Invitrogen

CD32b Monoclonal Antibody (AT130-2), APC, eBioscience™

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CD32b Antibody (17-0321-82) in Flow

Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.25 µg of Anti-Mouse CD32b APC (right). Total viable cells were used for analysis.

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Product Details

17-0321-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AT130-2

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573142

Product Specific Information

Description: The monoclonal antibody AT130-2 reacts with mouse CD32b, also known as Fc-gamma receptor IIb. CD32b is the only inhibitory member of the Fc-gamma receptor family. Immune complex engagement of CD32b results in the phosphorylation of its intracellular ITIM motif. This engagement serves to attenuate B cell receptor (BCR) signaling and other activating signals, reduces proliferation, reduces antibody production, and may also deliver apoptotic signals. CD32b is the only Fc-gamma receptor expressed by B cells. CD32b is also expressed on monocytes, dendritic cells, neutrophils, eosinophils and basophils, as well as B cell progenitors and plasma cells.

AT130-2 has been reported to have agonist activity.

Applications Reported: This AT130-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AT130-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Optimal target saturation of ligand-blocking anti-GITR antibody IBI37G5 dictates FcγR-independent GITR agonism and antitumor activity.

Cell reports. Medicine

Liu H,Wu W,Sun G,Chia T,Cao L,Liu X,Guan J,Fu F,Yao Y,Wu Z,Zhou S,Wang J,Lu J,Kuang Z,Wu M,He L,Shao Z,Wu D,Chen B,Xu W,Wang Z,He K

17-0321-82 was used in Flow cytometry/Cell sorting to propose an alternative dose-finding strategy that does not rely on the traditional maximal tolerated dose (MTD)-based paradigm but instead on utilizing the RO-function relations as biomarker to guide the clinical translation of GITR and similar co-stimulatory agonists.

Tue Jun 21 00:00:00 EDT 2022

Cells of the human intestinal tract mapped across space and time.

Nature

Elmentaite R,Kumasaka N,Roberts K,Fleming A,Dann E,King HW,Kleshchevnikov V,Dabrowska M,Pritchard S,Bolt L,Vieira SF,Mamanova L,Huang N,Perrone F,Goh Kai'En I,Lisgo SN,Katan M,Leonard S,Oliver TRW,Hook CE,Nayak K,Campos LS,Domínguez Conde C,Stephenson E,Engelbert J,Botting RA,Polanski K,van Dongen S,Patel M,Morgan MD,Marioni JC,Bayraktar OA,Meyer KB,He X,Barker RA,Uhlig HH,Mahbubani KT,Saeb-Parsy K,Zilbauer M,Clatworthy MR,Haniffa M,James KR,Teichmann SA

Published figure using CD32b monoclonal antibody (Product # 17-0321-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Signaling through the Inhibitory Fc Receptor FcγRIIB Induces CD8⁺ T Cell Apoptosis to Limit T Cell Immunity.

Immunity

Morris AB,Farley CR,Pinelli DF,Adams LE,Cragg MS,Boss JM,Scharer CD,Fribourg M,Cravedi P,Heeger PS,Ford ML

17-0321-82 was used in Flow Cytometry to demonstrate a cell-intrinsic coinhibitory function of FcγRIIB in regulating CD8+ T cell immunity.

Tue Jan 14 00:00:00 EST 2020

Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity.

Nature communications

Qi X,Li F,Wu Y,Cheng C,Han P,Wang J,Yang X

17-0321-82 was used in Flow Cytometry to develop LVGN6051, a humanized 4-1BB mAb-AG that shows high anti-tumor efficacy in the absence of liver toxicity in a mouse model of cancer immunotherapy.

Mon May 20 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD32; CD32 receptor 2; CD32 receptor 2a; CD32 receptor 2b; CD32A; Fc gamma receptor IIB; Fc gamma RIIB; Fc-gamma RII; fc-gamma RII-a; Fc-gamma-RII; fc-gamma-RIIa; fc-gamma-RIIB; FcgammaRIIB; FcGR; FCGR2A1; fcRII-a; FGFR2B; IgG Fc receptor II beta; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; Lymphocyte antigen 17

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Gene Aliases: AI528646; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD32b Monoclonal Antibody (AT130-2), APC, eBioscience™

CD32b Antibody (17-0321-82) in Flow

Product Details

17-0321-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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