Invitrogen

CD36 Monoclonal Antibody (eBioNL07 (NL07)), FITC, eBioscience™

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Antibody Testing Data (1)
Published Figures (7)

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CD36 Antibody (11-0369-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgM Isotype Control FITC (Product # 11-4752-80) (blue histogram) or Anti-Human CD36 FITC (purple histogram). Cells in the monocyte gate were used for analysis.

CD36 Antibody in Immunohistochemistry (IHC)

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Product Details

11-0369-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Recommended Isotype Control

Mouse IgM Isotype Control (11E10), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioNL07 (NL07)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718972

Product Specific Information

Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.

Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

11-0369 was used in Flow cytometry/Cell sorting to determine the basis for aberrant megakaryopoiesis in myelofibrosis using single-cell-omics.

Thu May 07 00:00:00 EDT 2020

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

11-0369 was used in Flow cytometry/Cell sorting to determine the basis for aberrant megakaryopoiesis in myelofibrosis using single-cell-omics.

Thu May 07 00:00:00 EDT 2020

Genome-Wide Transcriptional Regulation of the Long Non-coding RNA Steroid Receptor RNA Activator in Human Erythroblasts.

Frontiers in genetics

Sawaengdee W,Cui K,Zhao K,Hongeng S,Fucharoen S,Wongtrakoongate P

Published figure using CD36 monoclonal antibody (Product # 11-0369-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD36 monoclonal antibody (Product # 11-0369-42) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD36 monoclonal antibody (Product # 11-0369-42) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2018

Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.

Immunology

Swindle EJ,Brown JM,Rådinger M,DeLeo FR,Metcalfe DD

11-0369 was used in Flow cytometry/Cell sorting to investigate the effect of interferon-γ on the biological responses of human mast cells to Staphylococcus aureus.

Sun Nov 01 00:00:00 EDT 2015

Developmental transcriptome analysis of human erythropoiesis.

Human molecular genetics

Shi L,Lin YH,Sierant MC,Zhu F,Cui S,Guan Y,Sartor MA,Tanabe O,Lim KC,Engel JD

11-0369 was used in Flow cytometry/Cell sorting to perform RNA-seq on CD34(+) cells after ex vivo differentiation from the earliest into the most mature erythroid cell stages.

Mon Sep 01 00:00:00 EDT 2014

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

11-0369 was used in Flow cytometry/Cell sorting to investigate the potential of nuclear reprogramming as a broadly applicable therapeutic strategy for the treatment of cancer.

Thu May 02 00:00:00 EDT 2013

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

11-0369 was used in Flow cytometry/Cell sorting to investigate the potential of nuclear reprogramming as a broadly applicable therapeutic strategy for the treatment of cancer.

Thu May 02 00:00:00 EDT 2013

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

11-0369 was used in Flow cytometry/Cell sorting to investigate the potential of nuclear reprogramming as a broadly applicable therapeutic strategy for the treatment of cancer.

Thu May 02 00:00:00 EDT 2013

IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis.

Scientific reports

Xia F,Li R,Wang C,Yang S,Tian L,Dong H,Pei C,He S,Jiang P,Cheng H,Fang S,Li H,Xu H

Published figure using CD36 monoclonal antibody (Product # 11-0369-42) in Flow Cytometry

Thu Oct 24 00:00:00 EDT 2013

An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.

The Journal of clinical investigation

Sindrilaru A,Peters T,Wieschalka S,Baican C,Baican A,Peter H,Hainzl A,Schatz S,Qi Y,Schlecht A,Weiss JM,Wlaschek M,Sunderkötter C,Scharffetter-Kochanek K

11-0369 was used in Flow cytometry/Cell sorting to study human subjects with chronic venous leg ulcers and devise a human and mouse disease model for macrophage-driven chronic inflammation.

Tue Mar 01 00:00:00 EST 2011

CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Landrigan A,Wong MT,Utz PJ

11-0369 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells.

Thu Sep 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor

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Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3

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UniProt ID: (Human) P16671

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Entrez Gene ID: (Human) 948

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Function(s)

low-density lipoprotein receptor activity protein binding high-density lipoprotein particle binding lipid binding low-density lipoprotein particle binding transforming growth factor beta binding thrombospondin receptor activity lipoteichoic acid receptor activity lipoprotein particle binding membrane trafficking regulatory protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD36 Monoclonal Antibody (eBioNL07 (NL07)), FITC, eBioscience™

CD36 Antibody (11-0369-42) in Flow

Product Details

11-0369-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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