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Invitrogen
CD39 Monoclonal Antibody (5F2), PE, eBioscience™
FIGURE: 1 / 2
CD39 Antibody (12-3390-80) in Flow
Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or 0.25 µg of Anti-Mouse CD39 PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-3390-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5F2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10671014
Product Specific Information
Description: This 5F2 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the predominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, which is another NTPase. Together, these molecules influence inflammatory responses. CD39 is expressed on B cells, Langerhans cells, and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T regs. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.
Applications Reported: This 5F2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5F2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1
Gene Aliases: 2610206B08Rik; AA408691; Cd39; Entpd1; NTPDase-1
UniProt ID: (Mouse) P55772
Entrez Gene ID: (Mouse) 12495
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