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Invitrogen
CD335 (NKp46) Monoclonal Antibody (9E2), Functional Grade, eBioscience™
FIGURE: 1 / 2
CD335 (NKp46) Antibody (16-3359-82) in Flow
Normal human peripheral blood cells cells were stained with CD56 Monoclonal Antibody, APC (Product # 17-0566-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (left) or 0.125 µg of CD335 Monoclonal Antibody, Functional Grade (right) followed by Anti-IgG1 Monoclonal Antibody, Biotin (Product # 13-4015-82) and Streptavidin, PE (Product #12-4317-87). Cells in the lymphocyte gate were used for analysis.
Product Details
16-3359-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
9E2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573084
Product Specific Information
Description: This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46. Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44. This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells. CD335 also interacts with pancreatic beta cells during the development of type I diabetes.
The 9E2 antibody has been reported to induce cytotoxicity.
Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis and to induce cytotoxicity in functional assays.
Applications Tested: This 9E2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1-Mar; CD335; hNKp46; Ly-94; Lymphocyte antigen 94 homolog; lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK cell-activating receptor; NK-p46
Gene Aliases: CD335; LY94; NCR1; NK-p46; NKP46
UniProt ID: (Human) O76036
Entrez Gene ID: (Human) 9437
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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