Invitrogen

CD34 Recombinant Rabbit Monoclonal Antibody (SI16-01)

1 Published Figure

3 References

View all (163) CD34 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (1)

CD34 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

CD34 Antibody (MA5-32059) in ICC/IF

Immunocytochemical analysis of CD34 in SH-SY-5Y cells using a CD34 Monoclonal antibody (Product # MA5-32059) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD34 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-32059

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:10,000

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SI16-01

Immunogen

Synthetic peptide within Human CD34 aa 336-385

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809353

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Estradiol enhances T-type calcium channel activation in human myometrium telocytes.

The Journal of reproduction and development

Zhu L,Shangguan T,Chen P,Wang Y,Xiao L,Liu H,He W

MA5-32059 was used in Immunocytochemistry, Immunohistochemistry, Western Blot to demonstrate that telocytes exist in the human myometrium, expressing T-type calcium channels and estradiol-enhanced T-type calcium currents, which may be a reasonable explanation for the origin of uterine peristalsis.

Mon Apr 03 00:00:00 EDT 2023

Distinct bulge stem cell populations maintain the pilosebaceous unit in a β-catenin-dependent manner.

iScience

Han J,Lin K,Choo H,Chen Y,Zhang X,Xu RH,Wang X,Wu Y

MA5-32059 was used in Immunohistochemistry to ndicated that the upper bulge stem cells contributed extensively to the SG replenishment during hair cycling, while HFSCs in the lower bugle did not and indicate that HFSCs contributed to SG replenishment in a β-catenin-dependent manner.

Fri Jan 20 00:00:00 EST 2023

β-Catenin Signaling Evokes Hair Follicle Senescence by Accelerating the Differentiation of Hair Follicle Mesenchymal Progenitors.

Frontiers in cell and developmental biology

Han J,Lin K,Choo H,He J,Wang X,Wu Y,Chen X

MA5-32059 was used in Immunohistochemistry to reveal that the regulation of β-catenin signaling in hfDSCs may potentially become an important subject for future exploration in development of clinically effective therapies for hair loss treatment and an excellent model for revealing new therapeutic approaches to reverse aging or retarding the development of alopecia.

Sat Jul 16 00:00:00 EDT 2022

β-Catenin Signaling Evokes Hair Follicle Senescence by Accelerating the Differentiation of Hair Follicle Mesenchymal Progenitors.

Frontiers in cell and developmental biology

Han J,Lin K,Choo H,He J,Wang X,Wu Y,Chen X

Sat Jul 16 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: CD34; CD34 antigen; cluster designation 34; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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Gene Aliases: AU040960; CD34

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UniProt ID: (Human) P28906, (Mouse) Q64314

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Entrez Gene ID: (Human) 947, (Rat) 305081, (Mouse) 12490

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis cell adhesion

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CD34 Recombinant Rabbit Monoclonal Antibody (SI16-01)

CD34 Antibody (MA5-32059) in ICC/IF

Product Details

MA5-32059

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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