 EDT 2023

Dendritic cell phenotype and function in a 3D co-culture model of patient-derived metastatic colorectal cancer organoids.

Frontiers in immunology

Subtil B,Iyer KK,Poel D,Bakkerus L,Gorris MAJ,Escalona JC,van den Dries K,Cambi A,Verheul HMW,de Vries IJM,Tauriello DVF

12-9326-42 was used in Flow cytometry/Cell sorting to examine expression of activation markers in both mature and immature DCs and their ability to activate T cells were impacted by co-culture with tumor organoids using a 3D modelling.

Mon Feb 13 00:00:00 EST 2023

The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay.

Molecular therapy. Methods & clinical development

Hirai H,Liang X,Sun Y,Zhang Y,Zhang J,Chen YE,Mou H,Zhao Y,Xu J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Thu Mar 10 00:00:00 EST 2022

The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay.

Molecular therapy. Methods & clinical development

Hirai H,Liang X,Sun Y,Zhang Y,Zhang J,Chen YE,Mou H,Zhao Y,Xu J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Thu Mar 10 00:00:00 EST 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties.

Cell death & disease

Zhou K,Sun Y,Dong D,Zhao C,Wang W

12-9326-42 was used in Flow cytometry/Cell sorting to indicate that EMP3 is a putative tumor suppressor in breast cancer, and EMP3 downregulation may be responsible for breast cancer chemoresistance.

Sun Sep 12 00:00:00 EDT 2021

EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties.

Cell death & disease

Zhou K,Sun Y,Dong D,Zhao C,Wang W

12-9326-42 was used in Flow cytometry/Cell sorting to indicate that EMP3 is a putative tumor suppressor in breast cancer, and EMP3 downregulation may be responsible for breast cancer chemoresistance.

Sun Sep 12 00:00:00 EDT 2021

Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung.

Nature communications

Tyagi A,Sharma S,Wu K,Wu SY,Xing F,Liu Y,Zhao D,Deshpande RP,D'Agostino RB,Watabe K

12-9326-42 was used in Flow cytometry/Cell sorting to investigate if chronic exposure of nicotine plays a critical role in the formation of pre-metastatic niche within the lungs by recruiting pro-tumor N2-neutrophils.

Wed Jan 20 00:00:00 EST 2021

miR-34a mimic or pre-mir-34a, which is the better option for cancer therapy? KatoIII as a model to study miRNA action in human gastric cancer cells.

Cancer cell international

Jafari N,Abediankenari S,Hossein-Nataj H

12-9326 was used in Flow cytometry/Cell sorting to evaluate the effect of transfected miRNAs on numerous cellular and molecular processes.

Fri Mar 19 00:00:00 EDT 2021

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

12-9326-42 was used in Flow Cytometry to show that FZD5 is a novel target for the development of therapeutic strategies to overcome chemoresistance and prevent recurrence in TNBC.

Sat Dec 12 00:00:00 EST 2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

12-9326-42 was used in Flow Cytometry to show that FZD5 is a novel target for the development of therapeutic strategies to overcome chemoresistance and prevent recurrence in TNBC.

Sat Dec 12 00:00:00 EST 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Cell

Zhao Q,Liu J,Deng H,Ma R,Liao JY,Liang H,Hu J,Li J,Guo Z,Cai J,Xu X,Gao Z,Su S

12-9326-42 was used in Flow cytometry/Cell sorting to identify a mitochondrial circRNA that drives metaflammation and serves as a therapeutic target for NASH.

Thu Oct 01 00:00:00 EDT 2020

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Cell

Zhao Q,Liu J,Deng H,Ma R,Liao JY,Liang H,Hu J,Li J,Guo Z,Cai J,Xu X,Gao Z,Su S

12-9326-42 was used in Flow cytometry/Cell sorting to identify a mitochondrial circRNA that drives metaflammation and serves as a therapeutic target for NASH.

Thu Oct 01 00:00:00 EDT 2020

Human colorectal cancer cells frequently express IgG and display unique Ig repertoire.

World journal of gastrointestinal oncology

Geng ZH,Ye CX,Huang Y,Jiang HP,Ye YJ,Wang S,Zhou Y,Shen ZL,Qiu XY

12-9326 was used in Flow cytometry/Cell sorting to propose that the carcinogenesis of colon cancer may involve the expression of IgG and unique IgH repertoires in cancer cells.

Fri Mar 15 00:00:00 EDT 2019

Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nature communications

Nguyen QH,Pervolarakis N,Blake K,Ma D,Davis RT,James N,Phung AT,Willey E,Kumar R,Jabart E,Driver I,Rock J,Goga A,Khan SA,Lawson DA,Werb Z,Kessenbrock K

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Wed May 23 00:00:00 EDT 2018

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level.

Stem cell reports

Pode-Shakked N,Gershon R,Tam G,Omer D,Gnatek Y,Kanter I,Oriel S,Katz G,Harari-Steinberg O,Kalisky T,Dekel B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2017

A cell transportation solution that preserves live circulating tumor cells in patient blood samples.

