Invitrogen

CD3e Monoclonal Antibody (eBio500A2 (500A2)), eBioscience™

3 Published Figures

25 References

View all (504) CD3 antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

CD3e Antibody (14-0033-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Golden Syrian Hamster IgG Isotype Control Purified (Product # 14-4914-81) (open histogram) or 0.25 µg of Anti-Mouse CD3e Purified (filled histogram) followed by Anti-Syrian Hamster IgG FITC (Product # 11-4211-85). Cells in the lymphocyte gate were used for analysis.

CD3e Antibody in Immunohistochemistry (IHC)

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Product Details

14-0033-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

20 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 14 publications 14 publications

Functional Assay (Functional)

Assay-Dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBio500A2 (500A2)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837128

Product Specific Information

Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Applications Reported: The eBio500A2 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen tissue. It has also been reported for use in in vitro activation of mouse T cells. (Please use Functional Grade purified eBio500A2 in functional assays).

Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBio500A2 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 20 µmL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis.

Nature immunology

Yazicioglu YF,Marin E,Sandhu C,Galiani S,Raza IGA,Ali M,Kronsteiner B,Compeer EB,Attar M,Dunachie SJ,Dustin ML,Clarke AJ

14-0033-82 was used in Immunohistochemistry to show that pharmacological inhibition of mitochondrial transcription and translation inhibits growth of GC-derived human lymphoma cells and induces similar defects in the actin cytoskeleton.

Thu Jun 01 00:00:00 EDT 2023

Essential requirement for polypyrimidine tract binding proteins 1 and 3 in the maturation and maintenance of mature B cells in mice.

European journal of immunology

Monzón-Casanova E,Bates KJ,Smith CWJ,Turner M

14-0033-82 was used in Immunohistochemistry to show that the polypyrimidine tract binding proteins (PTBP) PTBP1 and PTBP3 bind to a large and overlapping set of transcripts in B cells.

Wed Sep 01 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using CD3e monoclonal antibody (Product # 14-0033-82) in Immunohistochemistry

Tue Nov 23 00:00:00 EST 2021

A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice.

Med (New York, N.Y.)

Silva-Cayetano A,Foster WS,Innocentin S,Belij-Rammerstorfer S,Spencer AJ,Burton OT,Fra-Bidó S,Le Lee J,Thakur N,Conceicao C,Wright D,Barrett J,Evans-Bailey N,Noble C,Bailey D,Liston A,Gilbert SC,Lambe T,Linterman MA

14-0033-82 was used in Flow Cytometry to show that ChAdOx1 nCoV-19 induces both cellular and humoral immunity in adult and aged mice and suggest a prime-boost strategy is a rational approach to enhance immunogenicity in older persons.

Fri Mar 12 00:00:00 EST 2021

Critical Adverse Impact of IL-6 in Acute Pneumovirus Infection.

Journal of immunology (Baltimore, Md. : 1950)

Percopo CM,Ma M,Brenner TA,Krumholz JO,Break TJ,Laky K,Rosenberg HF

14-0033 was used in Flow cytometry/Cell sorting to examine the role of the pleiotropic cytokine IL-6 in acute infection with pneumonia virus of mice.

Fri Feb 01 00:00:00 EST 2019

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD3e monoclonal antibody (Product # 14-0033-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Asymmetric PI3K Activity in Lymphocytes Organized by a PI3K-Mediated Polarity Pathway.

Cell reports

Chen YH,Kratchmarov R,Lin WW,Rothman NJ,Yen B,Adams WC,Nish SA,Rathmell JC,Reiner SL

14-0033 was used in Immunocytochemistry-immunoflourescence to show that receptor-associated class I phosphatidylinositol 3-kinase (PI3K) signaling activity, indexed by phosphatidylinositol (3,4,5)-trisphosphate (PIP3) staining, is spatially restricted to the microtubule-organizing center and subsequently to one pole of the mitotic spindle in activated T and B lymphocytes.

Tue Jan 23 00:00:00 EST 2018

Interference with KCTD9 inhibits NK cell activation and ameliorates fulminant liver failure in mice.

BMC immunology

Zhang X,Zhu L,Zhou Y,Shi A,Wang H,Han M,Wan X,Kilonzo SB,Luo X,Chen T,Ning Q

14-0033 was used in Flow cytometry/Cell sorting to propose that target inhibition of KCTD9 may prohibit NK cells activity and thus ameliorate liver damage in viral fulminant hepatitis.

