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CD34 (soluble) Antibody (500-P327BT-25UG) in WB
Western Blot: To detect Human sCD34 by Western Blot analysis, CD34 (soluble) Polyclonal Antibody, Biotin (Product # 500-P327BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sCD34 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P327BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 27.4 kDa Recombinant Human sCD34
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930438
Product Specific Information
AA Sequence of recombinant protein: LDNNGTATPE LPTQGTFSNV STNVSYQETT TPSTLGSTSL HPVSQHGNEA TTNITETTVK FTSTSVITSV YGNTNSSVQS QTSVISTVFT TPANVSTPET TLKPSLSPGN VSDLSTTSTS LATSPTKPYT SSSPILSDIK AEIKCSGIRE VKLTQGICLE QNKTSSCAEF KKDRGEGLAR VLCGEEQADA DAGAQVCSLL LAQSEVRPQC LLLVLANRTE ISSKLQLMKK HQSDLKKLGI LDFTEQDVAS HQSYSQKT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD34. Anti-Human sCD34-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sCD34 by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD34 (500-P327) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sCD34.
Western Blot: To detect Human sCD34 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sCD34 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P327BT-1MG will be provided as 2 x 500 µg
Target Information
CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2
Gene Aliases: CD34
UniProt ID: (Human) P28906
Entrez Gene ID: (Human) 947
tissue homeostasis
endothelial cell proliferation
glomerular filtration
endothelium development
cell-matrix adhesion
signal transduction
regulation of blood pressure
cell proliferation
positive regulation of gene expression
negative regulation of gene expression
single organismal cell-cell adhesion
hemopoiesis
negative regulation of blood coagulation
negative regulation of tumor necrosis factor production
positive regulation of interleukin-10 production
mesangial cell-matrix adhesion
paracrine signaling
positive regulation of odontogenesis
negative regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
cell motility
regulation of immune response
leukocyte migration
transdifferentiation
vascular wound healing
hematopoietic stem cell proliferation
positive regulation of transforming growth factor beta production
positive regulation of granulocyte colony-stimulating factor production
extracellular exosome assembly
glomerular endothelium development
stem cell proliferation
metanephric glomerular mesangial cell differentiation
negative regulation of cellular response to heat
negative regulation of cellular response to hypoxia
negative regulation of interleukin-2 secretion
positive regulation of glial cell-derived neurotrophic factor secretion
negative regulation of neuron death
positive regulation of vasculogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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