BMC cancer

Stefansson S,Adams DL,Ershler WB,Le H,Ho DH

12-9326 was used in Immunohistochemistry to examine the long term viability of human cancer cell lines in a novel sugar-based cell transportation solution.

Fri May 06 00:00:00 EDT 2016

A cell transportation solution that preserves live circulating tumor cells in patient blood samples.

BMC cancer

Stefansson S,Adams DL,Ershler WB,Le H,Ho DH

12-9326 was used in Immunohistochemistry to examine the long term viability of human cancer cell lines in a novel sugar-based cell transportation solution.

Fri May 06 00:00:00 EDT 2016

Clostridium perfringens enterotoxin C-terminal domain labeled to fluorescent dyes for in vivo visualization of micrometastatic chemotherapy-resistant ovarian cancer.

International journal of cancer

Cocco E,Shapiro EM,Gasparrini S,Lopez S,Schwab CL,Bellone S,Bortolomai I,Sumi NJ,Bonazzoli E,Nicoletti R,Deng Y,Saltzman WM,Zeiss CJ,Centritto F,Black JD,Silasi DA,Ratner E,Azodi M,Rutherford TJ,Schwartz PE,Pecorelli S,Santin AD

12-9326 was used in Flow cytometry/Cell sorting to investigate the rate of tumour fluorescence following administration of Clostridium perfringens enterotoxin conjugated with a fluorescent tag.

Tue Dec 01 00:00:00 EST 2015

Solitomab, an epithelial cell adhesion molecule/CD3 bispecific antibody (BiTE), is highly active against primary chemotherapy-resistant ovarian cancer cell lines in vitro and fresh tumor cells ex vivo.

Cancer

English DP,Bellone S,Schwab CL,Roque DM,Lopez S,Bortolomai I,Cocco E,Bonazzoli E,Chatterjee S,Ratner E,Silasi DA,Azodi M,Schwartz PE,Rutherford TJ,Santin AD

12-9326 was used in Flow cytometry/Cell sorting to demonstrate that solitomab may represent a novel, potentially effective targeted agent for the treatment of chemotherapy resistant ovarian cancer patients overexpressing Ep-CAM.

Sun Feb 01 00:00:00 EST 2015

The isolation and characterization of CTC subsets related to breast cancer dormancy.

Scientific reports

Vishnoi M,Peddibhotla S,Yin W,T Scamardo A,George GC,Hong DS,Marchetti D

Thu Dec 03 00:00:00 EST 2015

Solitomab, an EpCAM/CD3 bispecific antibody construct (BiTE®), is highly active against primary uterine and ovarian carcinosarcoma cell lines in vitro.

Journal of experimental & clinical cancer research : CR

Ferrari F,Bellone S,Black J,Schwab CL,Lopez S,Cocco E,Bonazzoli E,Predolini F,Menderes G,Litkouhi B,Ratner E,Silasi DA,Azodi M,Schwartz PE,Santin AD

12-9326 was used in Flow cytometry/Cell sorting to show that Solitomab may represent an effective treatment for patients with recurrent/metastatic and/or chemo-resistant CS overexpressing EpCAM.

Sat Oct 17 00:00:00 EDT 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Loss of plakoglobin promotes decreased cell-cell contact, increased invasion, and breast cancer cell dissemination in vivo.

Breast cancer research : BCR

Holen I,Whitworth J,Nutter F,Evans A,Brown HK,Lefley DV,Barbaric I,Jones M,Ottewell PD

12-9326 was used in Flow cytometry/Cell sorting to demonstrate a functional role for plakoglobin/γ-catenin in the metastatic process.

Fri May 25 00:00:00 EDT 2012

Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.

Stem cells (Dayton, Ohio)

Paik DY,Janzen DM,Schafenacker AM,Velasco VS,Shung MS,Cheng D,Huang J,Witte ON,Memarzadeh S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2012

Slow-cycling therapy-resistant cancer cells.

Stem cells and development

Moore N,Houghton J,Lyle S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 12-9326-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2012

Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.

BMC cancer

Kalinina T,Güngör C,Thieltges S,Möller-Krull M,Penas EM,Wicklein D,Streichert T,Schumacher U,Kalinin V,Simon R,Otto B,Dierlamm J,Schwarzenbach H,Effenberger KE,Bockhorn M,Izbicki JR,Yekebas EF

12-9326 was used in Flow cytometry/Cell sorting to establish and characterize a new human pancreatic cancer cell line to potentially devise a model to investigate the disease and suggest treatment approaches.

Wed Jun 16 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Tumor-associated calcium signal transducer 1

View more View less

Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

View more View less

UniProt ID: (Human) P16422

View more View less

Entrez Gene ID: (Human) 4072

View more View less

Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD326 (EpCAM) Monoclonal Antibody (1B7), PE, eBioscience™

CD326 (EpCAM) Antibody (12-9326-42) in Flow

Product Details

12-9326-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!