Mon Jun 25 00:00:00 EDT 2018

Mast cells acquire MHCII from dendritic cells during skin inflammation.

The Journal of experimental medicine

Dudeck J,Medyukhina A,Fröbel J,Svensson CM,Kotrba J,Gerlach M,Gradtke AC,Schröder B,Speier S,Figge MT,Dudeck A

14-0033 was used in Flow cytometry/Cell sorting to identify an innate intercellular communication arming resident mast cells with key dendritic cell(DC) functions that might contribute to the acute defence potential during critical periods of migration-based DC absence.

Mon Dec 04 00:00:00 EST 2017

Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence.

Nature

Ibiza S,García-Cassani B,Ribeiro H,Carvalho T,Almeida L,Marques R,Misic AM,Bartow-McKenney C,Larson DM,Pavan WJ,Eberl G,Grice EA,Veiga-Fernandes H

14003382 was used in flow cytometry to explain how glial cells impact ILC3-derived IL-22

Thu Jul 21 00:00:00 EDT 2016

Microbiota regulate the ability of lung dendritic cells to induce IgA class-switch recombination and generate protective gastrointestinal immune responses.

The Journal of experimental medicine

Ruane D,Chorny A,Lee H,Faith J,Pandey G,Shan M,Simchoni N,Rahman A,Garg A,Weinstein EG,Oropallo M,Gaylord M,Ungaro R,Cunningham-Rundles C,Alexandropoulos K,Mucida D,Merad M,Cerutti A,Mehandru S

14-0033 was used in Flow cytometry/Cell sorting to demonstrate the role of the microbiota in modulating the protective efficacy of intranasal vaccination through their effect on the IgA class-switching function of lung dendritic cells.

Mon Jan 11 00:00:00 EST 2016

Bob1 limits cellular frequency of T-follicular helper cells.

European journal of immunology

Yamashita K,Kawata K,Matsumiya H,Kamekura R,Jitsukawa S,Nagaya T,Ogasawara N,Takano K,Kubo T,Kimura S,Shigehara K,Himi T,Ichimiya S

14003382 was used in immunohistochemistry - frozen section to discover that T follicular helper cells preferentially express the transcriptional coactivator Bob1

Wed Jun 01 00:00:00 EDT 2016

Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining.

Bio-protocol

Kumar P,Kolls JK

14-0033 was used in Flow cytometry/Cell sorting to describe the tools, reagents and procdedure for a Th17 cell differentiation method.

Mon Dec 05 00:00:00 EST 2016

T-box Transcription Factors Combine with the Cytokines TGF-β and IL-15 to Control Tissue-Resident Memory T Cell Fate.

Immunity

Mackay LK,Wynne-Jones E,Freestone D,Pellicci DG,Mielke LA,Newman DM,Braun A,Masson F,Kallies A,Belz GT,Carbone FR

14-0033 was used in Flow cytometry/Cell sorting to indicate that the T-box TFs control the two cytokines, TGF-β and IL-15, which are pivotal for CD8(+)CD103(+) Trm cell development and survival.

Tue Dec 15 00:00:00 EST 2015

Dopamine inhibits the function of Gr-1+CD115+ myeloid-derived suppressor cells through D1-like receptors and enhances anti-tumor immunity.

Journal of leukocyte biology

Wu J,Zhang R,Tang N,Gong Z,Zhou J,Chen Y,Chen K,Cai W

14-0033 was used in Functional assays to investigate the therapeutic mechanism and efficacy of DA on the inhibition of cancer development.

Thu Jan 01 00:00:00 EST 2015

Distinct severity of HLH in both human and murine mutants with complete loss of cytotoxic effector PRF1, RAB27A, and STX11.

Blood

Sepulveda FE,Debeurme F,Ménasché G,Kurowska M,Côte M,Pachlopnik Schmid J,Fischer A,de Saint Basile G

14-0033 was used in Functional assays to investigate the effect of mutations in PRF1, RAB27A, and STX11 on hemophagocytic lymphohistiocytosis.

Thu Jan 24 00:00:00 EST 2013

Avirulent Toxoplasma gondii generates therapeutic antitumor immunity by reversing immunosuppression in the ovarian cancer microenvironment.

