Invitrogen

CD36 Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

4 References

View all (91) CD36 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD36 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

CD36 Antibody (PA5-27236) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CD36 (Product # PA5-27236) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

CD36 Antibody in Immunohistochemistry (IHC)

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CD36 Antibody — The CD14 and CD36 co-receptors amplify TLR2-mediated cell activation induced by microneme proteins. HEK293T cells transfected with (A,B) TLR2/1 or (C,D) TLR2/6 were co-transfected with CD14 and CD36 either together or alone, along with an NF-κB-dependent luciferase reporter construct (pELAM-firefly luciferase) and a Renilla luciferase reporter construct (an internal control). The total amount of DNA in each transfection was kept constant by adding empty vector. After 48 h of transfection, the cells were stimulated with rMIC1 (50 nM) or rMIC4 (50 nM). Pam3CSK4 (P3C, 1 nM) was used as a positive control for TLR2/1, and FSL-1 (1 nM) was used as a positive control for TLR2/6. Medium was used as the negative control. Twenty-four hours post-stimulation, the IL-8 concentration in cell supernatants were assessed by ELISA. Statistical analysis was performed to compare (*) the responses in cells lacking one or more co-receptor to the responses when both CD14 and CD36 were expressed. The responses in cells expressing one co-receptor, both co-receptors, or no co-receptor (#) were compared after stimulation with microneme protein versus the responses in the negative control cells (medium). The data are from three independent experiments yielding similar results (E) BMDMs obtained from wild type C57BL/6 mice were transduced with lentivirus vectors encoding shRNA sequences for CD14 and CD36 or a non-target control shRNA. The expression levels of CD14 and CD36 were evaluated by immunoblottin {KD}

Product Details

PA5-27236

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 302 and 395 of Human CD36

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544712

Product Specific Information

Recommended positive controls: K562, mouse brown adipose, RAW264.7.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion of LDLRAP1 Induces Atherosclerotic Plaque Formation, Insulin Resistance, and Dysregulated Insulin Response in Adipose Tissue.

The American journal of pathology

Leigh T,Kawai T,Preston K,Kelemen S,Okune R,St Paul A,Corbett C,Peluzzo AM,Yu J,Scalia RG,Autieri MV

PA5-27236 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that LDLRAP1 is highly expressed in visceral adipose tissue, and LDLRAP1-/- adipocytes are significantly larger, have reduced glucose uptake and AKT phosphorylation, but have increased CD36 expression.

Fri Jul 01 00:00:00 EDT 2022

Tart Cherry Juice and Seeds Affect Pro-Inflammatory Markers in Visceral Adipose Tissue of High-Fat Diet Obese Rats.

Molecules (Basel, Switzerland)

Moruzzi M,Klöting N,Blüher M,Martinelli I,Tayebati SK,Gabrielli MG,Roy P,Micioni Di Bonaventura MV,Cifani C,Lupidi G,Amenta F,Tomassoni D

PA5-27236 was used in Immunohistochemistry to investigate the potential positive effects of tart cherries on rats with Diet-Induced Obesity (DIO).

Fri Mar 05 00:00:00 EST 2021

Tart Cherry Juice and Seeds Affect Pro-Inflammatory Markers in Visceral Adipose Tissue of High-Fat Diet Obese Rats.

Molecules (Basel, Switzerland)

Moruzzi M,Klöting N,Blüher M,Martinelli I,Tayebati SK,Gabrielli MG,Roy P,Micioni Di Bonaventura MV,Cifani C,Lupidi G,Amenta F,Tomassoni D

PA5-27236 was used in Immunohistochemistry to investigate the potential positive effects of tart cherries on rats with Diet-Induced Obesity (DIO).

Fri Mar 05 00:00:00 EST 2021

Receptor Heterodimerization and Co-Receptor Engagement in TLR2 Activation Induced by MIC1 and MIC4 from Toxoplasma gondii.

International journal of molecular sciences

Costa Mendonça-Natividade F,Duque Lopes C,Ricci-Azevedo R,Sardinha-Silva A,Figueiredo Pinzan C,Paiva Alegre-Maller AC,L Nohara L,B Carneiro A,Panunto-Castelo A,C Almeida I,Roque-Barreira MC

Published figure using CD36 polyclonal antibody (Product # PA5-27236) in Western Blot

Thu Oct 10 00:00:00 EDT 2019

Osteogenic Potential of Caspases Related to Endochondral Ossification.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Janečková E,Bíliková P,Matalová E

PA5-27236 was used in Immunohistochemistry to show the distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis.

Mon Jan 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor

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Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3

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UniProt ID: (Human) P16671, (Mouse) Q08857

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Entrez Gene ID: (Human) 948, (Mouse) 12491

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Function(s)

low-density lipoprotein receptor activity protein binding high-density lipoprotein particle binding lipid binding low-density lipoprotein particle binding transforming growth factor beta binding thrombospondin receptor activity lipoteichoic acid receptor activity lipoprotein particle binding oleic acid binding membrane trafficking regulatory protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD36 Polyclonal Antibody

CD36 Antibody (PA5-27236) in IHC (P)

Product Details

PA5-27236

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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