Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
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Description: This 2SX21R monoclonal antibody reacts with human CD360 (Interleukin-21 Receptor, IL-21R). IL-21R forms a heterodimeric receptor complex with the common gamma chain to form a functional receptor for IL-21. IL-21 is a class I cytokine of the common gamma chain family of cytokines and signals through the IL-21R complex to activate the JAK/STAT (Janus Kinase/Signal Transducers and Activators of Transcription) signaling pathway. Activation of the JAK/STAT pathway by IL-21 promotes transcription of genes responsive to STAT1 and STAT3. IL-21 is mainly produced by CD4+ T cells and mediates the proliferation, differentiation, and effector functions of cells expressing IL-21R such as B cells, T cells, NK cells, dendritic cells and other populations of myeloid cells. IL-21 and its receptor are implicated in the pathogenesis of chronic viral infections, autoimmunity, allergy, organ transplantation, and cancer.
Applications Reported: This 2SX21R antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2SX21R antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2 (IL2) and interleukin 5 (IL5). This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants encoding the same protein have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Protein Aliases: CD360; IL-21 receptor; Interleukin-21 receptor; MGC10967; Novel interleukin receptor
Gene Aliases: CD360; IL21R; NILR; UNQ3121/PRO10273
UniProt ID: (Human) Q9HBE5
Entrez Gene ID: (Human) 50615
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