Cancer research

Baird JR,Fox BA,Sanders KL,Lizotte PH,Cubillos-Ruiz JR,Scarlett UK,Rutkowski MR,Conejo-Garcia JR,Fiering S,Bzik DJ

14-0033 was used in Flow cytometry/Cell sorting to establish that cps preferentially invades tumour-associated antigen-presenting cells and restores their ability to trigger potent anti-tumour CD8(+) T-cell responses.

Mon Jul 01 00:00:00 EDT 2013

TNFR2 is critical for the stabilization of the CD4+Foxp3+ regulatory T. cell phenotype in the inflammatory environment.

Journal of immunology (Baltimore, Md. : 1950)

Chen X,Wu X,Zhou Q,Howard OM,Netea MG,Oppenheim JJ

14-0033 was used in Functional assays to investigate means of promoting stability of Tregs, showing that TNFR2 is critical for stabilisation in the inflammatory environment.

Fri Feb 01 00:00:00 EST 2013

Molecular definition of the identity and activation of natural killer cells.

Nature immunology

Bezman NA,Kim CC,Sun JC,Min-Oo G,Hendricks DW,Kamimura Y,Best JA,Goldrath AW,Lanier LL

14-0033 was used in Flow cytometry/Cell sorting to systematically define the transcriptome of mouse natural killer cells in several contexts.

Mon Oct 01 00:00:00 EDT 2012

IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia.

Nature immunology

Wang Y,Szretter KJ,Vermi W,Gilfillan S,Rossini C,Cella M,Barrow AD,Diamond MS,Colonna M

14-0033 was used in Functional assays to investigate whether IL-34 is responsible for the lack of Langerhans cells and microglia in CSF1R-deficient mice.

Sun Jun 24 00:00:00 EDT 2012

A role for leukocyte-derived IL-1RA in DC homeostasis revealed by increased susceptibility of IL-1RA-deficient mice to cutaneous leishmaniasis.

The Journal of investigative dermatology

Kautz-Neu K,Kostka SL,Dinges S,Iwakura Y,Udey MC,von Stebut E

14-0033 was used in Flow cytometry/Cell sorting to suggest that in addition to effects on Th education, IL-1α/β signalling also modulates DC homeostasis with increased signalling, leading to down-modulation of IL-12 synthesis and worsened disease outcome after infection with Leishmania major.

Mon Aug 01 00:00:00 EDT 2011

Expression of costimulatory TNFR2 induces resistance of CD4+FoxP3- conventional T cells to suppression by CD4+FoxP3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Chen X,Hamano R,Subleski JJ,Hurwitz AA,Howard OM,Oppenheim JJ

14-0033 was used in Functional assays to investigate the role of TNFR2 signalling in the function of conventional T cells.

Thu Jul 01 00:00:00 EDT 2010

Matrilysin (Matrix Metalloproteinase-7) regulates anti-inflammatory and antifibrotic pulmonary dendritic cells that express CD103 (alpha(E)beta(7)-integrin).

The American journal of pathology

Manicone AM,Huizar I,McGuire JK

14-0033 was used in Immunohistochemistry on frozen tissues to assess matrix metalloproteinase-7 effects on CD103-E-cadherin interactions in lung injury.

Tue Dec 01 00:00:00 EST 2009

Matrilysin (Matrix Metalloproteinase-7) regulates anti-inflammatory and antifibrotic pulmonary dendritic cells that express CD103 (alpha(E)beta(7)-integrin).

The American journal of pathology

Manicone AM,Huizar I,McGuire JK

14-0033 was used in Immunohistochemistry on frozen tissues to assess matrix metalloproteinase-7 effects on CD103-E-cadherin interactions in lung injury.

Tue Dec 01 00:00:00 EST 2009

Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease.

The Journal of experimental medicine

Lahl K,Loddenkemper C,Drouin C,Freyer J,Arnason J,Eberl G,Hamann A,Wagner H,Huehn J,Sparwasser T

14-0033 was used in Immunohistochemistry to investigate whether dysfunction or a lack of T reg cells is etiologically involved in scurfy pathogenesis, showing that selective depletion of Foxp3+ Tregs leads to scurfy.

Mon Jan 22 00:00:00 EST 2007

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD3e Monoclonal Antibody (eBio500A2 (500A2)), eBioscience™

CD3e Antibody (14-0033-82) in Flow

Product Details

14-0033-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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