Invitrogen

CD357 (AITR/GITR) Monoclonal Antibody (eBioAITR), PE, eBioscience™

CD357 (AITR/GITR) Antibody in Flow Cytometry (Flow)

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CD357 (AITR/GITR) Antibody (12-5875-42) in Flow

Staining of 3-day Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0039-81 and Product # 16-0289-81)-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD357 (AITR/GITR) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used... View More

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Product Details

12-5875-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioAITR

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572634

Product Specific Information

Description: The monoclonal antibody reacts with human AITR, Activation Inducible TNFR family member, an approximately 25 kDa member of the TNFR superfamily. AITR mRNA is detected in lymph node, peripheral blood leukocytes and weakly in spleen. AITR mRNA expression was upregulated within 24 hour on PMA/ionomycin or PHA stimulated PBMC. At the protein level, AITR is expressed by a small population of activated PBMC. AITR associates with TRAF1, TRAF2 and TRAF3 and induces nuclear factor NF-kappaB activation via TRAF2. Recently TL6 (AITRL) has been reported as the ligand for AITR. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.

Applications Reported: This eBioAITR antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioAITR antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Optimal target saturation of ligand-blocking anti-GITR antibody IBI37G5 dictates FcγR-independent GITR agonism and antitumor activity.

Cell reports. Medicine

Liu H,Wu W,Sun G,Chia T,Cao L,Liu X,Guan J,Fu F,Yao Y,Wu Z,Zhou S,Wang J,Lu J,Kuang Z,Wu M,He L,Shao Z,Wu D,Chen B,Xu W,Wang Z,He K

12-5875-42 was used in Flow cytometry/Cell sorting to propose an alternative dose-finding strategy that does not rely on the traditional maximal tolerated dose (MTD)-based paradigm but instead on utilizing the RO-function relations as biomarker to guide the clinical translation of GITR and similar co-stimulatory agonists.

Tue Jun 21 00:00:00 EDT 2022

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

12587542 was used in flow cytometry to identify and characterize CXCR5hiCD44hiCD8 T cells in HIV-infected individuals

Sat Oct 01 00:00:00 EDT 2016

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

12587542 was used in flow cytometry to identify and characterize CXCR5hiCD44hiCD8 T cells in HIV-infected individuals

Sat Oct 01 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

12-5875 was used in Flow cytometry/Cell sorting to determine TFR contribute to inefficient germinal centre responses and inhibit HIV and SIV clearance.

Tue Oct 20 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

12-5875 was used in Flow cytometry/Cell sorting to determine TFR contribute to inefficient germinal centre responses and inhibit HIV and SIV clearance.

Tue Oct 20 00:00:00 EDT 2015

Highly heterogeneous, activated, and short-lived regulatory T cells during chronic filarial infection.

European journal of immunology

Metenou S,Coulibaly YI,Sturdevant D,Dolo H,Diallo AA,Soumaoro L,Coulibaly ME,Kanakabandi K,Porcella SF,Klion AD,Nutman TB

12-5875 was used in Flow cytometry/Cell sorting to study how in filarial infection, the expanded Treg-cell populations are heterogeneous, short-lived, activated, and express higher levels of molecules known to modulate immune responsiveness.

Tue Jul 01 00:00:00 EDT 2014

Elevated levels of CD4(+)CD25(+)FoxP3(+) T cells in systemic sclerosis patients contribute to the secretion of IL-17 and immunosuppression dysfunction.

PloS one

Liu X,Gao N,Li M,Xu D,Hou Y,Wang Q,Zhang G,Sun Q,Zhang H,Zeng X

12-5875 was used in Flow cytometry/Cell sorting to investigate the role of Treg subsets in the immune imbalance in naive systemic sclerosis (SSc), showing that elevated levels of CD4(+)CD25(+)FoxP3(+) T cells contribute to the secretion of IL-17 and immunosuppression dysfunction.

Thu Jan 16 00:00:00 EST 2014

Transfer of regulatory properties from tolerogenic to proinflammatory dendritic cells via induced autoreactive regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kleijwegt FS,Laban S,Duinkerken G,Joosten AM,Koeleman BP,Nikolic T,Roep BO

12-5875 was used in Flow cytometry/Cell sorting to study the mechanism by which infectious tolerance applies to human dendritic cells.

Thu Dec 15 00:00:00 EST 2011

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Sun Aug 15 00:00:00 EDT 2010

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Sun Aug 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Activation-inducible TNFR family receptor; CD357; Glucocorticoid-induced TNFR-related protein; PRO364; TNF receptor superfamily activation-inducible protein; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily, member 18; UNQ319

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Gene Aliases: AITR; CD357; GITR; GITR-D; TNFRSF18; UNQ319/PRO364

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UniProt ID: (Human) Q9Y5U5

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Entrez Gene ID: (Human) 8784

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Function(s)

tumor necrosis factor-activated receptor activity transmembrane signal receptor

Process(es)

positive regulation of leukocyte migration inflammatory response immune response signal transduction multicellular organism development extrinsic apoptotic signaling pathway via death domain receptors response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat1 protein negative regulation of apoptotic process positive regulation of cell adhesion

Performance Guarantee

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CD357 (AITR/GITR) Monoclonal Antibody (eBioAITR), PE, eBioscience™

CD357 (AITR/GITR) Antibody (12-5875-42) in Flow

Product Details

12-5875-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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