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Invitrogen
CD36 Monoclonal Antibody (eBioNL07 (NL07)), eFluor™ 660, eBioscience™
Product Details
50-0369-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgM
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioNL07 (NL07)
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10596358
Product Specific Information
Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.
Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis of normal human perioheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor
Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3
UniProt ID: (Human) P16671
Entrez Gene ID: (Human) 948
low-density lipoprotein receptor activity
protein binding
high-density lipoprotein particle binding
lipid binding
low-density lipoprotein particle binding
transforming growth factor beta binding
thrombospondin receptor activity
lipoteichoic acid receptor activity
lipoprotein particle binding
membrane trafficking regulatory protein
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cell-matrix adhesion
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
platelet degranulation
MyD88-dependent toll-like receptor signaling pathway
lipid metabolic process
receptor-mediated endocytosis
phagocytosis, recognition
immune response
cell adhesion
cell surface receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
nitric oxide mediated signal transduction
blood coagulation
positive regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol storage
lipid storage
cGMP-mediated signaling
positive regulation of blood coagulation
intestinal cholesterol absorption
cholesterol transport
receptor internalization
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
response to lipid
triglyceride transport
plasma lipoprotein particle clearance
low-density lipoprotein particle clearance
response to stilbenoid
toll-like receptor TLR6:TLR2 signaling pathway
lipoprotein transport
negative regulation of transcription factor import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
apoptotic cell clearance
negative regulation of growth of symbiont in host
long-chain fatty acid import
interleukin-1 beta secretion
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-positive bacterium
intestinal absorption
sensory perception of taste
low-density lipoprotein particle mediated signaling
positive regulation of phagocytosis, engulfment
positive regulation of macrophage cytokine production
positive regulation of ERK1 and ERK2 cascade
cholesterol import
response to fatty acid
response to linoleic acid
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to low-density lipoprotein particle stimulus
cellular response to hydroperoxide
cellular response to diacyl bacterial lipopeptide
positive regulation of NLRP3 inflammasome complex assembly
amyloid fibril formation
regulation of removal of superoxide radicals
positive regulation of blood microparticle formation
positive regulation of reactive oxygen species metabolic process
regulation of energy homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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