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CD35 Antibody (MA5-44022) in Flow
Flow Cytometry analysis of CD35 using CD35 Monoclonal Antibody (E11) (Product # MA5-44022). Separation of human CD35 positive lymphocytes (red-filled) from CD35 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 μg/mL, GAM APC).
Product Details
MA5-44022
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
E11
Immunogen
Acute monocytic leukemia cells and normal blood monocytes
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912954
Product Specific Information
This antibody recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, follicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
Immunohistochemistry (Paraffin): Heat mediated antigen retrieval; Immunohistochemistry (Frozen): acetone fixation.
Target Information
CD35 (CR1, Complement Receptor 1) is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. CD35 protein mediates cellular binding to particles and immune complexes that have activated complement. The CD35 complex is four different allotypes (C,A,B,D) C is 160 kDa; A is 190 kDa; B is 220 kDa and D is 250 kDa. CD35 is involved in the processing of immune complexes, promoting of binding and phagocytosis of C3b complexes and inhibition of complement activation. CD35 is on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukemias, follicular dendritic reticulum cells and is negative on basophils. Decreases in expression of CD35 protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in the CD35 gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants encoding different isoforms of CD35 have been characterized. An additional secreted form of CD35 have also been described but has not been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C3 binding protein; C3-binding protein; C3b/C4b receptor; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); Complement Component (3b/4b) receptor 1 including Knops blood group system; Complement receptor type 1; Knops blood group antigen
Gene Aliases: C3BR; C4BR; CD35; CR1; KN
UniProt ID: (Human) P17927
Entrez Gene ID: (Human) 1378
complement receptor mediated signaling pathway
complement activation, classical pathway
regulation of complement activation
innate immune response
negative regulation of complement activation, alternative pathway
negative regulation of complement activation, classical pathway
viral entry into host cell
negative regulation of serine-type endopeptidase activity
positive regulation of serine-type endopeptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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