Invitrogen

CD36 Monoclonal Antibody (185-1G2)

CD36 Antibody in Immunocytochemistry (ICC/IF)

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CD36 Antibody (MA5-14112) in ICC/IF

Immunofluorescent analysis of CD36 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD36 monoclonal antibody (Product # MA5-14112) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More

CD36 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

MA5-14112

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 6 publications 6 publications

Neutralization (Neu)

View 3 publications 3 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

185-1G2

Immunogen

Stimulated human leukocytes

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11007635

Product Specific Information

MA5-14112 targets CD36 in FACS, immunofluorescence, and immunohistochemistry (frozen) applications and shows reactivity with human and mouse samples.

The MA5-14112 immunogen is stimulated human leukocytes.

Target Information

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration.

Nature

Moiseeva V,Cisneros A,Sica V,Deryagin O,Lai Y,Jung S,Andrés E,An J,Segalés J,Ortet L,Lukesova V,Volpe G,Benguria A,Dopazo A,Benitah SA,Urano Y,Del Sol A,Esteban MA,Ohkawa Y,Serrano AL,Perdiguero E,Muñoz-Cánoves P

MA5-14112 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to provide a technique for isolating in vivo senescent cells, define a senescence blueprint for muscle, and uncover unproductive functional interactions between senescent cells and stem cells in regenerative niches that can be overcome.

Sun Jan 01 00:00:00 EST 2023

Expanded IL-22⁺ Group 3 Innate Lymphoid Cells and Role of Oxidized LDL-C in the Pathogenesis of Axial Spondyloarthritis with Dyslipidaemia.

Immune network

Min HK,Moon J,Lee SY,Lee AR,Lee CR,Lee J,Kwok SK,Cho ML,Park SH

MA5-14112 was used in Inhibition Assays to explore the role of circulating group 3 innate lymphoid cells in axial spondyloarthritis and the impact of dyslipidaemia on its pathogenesis.

Wed Dec 01 00:00:00 EST 2021

Expanded IL-22⁺ Group 3 Innate Lymphoid Cells and Role of Oxidized LDL-C in the Pathogenesis of Axial Spondyloarthritis with Dyslipidaemia.

Immune network

Min HK,Moon J,Lee SY,Lee AR,Lee CR,Lee J,Kwok SK,Cho ML,Park SH

MA5-14112 was used in Inhibition Assays to explore the role of circulating group 3 innate lymphoid cells in axial spondyloarthritis and the impact of dyslipidaemia on its pathogenesis.

Wed Dec 01 00:00:00 EST 2021

Empagliflozin Ameliorates Free Fatty Acid Induced-Lipotoxicity in Renal Proximal Tubular Cells via the PPARγ/CD36 Pathway in Obese Mice.

International journal of molecular sciences

Huang CC,Chou CA,Chen WY,Yang JL,Lee WC,Chen JB,Lee CT,Li LC

Published figure using CD36 monoclonal antibody (Product # MA5-14112) in Western Blot

Wed Nov 17 00:00:00 EST 2021

Empagliflozin Ameliorates Free Fatty Acid Induced-Lipotoxicity in Renal Proximal Tubular Cells via the PPARγ/CD36 Pathway in Obese Mice.

International journal of molecular sciences

Huang CC,Chou CA,Chen WY,Yang JL,Lee WC,Chen JB,Lee CT,Li LC

Published figure using CD36 monoclonal antibody (Product # MA5-14112) in Western Blot

Wed Nov 17 00:00:00 EST 2021

Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages.

PloS one

Kitaura A,Nishinaka T,Hamasaki S,Hatipoglu OF,Wake H,Nishibori M,Mori S,Nakao S,Takahashi H

MA5-14112 was used in Flow cytometry/Cell sorting to indicate that the receptor for the AGE-initiated pathway partially impairs the immune response in diabetes patients.

Thu Jun 24 00:00:00 EDT 2021

SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.

Nature

Huang L,Chambliss KL,Gao X,Yuhanna IS,Behling-Kelly E,Bergaya S,Ahmed M,Michaely P,Luby-Phelps K,Darehshouri A,Xu L,Fisher EA,Ge WP,Mineo C,Shaul PW

Published figure using CD36 monoclonal antibody (Product # MA5-14112) in Western Blot

Wed May 01 00:00:00 EDT 2019

Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis.

