Invitrogen

CD337 (NKp30) Monoclonal Antibody (AF29-4D12), PE, eBioscience™

1 Published Figure

3 References

View all (14) NKp30 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD337 (NKp30) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD337 (NKp30) Antibody (12-3379-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD337 (NKp30) PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-3379-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AF29-4D12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572609

Product Specific Information

Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30. Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells. Several ligands for this receptor have been reported, including B7-H6.

The AF29-4D12 antibody has been reported to crossreact to rhesus macaque.

Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF29-4D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients.

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

12-3379-42 was used in Flow Cytometry to suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.

Mon Jun 01 00:00:00 EDT 2020

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

12-3379-42 was used in Flow Cytometry to suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.

Mon Jun 01 00:00:00 EDT 2020

Identification of Human Memory-Like NK Cells.

Current protocols in cytometry

Kovalenko EI,Streltsova MA,Kanevskiy LM,Erokhina SA,Telford WG

12-3379-42 was used in Flow cytometry/Cell sorting to describe an approach for identification and analysis of adaptive NK cells in human peripheral blood. CD57-positive cells with high expression of activating-receptor NKG2C, increased expression of KIR receptors, lack of co-expression with inhibitory receptor NKG2A, and decreased expression of activating receptor NCR3 (NKp30) all characterize this cell type.

Thu Jan 05 00:00:00 EST 2017

Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis.

Nature communications

Trabanelli S,Chevalier MF,Martinez-Usatorre A,Gomez-Cadena A,Salomé B,Lecciso M,Salvestrini V,Verdeil G,Racle J,Papayannidis C,Morita H,Pizzitola I,Grandclément C,Bohner P,Bruni E,Girotra M,Pallavi R,Falvo P,Leibundgut EO,Baerlocher GM,Carlo-Stella C,Taurino D,Santoro A,Spinelli O,Rambaldi A,Giarin E,Basso G,Tresoldi C,Ciceri F,Gfeller D,Akdis CA,Mazzarella L,Minucci S,Pelicci PG,Marcenaro E,McKenzie ANJ,Vanhecke D,Coukos G,Mavilio D,Curti A,Derré L,Jandus C

12-3379 was used in Flow cytometry/Cell sorting to elucidate the function of group 2 innate lymphoid cells in human cancer.

Tue Sep 19 00:00:00 EDT 2017

Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis.

Nature communications

12-3379 was used in Flow cytometry/Cell sorting to elucidate the function of group 2 innate lymphoid cells in human cancer.

Tue Sep 19 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Activating natural killer receptor p30; activating NK-A1 receptor; CD337; lymphocyte antigen 117; Natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; natural killer cell receptor; NK-p30

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Gene Aliases: 1C7; CD337; LY117; MALS; NCR3; NKp30

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UniProt ID: (Human) O14931, (Rhesus monkey) Q5TM24

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Entrez Gene ID: (Human) 259197, (Rhesus monkey) 715574

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Function(s)

receptor activity receptor binding protein homodimerization activity cell adhesion molecule binding

Process(es)

positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target inflammatory response immune response homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition susceptibility to natural killer cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity regulation of immune response susceptibility to T cell mediated cytotoxicity

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CD337 (NKp30) Monoclonal Antibody (AF29-4D12), PE, eBioscience™

CD337 (NKp30) Antibody (12-3379-42) in Flow

Product Details

12-3379-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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