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Invitrogen
CD365 (Tim-1) Recombinant Rabbit Monoclonal Antibody (P365D), Alexa Fluor™ 488, eBioscience™
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
CD365 (Tim-1) Antibody (53-3658-42) in Flow
Antibody specificity was demonstrated by siRNA mediated knockdown of CD365. 769P cells were transfected with either non-targeting control siRNA or siRNA against CD365. As expected, loss of signal was observed with CD365 clone P365D in cells transfected with the CD365 specific siRNA (pink histogram) but not the cells transfected with control siRNA (purple histogram). SI denotes the staining index calculated against corresponding isotype controls.
Product Details
53-3658-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
P365D
Immunogen
Protein corresponding to Human CD365 (aa22-aa129)
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Protein A
Storage buffer
proprietary buffer/PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2848432
Product Specific Information
Description: This P365D recombinant rabbit monoclonal antibody reacts with human CD365, which is also known as Tim-1, Kim-1, or Havcr1.
Applications Tested: This P365D antibody has been pre-diluted and tested by flow cytometric analysis of 769P cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail. The endogenous ligand for CD365 is Tim-4. In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus. The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes. Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity. CD365 has also been implicated in the T cell co-stimulation. CD365 is primarily expressed in epithelial cells. It is also highly expressed in A549, 769-P and Caco-2 and cell lines. CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD365; HAVcr-1; Hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM-1; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell immunoglobulin mucin family member 1; T-cell immunoglobulin mucin receptor 1; T-cell membrane protein 1; TIM; TIMD-1
Gene Aliases: CD365; HAVCR; HAVCR-1; HAVCR1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1
UniProt ID: (Human) Q96D42
Entrez Gene ID: (Human) 26762
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