Scientific reports

Hamasaki S,Kobori T,Yamazaki Y,Kitaura A,Niwa A,Nishinaka T,Nishibori M,Mori S,Nakao S,Takahashi H

Published figure using CD36 monoclonal antibody (Product # MA5-14112) in Neutralization

Thu Apr 12 00:00:00 EDT 2018

EphrinA1-Fc attenuates myocardial ischemia/reperfusion injury in mice.

PloS one

DuSablon A,Parks J,Whitehurst K,Estes H,Chase R,Vlahos E,Sharma U,Wert D,Virag J

MA5-14112 was used in Western Blotting to suggest that ephrinA1-Fc enhances the capacity of the heart to withstand an ischemic insult.

Tue Jan 16 00:00:00 EST 2018

Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.

Journal for immunotherapy of cancer

Pardee AD,Yano H,Weinstein AM,Ponce AA,Ethridge AD,Normolle DP,Vujanovic L,Mizejewski GJ,Watkins SC,Butterfield LH

MA5-14112 was used in flow cytometry to evaluate the tumor-associated antigen alpha-fetoprotein as a target for immunotherapy

Wed Jul 22 00:00:00 EDT 2015

Histopathologies, immunolocalization, and a glycan binding screen provide insights into Plasmodium falciparum interactions with the human placenta.

Biology of reproduction

Hromatka BS,Ngeleza S,Adibi JJ,Niles RK,Tshefu AK,Fisher SJ

MA5-14112 was used in immunohistochemistry to study the mechanisms underlying the interaction of Plasmodium falciparum with the human placenta

Sat Jun 01 00:00:00 EDT 2013

Implication of scavenger receptors in the interactions between diesel exhaust particles and immature or mature dendritic cells.

Particle and fibre toxicology

Taront S,Dieudonné A,Blanchard S,Jeannin P,Lassalle P,Delneste Y,Gosset P

MA5-14112 was used in flow cytometry to study the involvement of scavenger receptors in toxicopathology of diesel exhaust particles

Fri Mar 13 00:00:00 EDT 2009

The cysteine-rich interdomain region from the highly variable plasmodium falciparum erythrocyte membrane protein-1 exhibits a conserved structure.

PLoS pathogens

Klein MM,Gittis AG,Su HP,Makobongo MO,Moore JM,Singh S,Miller LH,Garboczi DN

MA5-14112 was used in flow cytometry to study the conserved structure of the cysteine-rich interdomain region from the highly variable Plasmodium falciparum erythrocyte membrane protein-1

Fri Sep 05 00:00:00 EDT 2008

Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.

The Journal of pathology

Martens JH,Kzhyshkowska J,Falkowski-Hansen M,Schledzewski K,Gratchev A,Mansmann U,Schmuttermaier C,Dippel E,Koenen W,Riedel F,Sankala M,Tryggvason K,Kobzik L,Moldenhauer G,Arnold B,Goerdt S

MA5-14112 was used in immunohistochemistry To study the molecular mechanism for the arrest of metastatic tumour cells in human lymph node sinuses

Wed Mar 01 00:00:00 EST 2006

CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1.

The Journal of biological chemistry

Isenberg JS,Ridnour LA,Dimitry J,Frazier WA,Wink DA,Roberts DD

MA5-14112 was used in Immunohistochemistry to show ligation of either CD36 or CD47 is sufficient to inhibit NO-stimulated vascular cell responses and cGMP signaling, but only CD47 is necessary for this activity of thrombospondin-1 at physiological concentrations.

Fri Sep 08 00:00:00 EDT 2006

Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by beta1 integrins.

The Journal of cell biology

Short SM,Derrien A,Narsimhan RP,Lawler J,Ingber DE,Zetter BR

MA5-14112 was used in blocking or activating experiment to investigate the mechanism for anti-angiogenic effect of thrombospondin-1

Mon Feb 14 00:00:00 EST 2005

Phosphatidylserine regulates the maturation of human dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Chen X,Doffek K,Sugg SL,Shilyansky J

MA5-14112 was used in blocking or activating experiment to study the role of phosphatidylserine in regulating human dendritic cell maturation

Wed Sep 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor

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Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3

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UniProt ID: (Human) P16671, (Mouse) Q08857

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Entrez Gene ID: (Human) 948, (Mouse) 12491

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Function(s)

low-density lipoprotein receptor activity protein binding high-density lipoprotein particle binding lipid binding low-density lipoprotein particle binding transforming growth factor beta binding thrombospondin receptor activity lipoteichoic acid receptor activity lipoprotein particle binding oleic acid binding membrane trafficking regulatory protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD36 Monoclonal Antibody (185-1G2)

CD36 Antibody (MA5-14112) in ICC/IF

Product Details

MA5-14112

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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