Invitrogen

CD3e Monoclonal Antibody (eBio500A2 (500A2)), FITC, eBioscience™

4 Published Figures

10 References

View all (504) CD3 antibodies

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD3e Antibody (11-0033-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 APC (Product # 17-0191-82) and 0.25 µg of Golden Syrian Hamster IgG Isotype Control FITC (Product # 11-4914-81) (left) or 0.25 µg of Anti-Mouse CD3e FITC (right). Cells in the lymphocyte gate were used for analysis.

CD3e Antibody in Immunohistochemistry (IHC)

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Product Details

11-0033-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio500A2 (500A2)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837091

Product Specific Information

Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using CD3e monoclonal antibody (Product # 11-0033-82) in Immunohistochemistry

Tue Nov 23 00:00:00 EST 2021

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

11-0033-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD3e monoclonal antibody (Product # 11-0033-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Activation of the Renin-Angiotensin System Promotes Colitis Development.

Scientific reports

Shi Y,Liu T,He L,Dougherty U,Chen L,Adhikari S,Alpert L,Zhou G,Liu W,Wang J,Deb DK,Hart J,Liu SQ,Kwon J,Pekow J,Rubin DT,Zhao Q,Bissonnette M,Li YC

11-0033 was used in Flow cytometry/Cell sorting to demonstrate that the renin-angiotensin II cascade promotes colitis by stimulating intestinal epithelial cell apoptosis and mucosal TH17 responses in a blood pressure-independent manner.

Wed Jun 08 00:00:00 EDT 2016

Leucocyte expression of complement C5a receptors exacerbates infarct size after myocardial reperfusion injury.

Cardiovascular research

De Hoog VC,Timmers L,Van Duijvenvoorde A,De Jager SC,Van Middelaar BJ,Smeets MB,Woodruff TM,Doevendans PA,Pasterkamp G,Hack CE,De Kleijn DP

11-0033 was used in Flow cytometry/Cell sorting to demonstrate that selective blocking of C5aR might be a promising strategy to prevent myocardial ischaemia-reperfusion injury.

Mon Sep 01 00:00:00 EDT 2014

Immune enhancement activities of silk lutein extract from Bombyx mori cocoons.

Biological research

Promphet P,Bunarsa S,Sutheerawattananonda M,Kunthalert D

11-0033 was used in Flow cytometry/Cell sorting to study how silk lutein extract from Bombyx mori cocoons enhanced both innate and adaptive immune functions.

Mon Apr 28 00:00:00 EDT 2014

Reciprocal effects of rab7 deletion in activated and neglected T cells.

Autophagy

Roy SG,Stevens MW,So L,Edinger AL

11-0033 was used in Flow cytometry/Cell sorting to understand the function of autophagosome degradation in survival of activated T cells, by comparison of the effects of blocking autophagy at an early (atg5-/-) or late (rab7-/-) stage on T cell biology in mice.

Mon Jul 01 00:00:00 EDT 2013

Staphylococcus aureus induces type I IFN signaling in dendritic cells via TLR9.

Journal of immunology (Baltimore, Md. : 1950)

Parker D,Prince A

11-0033 was used in Flow cytometry/Cell sorting to illustrate an additional mechanism through which S. aureus exploits innate immune signalling to facilitate infection.

Mon Oct 15 00:00:00 EDT 2012

IL-33 exacerbates acute kidney injury.

Journal of the American Society of Nephrology : JASN

Akcay A,Nguyen Q,He Z,Turkmen K,Won Lee D,Hernando AA,Altmann C,Toker A,Pacic A,Ljubanovic DG,Jani A,Faubel S,Edelstein CL

Published figure using CD3e monoclonal antibody (Product # 11-0033-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2011

Virulence of Toxoplasma gondii is associated with distinct dendritic cell responses and reduced numbers of activated CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Tait ED,Jordan KA,Dupont CD,Harris TH,Gregg B,Wilson EH,Pepper M,Dzierszinski F,Roos DS,Hunter CA

11-0033 was used in Flow cytometry/Cell sorting to show the dendritic cell response to T.gondii alters levels of CD8+ T cells which ultimately determine the outcome of infection.

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD3e Monoclonal Antibody (eBio500A2 (500A2)), FITC, eBioscience™

CD3e Antibody (11-0033-82) in Flow

Product Details

11-0033-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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